miR-24 promotes the proliferation, migration and invasion in human tongue squamous cell carcinoma by targeting FBXW7

Zhao,Jingzhu; Hu,Chuanxiang; Chi,Jiadong; Li,Jiansen; Peng,Chen; Yun,Xinwei; Li,Dapeng; Yu,Yang; Li,Yigong; Gao,Ming; Zheng,Xiangqian

doi:10.3892/or.2016.4891

miR-24 promotes the proliferation, migration and invasion in human tongue squamous cell carcinoma by targeting FBXW7

Authors:
- Jingzhu Zhao
- Chuanxiang Hu
- Jiadong Chi
- Jiansen Li
- Chen Peng
- Xinwei Yun
- Dapeng Li
- Yang Yu
- Yigong Li
- Ming Gao
- Xiangqian Zheng
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Affiliations: Department of Thyroid and Neck Tumor, Tianjin Medical University Cancer Institute and Hospital, Key Laboratory of Cancer Prevention and Therapy, Tianjin National Clinical Research Center for Cancer, Tianjin 300060, P.R. China, Department of Anesthesiology, Tianjin Medical University Cancer Institute and Hospital, Key Laboratory of Cancer Prevention and Therapy, Tianjin National Clinical Research Center for Cancer, Tianjin 300060, P.R. China
Published online on: June 22, 2016 https://doi.org/10.3892/or.2016.4891
Pages: 1143-1149

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Abstract

Recent studies suggest that aberrant expression of miR-24 is linked to various human cancers, including tongue squamous cell carcinoma (TSCC). F-box and WD-40 domain protein 7 (FBXW7), a tumor-suppressor gene, is responsible for the degradation of several proto-oncogenes. However, the function and mechanism of miR-24 and FBXW7 in TSCC remains unclear. In the present study, we found that miR-24 was increased in TSCC tissues and cell lines, and that upregulation of miR-24 was associated with advanced clinical stage and a shorter overall survival of TSCC patients. Inhibition of miR-24 significantly suppressed the proliferation, migration and invasion of TSCC cells in vitro. Furthermore, miR-24 repressed FBXW7 expression by directly binding to the 3-untranslated region of FBXW7. Moreover, the suppression of FBXW7 increased the proliferation, migration and invasion of TSCC cells, and the restoration of FBXW7 substantially attenuated the oncogenic effects of miR-24. In conclusion, our results demonstrated that upregulation of miR-24 was associated with tumor progression and poor prognosis in TSCC patients, and that overexpression of miR-24 was correlated with the proliferation, migration and invasion of TSCC cells in vitro, at least partially through regulation of its functional target FBXW7. Thus, miR-24 may serve as a novel potential biomarker for the prognosis of TSCC patients.

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