Spectrum construction of differentially expressed circular RNAs in patients with leukoaraiosis and function analysis of differentially expressed genes

Mi,Te; Luo,Changjiang; Hu,Yawei; Qu,Chuanqiang; Wang,Xiang; Guo,Shougang; Du,Yifeng

doi:10.3892/mmr.2017.6871

Spectrum construction of differentially expressed circular RNAs in patients with leukoaraiosis and function analysis of differentially expressed genes

Authors:
- Te Mi
- Changjiang Luo
- Yawei Hu
- Chuanqiang Qu
- Xiang Wang
- Shougang Guo
- Yifeng Du
View Affiliations

Affiliations: Department of ICU, Jining No. 1 People's Hospital, Jining, Shandong 272000, P.R. China, Department of Neurology, Provincial Hospital Affiliated to Shandong University (South Branch), Jinan, Shandong 250002, P.R. China, Department of Neurosurgery, Affiliated Hospital of Jining Medical University Jining, Shandong 272029, P.R. China, Department of Neurology, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China
Published online on: June 28, 2017 https://doi.org/10.3892/mmr.2017.6871
Pages: 2563-2569
Copyright: © Mi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Circular RNAs (circRNAs) are class of endogenous RNAs that have a role in the regulation of gene expression. The present study aimed to investigate the diagnostic value and role of circRNA in the pathogenesis of leukoaraiosis (LA). The present study performed Arraystar Human circRNA Array analysis of 6 samples from LA cases and 6 samples from control cases. Differentially expressed (DE) circRNAs between two samples were identified through fold‑change (>1.5‑fold) screening. Afterwards, based on DE circRNAs, the gene ontology (GO) analysis of upregulated DE genes identified from DE circRNAs demonstrated that DE genes were primarily associated with cellular metabolic processes, membrane‑bound organelles and binding. However, none were enriched in the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. Downregulated DE genes were enriched in cellular localization, cytoplasm and kinase binding. For the KEGG pathways, the downregulated DE genes were primarily associated with the insulin signaling pathway. The results of the present study indicated that the DE genes from differently expressed circRNAs may have an important role in the pathogenesis of LA and may be a novel targfet for further research.

View Figures

View References

1	Lin Q, Huang WQ and Tzeng CM: Genetic associations of leukoaraiosis indicate pathophysiological mechanisms in white matter lesions etiology. Rev Neurosci. 26:343–358. 2015. View Article : Google Scholar : PubMed/NCBI
2	Ben-Assayag E, Mijajlovic M, Shenhar-Tsarfaty S, Bova I, Shopin L and Bornstein NM: Leukoaraiosis is a chronic atherosclerotic disease. ScientificWorldJournal. 2012:5321412012. View Article : Google Scholar : PubMed/NCBI
3	Memczak S, Jens M, Elefsinioti A, Torti F, Krueger J, Rybak A, Maier L, Mackowiak SD, Gregersen LH, Munschauer M, et al: Circular RNAs are a large class of animal RNAs with regulatory potency. Nature. 495:333–338. 2013. View Article : Google Scholar : PubMed/NCBI
4	Chen YT, Rettig WJ, Yenamandra AK, Kozak CA, Chaganti RS, Posner JB and Old LJ: Cerebellar degeneration-related antigen: A highly conserved neuroectodermal marker mapped to chromosomes X in human and mouse. Proc Natl Acad Sci USA. 87:3077–3081. 1990. View Article : Google Scholar : PubMed/NCBI
5	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2 (−Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
6	Salzman J, Gawad C, Wang PL, Lacayo N and Brown PO: Circular RNAs are the predominant transcript isoform from hundreds of human genes in diverse cell types. PLoS One. 7:e307332012. View Article : Google Scholar : PubMed/NCBI
7	Simoni M, Li L, Paul NL, Gruter BE, Schulz UG, Küker W and Rothwell PM: Age- and sex-specific rates of leukoaraiosis in TIA and stroke patients: Population-based study. Neurology. 79:1215–1222. 2012. View Article : Google Scholar : PubMed/NCBI
8	Uh J, Yezhuvath U, Cheng Y and Lu H: In vivo vascular hallmarks of diffuse leukoaraiosis. J Magn Reson Imaging. 32:184–190. 2010. View Article : Google Scholar : PubMed/NCBI
9	Avet J, Pichot V, Barthélémy JC, Laurent B, Garcin A, Roche F and Celle S: Leukoaraiosis and ambulatory blood pressure load in a healthy elderly cohort study: The PROOF study. Int J Cardiol. 172:59–63. 2014. View Article : Google Scholar : PubMed/NCBI
10	Kim SH, Shim JY, Lee HR, Na HY and Lee YJ: The relationship between pulse pressure and leukoaraiosis in the elderly. Arch Gerontol Geriatr. 54:206–209. 2012. View Article : Google Scholar : PubMed/NCBI
11	Guo JU, Agarwal V, Guo H and Bartel DP: Expanded identification and characterization of mammalian circular RNAs. Genome Biol. 15:4092014. View Article : Google Scholar : PubMed/NCBI
12	Jeck WR, Sorrentino JA, Wang K, Slevin MK, Burd CE, Liu J, Marzluff WF and Sharpless NE: Circular RNAs are abundant, conserved, and associated with ALU repeats. RNA. 19:141–157. 2013. View Article : Google Scholar : PubMed/NCBI
13	Hansen TB, Jensen TI, Clausen BH, Bramsen JB, Finsen B, Damgaard CK and Kjems J: Natural RNA circles function as efficient microRNA sponges. Nature. 495:384–388. 2013. View Article : Google Scholar : PubMed/NCBI
14	Danan M, Schwartz S, Edelheit S and Sorek R: Transcriptome-wide discovery of circular RNAs in Archaea. Nucleic Acids Res. 40:3131–3142. 2012. View Article : Google Scholar : PubMed/NCBI
15	Valdmanis PN and Kay MA: The expanding repertoire of circular RNAs. Mol Ther. 21:1112–1114. 2013. View Article : Google Scholar : PubMed/NCBI
16	Hentze MW and Preiss T: Circular RNAs: Splicing's enigma variations. EMBO J. 32:923–925. 2013. View Article : Google Scholar : PubMed/NCBI
17	Junn E, Lee KW, Jeong BS, Chan TW, Im JY and Mouradian MM: Repression of alpha-synuclein expression and toxicity by microRNA-7. Proc Natl Acad Sci USA. 106:13052–13057. 2009. View Article : Google Scholar : PubMed/NCBI
18	Lukiw WJ: Circular RNA (circRNA) in Alzheimer's disease (AD). Front Genet. 4:3072013. View Article : Google Scholar : PubMed/NCBI
19	Namgaladze D and Brüne B: Phospholipase A2-modified low-density lipoprotein activates the phosphatidylinositol 3-kinase-Akt pathway and increases cell survival in monocytic cells. Arterioscler Thromb Vasc Biol. 26:2510–2516. 2006. View Article : Google Scholar : PubMed/NCBI
20	Northcott CA, Hayflick JS and Watts SW: PI3-kinase upregulation and involvement in spontaneous tone in arteries from DOCA-salt rats: Is p110delta the culprit? Hypertension. 43:885–890. 2004. View Article : Google Scholar : PubMed/NCBI