MicroRNA‑21 promotes proliferation in acute myeloid leukemia by targeting Krüppel‑like factor 5

Li,Chan; Yan,Hua; Yin,Jie; Ma,Jian; Liao,Ang; Yang,Shengyou; Wang,Lijuan; Huang,Yongxiang; Lin,Chon; Dong,Zhiqiang; Yang,Bo; Cao,Ting; Liu,Guo; Wang,Lv

doi:10.3892/ol.2019.10667

MicroRNA‑21 promotes proliferation in acute myeloid leukemia by targeting Krüppel‑like factor 5

Authors:
- Chan Li
- Hua Yan
- Jie Yin
- Jian Ma
- Ang Liao
- Shengyou Yang
- Lijuan Wang
- Yongxiang Huang
- Chon Lin
- Zhiqiang Dong
- Bo Yang
- Ting Cao
- Guo Liu
- Lv Wang
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Affiliations: Department of Hematology and Oncology, Changzhi Medical College Affiliated Heji Hospital, Changzhi, Shanxi 046000, P.R. China, Department of Nuclear Medicine, Guizhou Provincial People's Hospital, Guiyang, Guizhou 550000, P.R. China
Published online on: July 25, 2019 https://doi.org/10.3892/ol.2019.10667
Pages: 3367-3372

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Abstract

Abnormal expression of microRNA (miR)‑21 has been reported in various types of cancers. However, the role and mechanism of miR‑21 remain to be elucidated in acute myeloid leukemia (AML). In the present study, it was observed that miR‑21 was upregulated and Krüppel‑like factor 5 (KLF5) was downregulated in AML cells compared with normal bone marrow cells. Dual luciferase reporter assays revealed that KLF5 was a direct target of miR‑21. Indeed, miR‑21 overexpression resulted in a downregulation of KLF5 expression, while miR‑21 inhibition had the opposite effect in AML cells. In addition, miR‑21 overexpression promoted the proliferation of AML cells in vitro. Notably, using a mouse xenograft model, miR‑21 overexpression was demonstrated to result in enhanced tumor growth and suppressed KLF5 expression in the xenograft tumors in vivo. In conclusion, the present results indicated that miR‑21 promoted proliferation through directly regulating KLF5 expression in AML cells. miR‑21 may thus serve as an oncogene in AML, providing a potential target for AML therapy.

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