Dihydromyricetin induces apoptosis in a human choriocarcinoma cell line

Zuo,Yanzhen; Xu,Qian; Lu,Yanjie; Sun,Dayong; Wang,Kang; Lei,Yuntao; Liang,Xiujun; Li,Yuhong

doi:10.3892/ol.2018.9220

Dihydromyricetin induces apoptosis in a human choriocarcinoma cell line

Authors:
- Yanzhen Zuo
- Qian Xu
- Yanjie Lu
- Dayong Sun
- Kang Wang
- Yuntao Lei
- Xiujun Liang
- Yuhong Li
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Affiliations: Department of Pharmacology, Chengde Medical University, Chengde, Hebei 067000, P.R. China, Research Laboratory, Chengde Medical University, Chengde, Hebei 067000, P.R. China, Department of Pathology, Chengde Medical University, Chengde, Hebei 067000, P.R. China, Department of Tumor Radiation and Chemotherapy Center, The Chengde Central Hospital, Chengde, Hebei 067000, P.R. China
Published online on: July 26, 2018 https://doi.org/10.3892/ol.2018.9220
Pages: 4229-4234
Copyright: © Zuo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Choriocarcinoma is a malignant trophoblastic tumor. The development of novel drugs is required to reduce the toxicity of current multi‑agent chemotherapy and to successfully treat chemoresistant cases of the disease. The purpose of the present study was to investigate the effect of dihydromyricetin (DMY) on the human choriocarcinoma cell line, JAr, to identify a novel drug for the treatment of choriocarcinoma. An MTT assay was performed to determine the effects of DMY at different concentrations and for different exposure durations. Flow cytometry and TUNEL assays were performed to detect apoptosis, and western blotting was utilized to investigate the underlying mechanism. The results revealed that DMY significantly inhibited JAr cell viability in a time‑ and dose‑dependent manner. The flow cytometry and TUNEL assays demonstrated that DMY inhibited proliferation by inducing apoptosis. Further analysis by western blotting indicated that the protein expression level of BCL‑2 associated X, associated protein increased, while the protein expression levels of BCL‑2 and pro‑caspase‑3 decreased. These findings suggest that DMY induced apoptosis in human choriocarcinoma JAr cells, through a mitochondrially mediated apoptotic pathway.

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