Protective and therapeutic effects of Danhong injection on acute pancreatitis‑associated lung injury

Liu,Qin; Hua,Fuzhou; Deng,Changqing; Zhang,Jing; Xu,Guohai; Hu,Yanhui

doi:10.3892/mmr.2017.7526

Protective and therapeutic effects of Danhong injection on acute pancreatitis‑associated lung injury

Authors:
- Qin Liu
- Fuzhou Hua
- Changqing Deng
- Jing Zhang
- Guohai Xu
- Yanhui Hu
View Affiliations

Affiliations: Department of Anesthesiology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China, Department of Gastroenterology, The Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi 330000, P.R. China
Published online on: September 19, 2017 https://doi.org/10.3892/mmr.2017.7526
Pages: 7603-7608

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Lung functional impairment caused by acute pancreatitis (AP) is the primary contributor to AP‑associated mortality. Previous studies have reported that AP‑associated lung injury is associated with systemic inflammatory response syndrome and oxidative stress. In the present study, the protective effects of Danhong injection (DHI), a widely used Chinese Traditional Medicine preparation, on AP‑associated lung injury in rats was examined. The myeloperoxidase activity, malondiadelhyde level and superoxide dismutase activity determination demonstrated the anti‑inflammatory and anti‑oxidative properties of DHI. The results of western blotting and reverse‑transcription‑semi‑quantitative polymerase chain reaction indicated that DHI could protect rats against AP‑associated lung injury, and the protective effect was associated with the suppression of nuclear factor‑κB activation and cell adhesion molecule expression, and the reduction of neutrophil infiltration and oxidative stress levels. As demonstrated by HE staining, DHI inhibited the pancreas and lung tissue injury. Therefore, DHI could be a potential candidate for the treatment of patients with AP‑associated lung injury.

View Figures

View References

1	Lund H, Tønnesen H, Tønnesen MH and Olsen O: Long-term recurrence and death rates after acute pancreatitis. Scand J Gastroentero. 41:234–238. 2006. View Article : Google Scholar
2	Akbarshahi H, Rosendahl AH, Westergren-Thorsson G and Andersson R: Acute lung injury in acute pancreatitis-awaiting the big leap. Resp Med. 106:1199–1210. 2012. View Article : Google Scholar
3	Bhatia M, Brady M, Shokuhi S, Christmas S, Neoptolemos JP and Slavin J: Inflammatory mediators in acute pancreatitis. J Pathol. 190:117–125. 2000. View Article : Google Scholar : PubMed/NCBI
4	Norman J: The role of cytokines in the pathogenesis of acute pancreatitis. Am J Surg. 175:76–83. 1998. View Article : Google Scholar : PubMed/NCBI
5	Lane JS, Todd KE, Gloor B, Chandler CF, Kau AW, Ashley SW and McFadden DW: Platelet activating factor antagonism reduces the systemic inflammatory response in a murine model of acute pancreatitis. J Surg Res. 99:365–370. 2001. View Article : Google Scholar : PubMed/NCBI
6	Shabanov VV, Sarbaeva NN and Milyakova MN: Generation of free oxygen radicals in the pathogenesis of experimental acute reflux pancreatitis. Bull Exp Biol Med. 134:26–27. 2002. View Article : Google Scholar : PubMed/NCBI
7	Eşrefoğlu M, Gül M, Ates B, Batçioğlu K and Selimoğlu MA: Antioxidative effect of melatonin, ascorbic acid and N-acetylcysteine on caerulein-induced pancreatitis and associated liver injury in rats. World J Gastroentero. 12:259–264. 2006. View Article : Google Scholar
8	Lapidot T, Walker MD and Kanner J: Antioxidant and prooxidant effects of phenolics on pancreatic β-cells in vitro. J Agr Food Chem. 50:7220–7225. 2002. View Article : Google Scholar
9	Sun M, Zhang JJ, Shan JZ, Zhang H, Jin CY, Xu S and Wang YL: Clinical observation of Danhong Injection (herbal TCM product from Radix Salviae miltiorrhizae and Flos Carthami tinctorii) in the treatment of traumatic intracranial hematoma. Phytomedicine. 16:683–689. 2009. View Article : Google Scholar : PubMed/NCBI
10	He Y, Wan H, Du Y, Bie X, Zhao T, Fu W and Xing P: Protective effect of Danhong injection on cerebral ischemia-reperfusion injury in rats. J Ethnopharmacol. 144:387–394. 2012. View Article : Google Scholar : PubMed/NCBI
11	Gao LN, Cui YL, Wang QS and Wang SX: Amelioration of Danhong injection on the lipopolysaccharide-stimulated systemic acute inflammatory reaction via multi-target strategy. J Ethnopharmacol. 149:772–782. 2013. View Article : Google Scholar : PubMed/NCBI
12	Huang YM, Zhang ZJ, Guo AM, Pan LH and Li CE: Determination of protocatechuic aldehyde in Danhong injection by HPLC. Jie Fang Jun Yao Xue Xue Bao Bian Ji Bu. 21:467–469. 2005.(In Chinese).
13	Wang SM: Determination of water-solubility component in Danhong injection by HPLC. Shi Zhen Guo Yi Guo Yao Bian Ji Bu. 17:989–990. 2006.(In Chinese).
14	Liu X, Wu Z, Yang K, Ding H and Wu Y: Quantitative analysis combined with chromatographic fingerprint for comprehensive evaluation of Danhong injection using HPLC-DAD. J Pharm Biomed Anal. 76:70–74. 2013. View Article : Google Scholar : PubMed/NCBI
15	Wan LH, Chen J, Li L, Xiong WB and Zhou LM: Protective effects of Carthamus tinctorius injection on isoprenaline-induced myocardial injury in rats. Pharm Biol. 49:1204–1209. 2011. View Article : Google Scholar : PubMed/NCBI
16	Chen YH, Lin SJ, Ku HH, Shiao MS, Lin FY, Chen JW and Chen YL: Salvianolic acid B attenuates VCAM-1 and ICAM-1 expression in TNF-alpha-treated human aortic endothelial cells. J Cell Biochem. 82:512–521. 2001. View Article : Google Scholar : PubMed/NCBI
17	Chen ZQ, Hong L and Wang H: Effect of Danhong injection on platelet activation and inflammatory factors in patients of acute coronary syndrome after intervention therapy. Zhongguo Zhong Xi Yi Jie He Za Zhi. 29:692–694. 2009.(In Chinese). PubMed/NCBI
18	Wang L, Zhang X, Liu L, Cui L, Yang R, Li M and Du W: Tanshinone II A down-regulates HMGB1, RAGE, TLR4, NF-κappaB expression, ameliorates BBB permeability and endothelial cell function and protects rat brains against focal ischemia. Brain Res. 1321:143–151. 2010. View Article : Google Scholar : PubMed/NCBI
19	Perides G, van Acker GJ, Laukkarinen JM and Steer ML: Experimental acute biliary pancreatitis induced by retrograde infusion of bile acids into the mouse pancreatic duct. Nat Protoc. 5:335–341. 2010. View Article : Google Scholar : PubMed/NCBI
20	Schmidt J, Rattner DW, Lewandrowski K, Compton CC, Mandavilli U, Knoefel WT and Warshaw AL: A better model of acute pancreatitis for evaluating therapy. Ann Surg. 215:44–56. 1992. View Article : Google Scholar : PubMed/NCBI
21	Tanino Y, Makita H, Miyamoto K, Betsuyaku T, Ohtsuka Y, Nishihira J and Nishimura M: Role of macrophage migration inhibitory factor in bleomycin-induced lung injury and fibrosis in mice. Am J Physiol Lung Cell Mol Physiol. 283:L156–L162. 2002. View Article : Google Scholar : PubMed/NCBI
22	Simms D, Cizdziel PE and Chomczynski P: TRIzol: A new reagent for optimal single-step isolation of RNA. Focus. 15:532–535. 1993.
23	Yoshida M and Yamada M, Sudo Y, Kojima T, Tomiyasu T, Yoshikawa N, Oda T and Yamada M: Myeloperoxidase anti-neutrophil cytoplasmic antibody affinity is associated with the formation of neutrophil extracellular traps in the kidney and vasculitis activity in myeloperoxidase anti-neutrophil cytoplasmic antibody-associated microscopic polyangiitis. Nephrology (Carlton). 21:624–629. 2016. View Article : Google Scholar : PubMed/NCBI
24	Medzhitov R and Horng T: Transcriptional control of the inflammatory response. Nat Rev Immunol. 9:692–703. 2009. View Article : Google Scholar : PubMed/NCBI
25	Pooran N, Indaram A, Singh P and Bank S: Cytokines (IL-6, IL-8, TNF): Early and reliable predictors of severe acute pancreatitis. J Clin Gastroenterol. 37:263–266. 2003. View Article : Google Scholar : PubMed/NCBI
26	Boone DL, Lee EG, Libby S, Gibson PJ, Chien M, Chan F, Madonia M, Burkett PR and Ma A: Recent advances in understanding NF-κappaB regulation. Inflamm Bowel Dis. 8:201–212. 2002. View Article : Google Scholar : PubMed/NCBI
27	Shi C, Zhao X, Lagergren A, Sigvardsson M, Wang X and Andersson R: Immune status and inflammatory response differ locally and systemically in severe acute pancreatitis. Scand J Gastroenterol. 41:472–480. 2006. View Article : Google Scholar : PubMed/NCBI
28	Meng Y, Ma QY, Kou XP and Xu J: Effect of resveratrol on activation of nuclear factor kappa-B and inflammatory factors in rat model of acute pancreatitis. World J Gastroenterol. 11:525–528. 2005. View Article : Google Scholar : PubMed/NCBI
29	Leung PS and Chan YC: Role of oxidative stress in pancreatic inflammation. Antioxid Redox Signal. 11:135–166. 2009. View Article : Google Scholar : PubMed/NCBI
30	Yu JH, Lim JW, Kim KH, Morio T and Kim H: NADPH oxidase and apoptosis in cerulein-stimulated pancreatic acinar AR42J cells. Free Radical Bio Med. 39:590–602. 2005. View Article : Google Scholar
31	Seo JY, Kim H, Seo JT and Kim KH: Oxidative stress induced cytokine production in isolated rat pancreatic acinar cells: Effects of small-molecule antioxidants. Pharmacology. 64:63–70. 2002. View Article : Google Scholar : PubMed/NCBI