Reversible growth arrest of 3D tumor spheroids stored in oxygen absorber-induced anoxia

  • Authors:
    • Aurélie Gomes
    • Matthieu Defaux
    • Riwanon Michel Lemee
    • Valérie Lobjois
    • Bernard Ducommun
  • View Affiliations

  • Published online on: November 21, 2017     https://doi.org/10.3892/ol.2017.7465
  • Pages:2006-2009
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Abstract

Multicellular tumor spheroids models are of increasing interest in preclinical studies and pharmacological evaluation. However, their storage and transport is often a limitation because it requires adapted and expensive procedures. Here, we propose a very simple method to store 3D spheroids, using a procedure based on oxygen absorber-induced anoxia. We report that oxygen absorbers allow generating an anoxic environment for spheroid storage in culture plates. Oxygen absorber-induced anoxia fully and reversibly arrests spheroid growth for 4 days at 37°C and up to 18 days at 4°C. We then show that the response to etoposide is comparable in spheroids preserved in conditions of absorber-induced anoxia at 4°C and spheroids kept in normoxia at 37°C. These results represent a major improvement that should simplify the storage, transport and use of 3D spheroids.

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February 2018
Volume 15 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

2016 Impact Factor: 1.39
Ranked #68/217 Oncology
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APA
Gomes, A., Defaux, M., Lemee, R.M., Lobjois, V., & Ducommun, B. (2018). Reversible growth arrest of 3D tumor spheroids stored in oxygen absorber-induced anoxia. Oncology Letters, 15, 2006-2009. https://doi.org/10.3892/ol.2017.7465
MLA
Gomes, A., Defaux, M., Lemee, R. M., Lobjois, V., Ducommun, B."Reversible growth arrest of 3D tumor spheroids stored in oxygen absorber-induced anoxia". Oncology Letters 15.2 (2018): 2006-2009.
Chicago
Gomes, A., Defaux, M., Lemee, R. M., Lobjois, V., Ducommun, B."Reversible growth arrest of 3D tumor spheroids stored in oxygen absorber-induced anoxia". Oncology Letters 15, no. 2 (2018): 2006-2009. https://doi.org/10.3892/ol.2017.7465