Genome-wide DNA methylation analysis in hepatocellular carcinoma

  • Authors:
    • Nobuhisa Yamada
    • Kohichiroh Yasui
    • Osamu Dohi
    • Yasuyuki Gen
    • Akira Tomie
    • Tomoko Kitaichi
    • Naoto Iwai
    • Hironori Mitsuyoshi
    • Yoshio Sumida
    • Michihisa Moriguchi
    • Kanji Yamaguchi
    • Taichiro Nishikawa
    • Atsushi Umemura
    • Yuji Naito
    • Shinji Tanaka
    • Shigeki Arii
    • Yoshito Itoh
  • View Affiliations

  • Published online on: February 11, 2016     https://doi.org/10.3892/or.2016.4619
  • Pages: 2228-2236
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Abstract

Epigenetic changes as well as genetic changes are mechanisms of tumorigenesis. We aimed to identify novel genes that are silenced by DNA hypermethylation in hepatocellular carcinoma (HCC). We screened for genes with promoter DNA hypermethylation using a genome-wide methylation microarray analysis in primary HCC (the discovery set). The microarray analysis revealed that there were 2,670 CpG sites that significantly differed in regards to the methylation level between the tumor and non-tumor liver tissues; 875 were significantly hypermethylated and 1,795 were significantly hypomethylated in the HCC tumors compared to the non‑tumor tissues. Further analyses using methylation-specific PCR, combined with expression analysis, in the validation set of primary HCC showed that, in addition to three known tumor-suppressor genes (APC, CDKN2A, and GSTP1), eight genes (AKR1B1, GRASP, MAP9, NXPE3, RSPH9, SPINT2, STEAP4, and ZNF154) were significantly hypermethylated and downregulated in the HCC tumors compared to the non-tumor liver tissues. Our results suggest that epigenetic silencing of these genes may be associated with HCC.
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April-2016
Volume 35 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Yamada N, Yasui K, Dohi O, Gen Y, Tomie A, Kitaichi T, Iwai N, Mitsuyoshi H, Sumida Y, Moriguchi M, Moriguchi M, et al: Genome-wide DNA methylation analysis in hepatocellular carcinoma. Oncol Rep 35: 2228-2236, 2016
APA
Yamada, N., Yasui, K., Dohi, O., Gen, Y., Tomie, A., Kitaichi, T. ... Itoh, Y. (2016). Genome-wide DNA methylation analysis in hepatocellular carcinoma. Oncology Reports, 35, 2228-2236. https://doi.org/10.3892/or.2016.4619
MLA
Yamada, N., Yasui, K., Dohi, O., Gen, Y., Tomie, A., Kitaichi, T., Iwai, N., Mitsuyoshi, H., Sumida, Y., Moriguchi, M., Yamaguchi, K., Nishikawa, T., Umemura, A., Naito, Y., Tanaka, S., Arii, S., Itoh, Y."Genome-wide DNA methylation analysis in hepatocellular carcinoma". Oncology Reports 35.4 (2016): 2228-2236.
Chicago
Yamada, N., Yasui, K., Dohi, O., Gen, Y., Tomie, A., Kitaichi, T., Iwai, N., Mitsuyoshi, H., Sumida, Y., Moriguchi, M., Yamaguchi, K., Nishikawa, T., Umemura, A., Naito, Y., Tanaka, S., Arii, S., Itoh, Y."Genome-wide DNA methylation analysis in hepatocellular carcinoma". Oncology Reports 35, no. 4 (2016): 2228-2236. https://doi.org/10.3892/or.2016.4619