Lippia origanoides extract induces cell cycle arrest and apoptosis and suppresses NF-κB signaling in triple-negative breast cancer cells

  • Authors:
    • Vishak Raman
    • Jorge Luis Fuentes Lorenzo
    • Elena E. Stashenko
    • Morris Levy
    • Maria M. Levy
    • Ignacio G. Camarillo
  • View Affiliations

  • Published online on: October 19, 2017     https://doi.org/10.3892/ijo.2017.4169
  • Pages:1801-1808
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Abstract

Treatments targeting hormone receptors typically fail to provide a positive clinical outcome against triple-negative breast cancers (TNBC), which lack expression of estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor 2 (Her2/neu). Towards identifying viable treatments for aggressive breast cancer, we have tested an extract of the tropical plant Lippia origanoides (LOE) on TNBC and normal cells lines to uncover its potential anticancer effects. Treatment with LOE reduced TNBC cell viability in a dose-dependent manner to a greater extent than in normal mammary epithelial MCF10A cells. In MDA-MB‑231 cells, LOE was found to halt the cell cycle in the G0/G1 phase via cyclin D1 and cIAP2 regulation, and induce apoptosis without promoting necrosis via caspase-8/-3 and PARP cleavage. Constitutive nuclear factor-κB (NF-κB) signaling has been shown to contribute to the heightened inflammatory state and survival in TNBC cells. Herein, we also provide evidence that LOE inhibits NF-κB signaling by reducing RIP1 protein levels in MDA-MB-231 cells. These studies reveal that LOE suppresses key features of the progression of aggressive breast cancer cells and provides a basis for further definition of its underlying mechanisms of action and anticancer potential.

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December 2017
Volume 51 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

2016 Impact Factor: 3.079
Ranked #33/217 Oncology
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APA
Raman, V., Fuentes Lorenzo, J.L., Stashenko, E.E., Levy, M., Levy, M.M., & Camarillo, I.G. (2017). Lippia origanoides extract induces cell cycle arrest and apoptosis and suppresses NF-κB signaling in triple-negative breast cancer cells. International Journal of Oncology, 51, 1801-1808. https://doi.org/10.3892/ijo.2017.4169
MLA
Raman, V., Fuentes Lorenzo, J. L., Stashenko, E. E., Levy, M., Levy, M. M., Camarillo, I. G."Lippia origanoides extract induces cell cycle arrest and apoptosis and suppresses NF-κB signaling in triple-negative breast cancer cells". International Journal of Oncology 51.6 (2017): 1801-1808.
Chicago
Raman, V., Fuentes Lorenzo, J. L., Stashenko, E. E., Levy, M., Levy, M. M., Camarillo, I. G."Lippia origanoides extract induces cell cycle arrest and apoptosis and suppresses NF-κB signaling in triple-negative breast cancer cells". International Journal of Oncology 51, no. 6 (2017): 1801-1808. https://doi.org/10.3892/ijo.2017.4169