Effects of clinical isolates of Streptococcus pneumoniae on THP-1 human monocytic cells

  • Authors:
    • Jin Zhang
    • Da-Kang Hu
    • Dong-Guo Wang
    • Yang Liu
    • Chi-Bo Liu
    • Lian-Hua Yu
    • Ying Qu
    • Xin-Hua Luo
    • Jin-Hong Yang
    • Jian Yu
    • Shuang‑Chun Liu
    • Xiang-Yang Li
  • View Affiliations

  • Published online on: September 17, 2013     https://doi.org/10.3892/mmr.2013.1688
  • Pages: 1570-1574
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Abstract

Twenty‑three clinical Streptococcus pneumoniae (SP) strains were isolated from blood and sputum specimens from the Second Affiliated Hospital of Wenzhou Medical College in 2009. These strains and the ATCC 49619 standard strain were cultured and suspended in normal saline (at a turbidity of 1.0 McFarland). The production of interleukin (IL)‑8, intracellular adhesion molecule‑1 (ICAM‑1) and IL‑10 in THP‑1 cells following stimulation with the SP suspension was analyzed by an enzyme-linked immunosorbent assay. The concentrations of IL‑8, ICAM‑1 and IL‑10 from the THP‑1 monocytes were greater than those of the blank control following stimulation with the SP suspension. No significant difference was identified in the levels of IL‑8, ICAM‑1 and IL‑10 secretion between THP‑1 monocytes stimulated by blood‑borne SP (bb‑SP) and sputum‑borne SP (sb‑SP).
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November 2013
Volume 8 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zhang J, Hu D, Wang D, Liu Y, Liu C, Yu L, Qu Y, Luo X, Yang J, Yu J, Yu J, et al: Effects of clinical isolates of Streptococcus pneumoniae on THP-1 human monocytic cells. Mol Med Rep 8: 1570-1574, 2013
APA
Zhang, J., Hu, D., Wang, D., Liu, Y., Liu, C., Yu, L. ... Li, X. (2013). Effects of clinical isolates of Streptococcus pneumoniae on THP-1 human monocytic cells. Molecular Medicine Reports, 8, 1570-1574. https://doi.org/10.3892/mmr.2013.1688
MLA
Zhang, J., Hu, D., Wang, D., Liu, Y., Liu, C., Yu, L., Qu, Y., Luo, X., Yang, J., Yu, J., Liu, S., Li, X."Effects of clinical isolates of Streptococcus pneumoniae on THP-1 human monocytic cells". Molecular Medicine Reports 8.5 (2013): 1570-1574.
Chicago
Zhang, J., Hu, D., Wang, D., Liu, Y., Liu, C., Yu, L., Qu, Y., Luo, X., Yang, J., Yu, J., Liu, S., Li, X."Effects of clinical isolates of Streptococcus pneumoniae on THP-1 human monocytic cells". Molecular Medicine Reports 8, no. 5 (2013): 1570-1574. https://doi.org/10.3892/mmr.2013.1688