Correlation of MMP-9 and p53 protein expression with prognosis in metastatic spinal tumor of lung cancer

Zhang,Shuquan; Wu,Minfei; Zhao,Yi; Gu,Rui; Peng,Chuangang; Liu,Jiabei; Zhu,Qingsan; Li,Ye

doi:10.3892/ol.2017.6887

Correlation of MMP-9 and p53 protein expression with prognosis in metastatic spinal tumor of lung cancer

Authors:
- Shuquan Zhang
- Minfei Wu
- Yi Zhao
- Rui Gu
- Chuangang Peng
- Jiabei Liu
- Qingsan Zhu
- Ye Li
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Affiliations: Department of Orthopedics, Tianjin Nankai Hospital, Tianjin 300100, P.R. China, Department of Orthopedics, The Second Hospital of Jilin University, Changchun, Jilin 130041, P.R. China, Department of Surgery, Peking Union Medical College Hospital, Beijing 100032, P.R. China, Department of Orthopedics, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China
Published online on: September 5, 2017 https://doi.org/10.3892/ol.2017.6887
Pages: 5452-5456
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the study was to compare the protein expression of MMP-9 and p53 and examine their correlation with prognosis in lung cancer metastatic spinal tumor. Tissue samples were obtained from 30 cases of para-cancerous tissue (group I), 75 cases of non-metastatic lung cancer tissue (group II) and 100 cases of metastatic spinal tumor tissue of lung cancer (group III). The protein expression of MMP-9 and p53 was detected by immunohistochemistry and was present in all three groups. The positive rate for MMP-9 was 20, 67 and 83%, respectively. There was a significant difference among the three groups (p<0.05). The positive rate for p53 was 16.7, 78.7 and 92%, respectively. There was a highly significant difference among the three groups (p<0.01). There was a positive correlation between the protein expressions of MMP-9 and p53 (Spearman's correlation coefficient r=0.351, p<0.05). The positive or negative expression of the two proteins was statistically significant (p<0.05) for 5-year survival. The expression of MMP-9 and p53 proteins in metastatic spinal tumors of lung cancer showed increasing trends, and the expression of MMP-9 and p53 proteins was significantly higher compared to non-metastatic lung cancer tissue and para-cancerous tissue samples. This likely was associated with the invasion and metastasis of lung cancer to the spine. Survival analysis suggested that the overexpression of p53 and MMP-9 were correlated with poor prognosis.

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1	Wang N and Stamenovic D: Mechanics of vimentin intermediate filaments. J Muscle Res Cell Motil. 23:535–540. 2002. View Article : Google Scholar : PubMed/NCBI
2	Ulirsch J, Fan C, Knafl G, Wu MJ, Coleman B, Perou CM and Swift-Scanlan T: Vimentin DNA methylation predicts survival in breast cancer. Breast Cancer Res Treat. 137:383–396. 2013. View Article : Google Scholar : PubMed/NCBI
3	Kidd ME, Shumaker DK and Ridge KM: The role of vimentin intermediate filaments in the progression of lung cancer. Am J Respir Cell Mol Biol. 50:1–6. 2014.PubMed/NCBI
4	Otsuki S, Inokuchi M, Enjoji M, Ishikawa T, Takagi Y, Kato K, Yamada H, Kojima K and Sugihara K: Vimentin expression is associated with decreased survival in gastric cancer. Oncol Rep. 25:1235–1242. 2011.PubMed/NCBI
5	Schveigert D, Valuckas KP, Kovalcis V, Ulys A, Chvatovic G and Didziapetriene J: Significance of MMP-9 expression and MMP-9 polymorphism in prostate cancer. Tumori. 99:523–529. 2013.PubMed/NCBI
6	Schveigert D, Cicenas S, Bruzas S, Samalavicius NE, Gudleviciene Z and Didziapetriene J: The value of MMP-9 for breast and non-small cell lung cancer patients' survival. Adv Med Sci. 58:73–82. 2013. View Article : Google Scholar : PubMed/NCBI
7	Ureshino RP, Bertoncini CR, Fernandes MJ, Abdalla FM, Porto CS, Hsu YT, Lopes GS and Smaili SS: Alterations in calcium signaling and a decrease in Bcl-2 expression: Possible correlation with apoptosis in aged striatum. J Neurosci Res. 88:438–447. 2010. View Article : Google Scholar : PubMed/NCBI
8	Chernyatina AA, Nicolet S, Aebi U, Herrmann H and Strelkov SV: Atomic structure of the vimentin central α-helical domain and its implications for intermediate filament assembly. Proc Natl Acad Sci USA. 109:13620–13625. 2012. View Article : Google Scholar : PubMed/NCBI
9	Czabotar PE, Lessene G, Strasser A and Adams JM: Control of apoptosis by the BCL-2 protein family: Implications for physiology and therapy. Nat Rev Mol Cell Biol. 15:49–63. 2014. View Article : Google Scholar : PubMed/NCBI
10	Gregory MS, Repp AC, Holhbaum AM, Saff RR, Marshak-Rothstein A and Ksander BR: Membrane Fas ligand activates innate immunity and terminates ocular immune privilege. J Immunol. 169:2727–2735. 2002. View Article : Google Scholar : PubMed/NCBI
11	Alecu M, Coman G and Dănăilă L: High levels of sFas and PBMC apoptosis before and after excision of malignant melanoma - case report. Roum Arch Microbiol Immunol. 61:267–273. 2002.PubMed/NCBI
12	Huang SC, Tang MJ, Hsu KF, Cheng YM and Chou CY: Fas and its ligand, caspases, and bcl-2 expression in gonadotropin-releasing hormone agonist-treated uterine leiomyoma. J Clin Endocrinol Metab. 87:4580–4586. 2002. View Article : Google Scholar : PubMed/NCBI
13	Metser U, Lerman H, Blank A, Lievshitz G, Bokstein F and Even-Sapir E: Malignant involvement of the spine: Assessment by 18F-FDG PET/CT. J Nucl Med. 45:279–284. 2004.PubMed/NCBI
14	Kornblum MB, Wesolowski DP, Fischgrund JS and Herkowitz HN: Computed tomography-guided biopsy of the spine. A review of 103 patients. Spine. 23:81–85. 1998. View Article : Google Scholar : PubMed/NCBI
15	Sucu HK, Ciçek C, Rezanko T, Bezircioğlu H, Erşahin Y, Tunakan M and Minoğlu M: Percutaneous computed-tomography-guided biopsy of the spine: 229 procedures. Joint Bone Spine. 73:532–537. 2006. View Article : Google Scholar : PubMed/NCBI
16	Rades D, Dunst J and Schild SE: The first score predicting overall survival in patients with metastatic spinal cord compression. Cancer. 112:157–161. 2008. View Article : Google Scholar : PubMed/NCBI
17	Masala S, Anselmetti GC, Muto M, Mammucari M, Volpi T and Simonetti G: Percutaneous vertebroplasty relieves pain in metastatic cervical fractures. Clin Orthop Relat Res. 469:715–722. 2011. View Article : Google Scholar : PubMed/NCBI
18	Tseng YY, Lo YL, Chen LH, Lai PL and Yang ST: Percutaneous polymethylmethacrylate vertebroplasty in the treatment of pain induced by metastatic spine tumor. Surg Neurol. 70:78–83. 2008. View Article : Google Scholar
19	Cho DC and Sung JK: Palliative surgery for metastatic thoracic and lumbar tumors using posterolateral transpedicular approach with posterior instrumentation. Surg Neurol. 71:424–433. 2009. View Article : Google Scholar : PubMed/NCBI
20	Sciubba DM, Gokaslan ZL, Black JH III, Simmons O, Suk I, Witham TF, Bydon A and Wolinsky JP: 5-Level spondylectomy for en bloc resection of thoracic chordoma: Case report. Neurosurgery. 69:E248–E256. 2011.