miRNA-99b-3p functions as a potential tumor suppressor by targeting glycogen synthase kinase-3β in oral squamous cell carcinoma Tca-8113 cells

  • Authors:
    • Kang He
    • Dongdong Tong
    • Si Zhang
    • Donghui Cai
    • Lumin Wang
    • Yang Yang
    • Ling Gao
    • Su'e Chang
    • Bo Guo
    • Tusheng Song
    • Ang Li
    • Chen Huang
  • View Affiliations

  • Published online on: August 27, 2015     https://doi.org/10.3892/ijo.2015.3135
  • Pages: 1528-1536
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Abstract

Dysregulation of microRNAs (miRNAs) has been associated with carcinogenesis in oral squamous cell carcinoma (OSCC). In the present study, we investigated the expression and function of miR-99b-3p in human OSCC. We found that the expression levels of miR-99b-3p decreased in 21 clinical OSCC samples (84%). Furthermore, ectopic expression of miR-99b-3p inhibited OSCC cell proliferation by downregulating glycogen synthase kinase-3β (GSK3β), an miR-99b-3p' target gene, at the mRNA and protein levels, both in vitro and in vivo. Moreover, the silencing of GSK3β recapitulated the cellular and molecular effects in a similar manner to the overexpression of miR-99b-3p, which included inhibition of OSCC cell proliferation and suppression of p65 (RelA) and G1 regulators (cyclin D1, CDK4 and CDK6) in vitro. Our data suggest that miR-99b-3p functions as a tumor suppressor in OSCC via GSK3β downregulation.
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October-2015
Volume 47 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
He K, Tong D, Zhang S, Cai D, Wang L, Yang Y, Gao L, Chang S, Guo B, Song T, Song T, et al: miRNA-99b-3p functions as a potential tumor suppressor by targeting glycogen synthase kinase-3β in oral squamous cell carcinoma Tca-8113 cells. Int J Oncol 47: 1528-1536, 2015
APA
He, K., Tong, D., Zhang, S., Cai, D., Wang, L., Yang, Y. ... Huang, C. (2015). miRNA-99b-3p functions as a potential tumor suppressor by targeting glycogen synthase kinase-3β in oral squamous cell carcinoma Tca-8113 cells. International Journal of Oncology, 47, 1528-1536. https://doi.org/10.3892/ijo.2015.3135
MLA
He, K., Tong, D., Zhang, S., Cai, D., Wang, L., Yang, Y., Gao, L., Chang, S., Guo, B., Song, T., Li, A., Huang, C."miRNA-99b-3p functions as a potential tumor suppressor by targeting glycogen synthase kinase-3β in oral squamous cell carcinoma Tca-8113 cells". International Journal of Oncology 47.4 (2015): 1528-1536.
Chicago
He, K., Tong, D., Zhang, S., Cai, D., Wang, L., Yang, Y., Gao, L., Chang, S., Guo, B., Song, T., Li, A., Huang, C."miRNA-99b-3p functions as a potential tumor suppressor by targeting glycogen synthase kinase-3β in oral squamous cell carcinoma Tca-8113 cells". International Journal of Oncology 47, no. 4 (2015): 1528-1536. https://doi.org/10.3892/ijo.2015.3135