Open Access

Overexpression of NAC1 confers drug resistance via HOXA9 in colorectal carcinoma cells

  • Authors:
    • Tongfa Ju
    • Huicheng Jin
    • Rongchao Ying
    • Qi Xie
    • Chunhua Zhou
    • Daquan Gao
  • View Affiliations

  • Published online on: July 14, 2017     https://doi.org/10.3892/mmr.2017.6986
  • Pages: 3194-3200
  • Copyright: © Ju et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Colorectal carcinoma (CRC) is one of the most common types of malignancy worldwide. Recently, neoadjuvant chemotherapy has become an important treatment strategy for CRC. However, treatment frequently fails due to the development of chemoresistance, which is a major obstacle for positive prognosis. However, the underlying mechanisms of chemoresistance remain unclear. The present study assessed the functions of nucleus accumbens‑associated protein 1 (NAC1), an important transcriptional regulator, in CRC progression. Reverse transcription‑quantitative polymerase chain reaction, western blot analysis and immunohistochemistry were performed to detect the expression levels of NAC1. It was identified that NAC1 was significantly overexpressed in CRC compared with non‑tumorous tissues, indicating an oncogenic role. Following this, gain and loss of function analyses were performed in vitro to further investigate the function of NAC1. Cell viability and caspase‑3/7 activity assays were used to assess chemotherapy‑induced apoptosis. These results indicated that overexpression of NAC1 in CRC cells increased resistance to chemotherapy and inhibited apoptosis. Additionally, RNA interference‑mediated knockdown of NAC1 restored the chemosensitivity of CRC cells. Furthermore, mechanistic investigation revealed that NAC1 increased drug resistance via inducing homeobox A9 (HOXA9) expression, and that knockdown of HOXA9 abrogated NAC1‑induced drug resistance. In conclusion, the results of the present study demonstrated that NAC1 may be a critical factor in the develo­pment of chemoresistance, offering a potential novel target for the treatment of CRC.
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September-2017
Volume 16 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Ju T, Jin H, Ying R, Xie Q, Zhou C and Gao D: Overexpression of NAC1 confers drug resistance via HOXA9 in colorectal carcinoma cells. Mol Med Rep 16: 3194-3200, 2017
APA
Ju, T., Jin, H., Ying, R., Xie, Q., Zhou, C., & Gao, D. (2017). Overexpression of NAC1 confers drug resistance via HOXA9 in colorectal carcinoma cells. Molecular Medicine Reports, 16, 3194-3200. https://doi.org/10.3892/mmr.2017.6986
MLA
Ju, T., Jin, H., Ying, R., Xie, Q., Zhou, C., Gao, D."Overexpression of NAC1 confers drug resistance via HOXA9 in colorectal carcinoma cells". Molecular Medicine Reports 16.3 (2017): 3194-3200.
Chicago
Ju, T., Jin, H., Ying, R., Xie, Q., Zhou, C., Gao, D."Overexpression of NAC1 confers drug resistance via HOXA9 in colorectal carcinoma cells". Molecular Medicine Reports 16, no. 3 (2017): 3194-3200. https://doi.org/10.3892/mmr.2017.6986