Long non-coding RNA CASC2 inhibits breast cancer cell growth and metastasis through the regulation of the miR-96-5p/SYVN1 pathway

Gao,Zejun; Wang,Hai; Li,Hangyu; Li,Min; Wang,Jia; Zhang,Wenwen; Liang,Xiubin; Su,Dongming; Tang,Jinhai

doi:10.3892/ijo.2018.4522

Long non-coding RNA CASC2 inhibits breast cancer cell growth and metastasis through the regulation of the miR-96-5p/SYVN1 pathway

Authors:
- Zejun Gao
- Hai Wang
- Hangyu Li
- Min Li
- Jia Wang
- Wenwen Zhang
- Xiubin Liang
- Dongming Su
- Jinhai Tang
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Affiliations: Department of General Surgery, The First Afﬁliated Hospital with Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China, Department of Pathology, Nanjing Medical University, Nanjing, Jiangsu 211166, P.R. China, Department of General Surgery, The Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning 110032, P.R. China, Department of General Surgery, The Second Afﬁliated Hospital with Dalian Medical University, Dalian, Liaoning 116027, P.R. China, Department of Clinical Laboratory, Children's Hospital of Nanjing Medical University, Nanjing, Jiangsu 210008, P.R. China
Published online on: August 13, 2018 https://doi.org/10.3892/ijo.2018.4522
Pages: 2081-2090

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Abstract

Cancer susceptibility candidate 2 (CASC2), a long non-coding RNA (lncRNA), has been demonstrated to be a tumor suppressor in several types of cancer. However, the role and mechanism of CASC2 in breast cancer (BC) have not been investigated. In the present study, the expression and functions of CASC2 in BC were investigated. The expression of CASC2 was significantly decreased in BC tissues and cells compared with adjacent normal tissues and mammary epithelial cells, respectively. CASC2 overexpression inhibited the viability, migration and invasion, and elevated apoptosis of BC cells. In addition, CASC2 acted as a competing endogenous RNA for hsa-microRNA (miR)-96-5p and regulated the expression of its target gene, synoviolin (SYVN1). In miR-96-5p-overexpressed MDA-MB-231 cells, cell viability, migration and invasion was increased, and cell apoptosis was decreased, which was reversed by the upregulation of SYVN1. Taken together, the present study data indicated that decreased SYVN1 expression was a tumor suppressor, which inhibited the growth and metastasis of BC through the miR-96-5p/SYVN1 axis.

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