Nkd2 promotes the differentiation of dental follicle stem/progenitor cells into osteoblasts

Chen,Chanchan; Zhang,Jianying; Ling,Junqi; Du,Yu; Hou,Yuluan

doi:10.3892/ijmm.2018.3822

Nkd2 promotes the differentiation of dental follicle stem/progenitor cells into osteoblasts

Authors:
- Chanchan Chen
- Jianying Zhang
- Junqi Ling
- Yu Du
- Yuluan Hou
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Affiliations: Department of Stomatology, Shenzhen Children's Hospital, Shenzhen, Guangdong 518038, P.R. China, Department of Operative Dentistry and Endodontics, Xiangya School of Stomatology, Xiangya Stomatological Hospital, Central South University, Changsha, Hu'nan 410083, P.R. China, Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Research Institute of Stomatology, Guangdong Province Key Laboratory of Stomatology, Sun Yat‑sen University, Guangzhou, Guangdong 510055, P.R. China
Published online on: August 14, 2018 https://doi.org/10.3892/ijmm.2018.3822
Pages: 2403-2414
Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Dental follicle stem/progenitor cells have the potential to undergo osteogenesis. naked cuticle homolog 2 (Nkd2) is a signal‑inducible feedback antagonist of the canonical Wnt signaling pathway. The purpose of the present study was to investigate the function of Nkd2 in the differentiation of dental follicle stem/progenitor cells (DFSCs) into osteoblasts. Immunohistochemistry, reverse transcription‑quantitative polymerase chain reaction and western blotting were employed to detect Nkd2 expression in rat DFSCs. In addition, rat DFSCs (rDFSCs) were transfected with small interfering RNAs to examine the effect of Nkd2 on the differentiation of these cells into osteoblasts. Furthermore, the function of Nkd2 in the Wnt/β‑catenin pathway in rDFSCs was investigated using β‑catenin/T‑cell factor luciferase activity assays and western blotting. It was revealed that the expression of Nkd2 was upregulated during the differentiation of rDFSCs into osteoblasts. Furthermore, osteoblast differentiation ability and Wnt/β‑catenin pathway activity were significantly decreased in Nkd2‑silenced rDFSCs compared with the si‑NC group (P<0.05 and P<0.001, respectively). The results suggest that Nkd2 promotes the differentiation of rDFSCs into osteoblasts through Wnt/β‑catenin signaling.

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