Distinct expression of C4.4A in colorectal cancer detected by different antibodies Corrigendum in /10.3892/ijo.2016.3694

Yamamoto,Hirofumi; Oshiro,Ryota; Ohtsuka,Masahisa; Uemura,Mamoru; Haraguchi,Naotsugu; Nishimura,Junichi; Takemasa,Ichiro; Mizushima,Tsunekazu; Doki,Yuichiro; Mori,Masaki

doi:10.3892/ijo.2012.1714

Distinct expression of C4.4A in colorectal cancer detected by different antibodies

Corrigendum in: /10.3892/ijo.2016.3694

Authors:
- Hirofumi Yamamoto
- Ryota Oshiro
- Masahisa Ohtsuka
- Mamoru Uemura
- Naotsugu Haraguchi
- Junichi Nishimura
- Ichiro Takemasa
- Tsunekazu Mizushima
- Yuichiro Doki
- Masaki Mori
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Affiliations: Department of Surgery, Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan
Published online on: November 22, 2012 https://doi.org/10.3892/ijo.2012.1714
Pages: 197-201

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Abstract

The metastasis-associated gene C4.4A encodes a glycolipid-anchored membrane protein expressed in several human malignancies. The present study aimed to perform a detailed assessment of C4.4A expression in colorectal cancer tissues, in terms of intra-cellular localization, intra-tumoral location and difference in molecular weight. To advance this goal, we developed three new antibodies against the C4.4A protein (two polyclonal Abs: C4.4A-119 and C4.4A-277 and one monoclonal Ab: C4.4A GPI-M) to use in addition to the two previously produced polyclonal Abs (C4.4A-81, C4.4A GPI-P). Antibody specificities were confirmed by absorption tests. Western blot analysis and immunohistochemistry showed that the C4.4A-119 and C4.4-277 Abs detected 70-kDa C4.4A, mainly in the cytoplasm, irrespective of intra-tumoral location. The C4.4A GPI-P and C4.4A GPI-M Abs reacted with the membranous ~40-kDa C4.4A, exclusively at the tumor invasive front, and each detected an identical tumor cell population. The tested antibodies showed varied C4.4A detection rates in 33 CRC tissues. The C4.4A-277 Ab yielded the highest positive rate in 29 of 33 CRC tissues (87.9%), while the C4.4A GPI-P and C4.4A GPI-M Abs each only showed 33.3% positivity. The present findings suggest that the GPI anchor signaling sequence may be essential for detecting membranous C4.4A at the invasive front of CRC tissues.

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