DEK protein overexpression predicts poor prognosis in pancreatic ductal adenocarcinoma

  • Authors:
    • Jie Sun
    • Fangfang Bi
    • Yang Yang
    • Yuan Zhang
    • Aihua Jin
    • Jinzi Li
    • Zhenhua Lin
  • View Affiliations

  • Published online on: December 8, 2016     https://doi.org/10.3892/or.2016.5302
  • Pages: 857-864
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

DEK, a transcription factor, is involved in mRNA splicing, transcriptional control, cell division and differentiation. Recent studies suggest that DEK overexpression can promote tumorigenesis in a wide range of cancer cell types. However, little is known concerning the status of DEK in pancreatic ductal adenocarcinoma (PDAC). Based on the microarray data from Gene Expression Omnibus (GEO), the expression levels of DEK mRNA in PDAC tissues were significantly higher than levels in the adjacent non-tumor tissues. To explore the clinical features of DEK overexpression in PDAC, 87 PDAC and 52 normal pancreas tissues were selected for immunoenzyme staining of the DEK protein. Localization of the DEK protein was detected in PANC-1 pancreatic cancer cells using immunofluorescence (IF) staining. The correlations between DEK overexpression and the clinical features of PDAC were evaluated using the Chi-squared (χ2) and Fisher's exact tests. The survival rates were calculated by the Kaplan-Meier method, and the relationship between prognostic factors and patient survival was also analyzed by the Cox proportional hazard models. The expression levels of DEK mRNA in PDAC tissues were significantly higher than that in the adjacent non‑tumor tissues. The DEK protein showed a primarily nuclear staining pattern in PDAC. The positive rate of the DEK protein was 52.9% (46/87) in PDAC, which was significantly higher than that in the adjacent normal pancreatic tissues (7.7%, 4/52). DEK overexpression in PDAC was correlated with tumor size, histological grade, tumor‑node‑metastasis (TNM) stage and overall survival (OS) rates. In addition, multivariate analysis demonstrated that DEK overexpression was an independent prognostic factor along with histological grade and TNM stage in patients with PDAC. In conclusion, DEK overexpression is associated with PDAC progression and may be a potential biomarker for poor prognostic evaluation in PDAC.
View Figures
View References

Related Articles

Journal Cover

February-2017
Volume 37 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Sun J, Bi F, Yang Y, Zhang Y, Jin A, Li J and Lin Z: DEK protein overexpression predicts poor prognosis in pancreatic ductal adenocarcinoma. Oncol Rep 37: 857-864, 2017
APA
Sun, J., Bi, F., Yang, Y., Zhang, Y., Jin, A., Li, J., & Lin, Z. (2017). DEK protein overexpression predicts poor prognosis in pancreatic ductal adenocarcinoma. Oncology Reports, 37, 857-864. https://doi.org/10.3892/or.2016.5302
MLA
Sun, J., Bi, F., Yang, Y., Zhang, Y., Jin, A., Li, J., Lin, Z."DEK protein overexpression predicts poor prognosis in pancreatic ductal adenocarcinoma". Oncology Reports 37.2 (2017): 857-864.
Chicago
Sun, J., Bi, F., Yang, Y., Zhang, Y., Jin, A., Li, J., Lin, Z."DEK protein overexpression predicts poor prognosis in pancreatic ductal adenocarcinoma". Oncology Reports 37, no. 2 (2017): 857-864. https://doi.org/10.3892/or.2016.5302