Open Access

Identification of differentially expressed inflammatory factors in Wilms' tumors and their association with patient outcomes

  • Authors:
    • Fei Guo
    • Junjie Zhang
    • Lei Wang
    • Wei Zhao
    • Jiekai Yu
    • Shu Zheng
    • Jiaxiang Wang
  • View Affiliations

  • Published online on: May 26, 2017     https://doi.org/10.3892/ol.2017.6261
  • Pages: 687-694
  • Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to identify differentially expressed inflammatory factors observed in Wilms' tumors (WT), and to investigate the association of these factors with clinical stage, pathological type, lymph node metastasis and vascular involvement of WT. Surface‑enhanced laser desorption/ionization‑time of flight mass spectrometry was performed to screen differentially expressed proteins among WT and normal tissue pairs. Upregulated proteins in WT were separated and purified by solid phase extraction and Tricine SDS‑PAGE, respectively. Following in‑gel digestion, the peptide mixture was subjected to liquid chromatography mass spectrometry to identify proteins on the basis of their amino acid sequences. Immunohistochemistry was used to confirm the expression of differentially expressed inflammatory proteins. Of the proteins that were upregulated in WT, two proteins with mass/charge (m/z) ratio of 12,138 and 13,462 were identified as macrophage migration inhibitory factor (MIF) and C‑X‑C motif ligand 7 (CXCL7) chemokine, respectively. The expression of these two proteins was increased in WT compared with adjacent normal tissues and normal renal tissues, and increased with increasing clinical stage. In addition, their expression was significantly increased in patients with unfavorable pathological type, lymph node metastasis and vascular involvement compared with the groups with favorable type, and without lymph node metastasis or vascular involvement (P<0.05). Increased pro‑inflammatory MIF and CXCL7 expression in WT is closely associated with the clinical stage, pathological type, lymph node metastasis and vascular involvement, and may represent biomarkers for the clinical diagnosis of WT.
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July-2017
Volume 14 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Guo F, Zhang J, Wang L, Zhao W, Yu J, Zheng S and Wang J: Identification of differentially expressed inflammatory factors in Wilms' tumors and their association with patient outcomes. Oncol Lett 14: 687-694, 2017
APA
Guo, F., Zhang, J., Wang, L., Zhao, W., Yu, J., Zheng, S., & Wang, J. (2017). Identification of differentially expressed inflammatory factors in Wilms' tumors and their association with patient outcomes. Oncology Letters, 14, 687-694. https://doi.org/10.3892/ol.2017.6261
MLA
Guo, F., Zhang, J., Wang, L., Zhao, W., Yu, J., Zheng, S., Wang, J."Identification of differentially expressed inflammatory factors in Wilms' tumors and their association with patient outcomes". Oncology Letters 14.1 (2017): 687-694.
Chicago
Guo, F., Zhang, J., Wang, L., Zhao, W., Yu, J., Zheng, S., Wang, J."Identification of differentially expressed inflammatory factors in Wilms' tumors and their association with patient outcomes". Oncology Letters 14, no. 1 (2017): 687-694. https://doi.org/10.3892/ol.2017.6261