(R)‑dehydroxyabscisic alcohol β‑D‑apiofuranosyl‑(1ˮ→6ʼ)‑β‑D‑glucopyranoside enhances the osteoblastic differentiation of ST2 cells via the BMP/WNT pathways

Liu,Yadong; Yang,Tao; Chen,Ting; Hao,Jun; Gai,Yu; Zhang,Weiguo

doi:10.3892/mmr.2018.9690

(R)‑dehydroxyabscisic alcohol β‑D‑apiofuranosyl‑(1ˮ→6ʼ)‑β‑D‑glucopyranoside enhances the osteoblastic differentiation of ST2 cells via the BMP/WNT pathways

Authors:
- Yadong Liu
- Tao Yang
- Ting Chen
- Jun Hao
- Yu Gai
- Weiguo Zhang
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Affiliations: Department of Joint Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China, Department of Joint Surgery and Sports Medicine, Tianjin Union Medical Center, Tianjin 300121, P.R. China, Department of Orthopedics, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China, Department of Pathology, Dalian Municipal Central Hospital Affiliated to Dalian Medical University, Dalian, Liaoning 116033, P.R. China
Published online on: November 23, 2018 https://doi.org/10.3892/mmr.2018.9690
Pages: 461-467
Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Lonicera japonica has been used in traditional Chinese medicine as an important medicinal plant, with the ability to inhibit osteoclast development and bone loss. However, it is not clear which active ingredient exerts these effects. (R)‑dehydroxyabscisic alcohol β‑D‑apiofuranosyl‑(1ˮ→6ʼ)‑β‑D‑glucopyranoside (DAG) is an active constituent isolated from Lonicera japonica. In the present study, the ST2 bone marrow stromal cell line was treated by DAG at different concentrations and the osteoblastic differentiation was explored by ELISA assay, Von Kossa staining, Alizarin Red S staining, reverse transcription‑quantitative polymerase chain reaction and western blot analysis. The results revealed that DAG promoted osteoblastic differentiation, as evidenced by increasing mineralization and alkaline phosphatase (ALP) activity, as well as the expression of genes encoding bone differentiation markers, including Alp, osteopontin (Opn) and osteocalcin (Ocn). In addition, DAG upregulated the gene expression of bone morphogenetic protein (Bmp)‑2, Bmp4, Wnt family member (Wnt)‑1, Wnt3 and runt‑related transcription factor 2 (Runx2), as well as the protein expression of phosphorylated‑mothers against decapentaplegic homolog (Smad) 1, Smad5 Smad8, β‑catenin and Runx2 in ST2 cells. The osteogenic effects induced by DAG were attenuated by the BMP antagonist Noggin and the WNT signaling pathway inhibitor Dickkopf related protein‑1. The data indicated that DAG promoted the osteoblastic differentiation of ST2 cells, at least partially through regulating the BMP/WNT signaling pathways. This provides scientific rationale for the development of DAG as a treatment for bone loss‑associated diseases, such as osteoporosis.

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