miR-130b regulates the proliferation, invasion and apoptosis of glioma cells via targeting of CYLD

  • Authors:
    • Zhi-Qiang Xiao
    • Teng-Kun Yin
    • Ya-Xing Li
    • Jian-He Zhang
    • Jian-Jun Gu
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  • Published online on: May 19, 2017     https://doi.org/10.3892/or.2017.5651
  • Pages: 167-174
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Abstract

MicroRNAs are short non-coding RNAs that play important roles in gliomas. However, the role of miR-130b in glioma remains unclear. In the present study, miR-130b expression was upregulated in glioma tissues and cell lines. Kaplan-Meier analysis indicated that the upregulation of miR-130b expression correlated with poor prognoses in glioma patients. Multivariate analysis demonstrated that this upregulation and a high-grade classification were independent factors that both predicted poor outcomes for glioma patients. Dual-luciferase assays identified that the cylindromatosis (CYLD) gene is a direct target of miR-130b. Functional studies demonstrated that a miR-130b mimic significantly promoted the growth and invasion of glioma cells, while also inhibiting apoptosis via selective targeting of CYLD, which was enhanced by CYLD-targeted siRNA. In contrast, a miR‑130b inhibitor suppressed these biological behaviors, and this inhibition was reversed by CYLD-targeted siRNA. These data revealed that miR-130b could act as a novel potential diagnostic biomarker for glioma, while also demonstrating the importance of miR‑130b in the cell proliferation and progression of glioma, indicating that it may serve as a useful therapeutic target for glioma.
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July-2017
Volume 38 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Xiao Z, Yin T, Li Y, Zhang J and Gu J: miR-130b regulates the proliferation, invasion and apoptosis of glioma cells via targeting of CYLD. Oncol Rep 38: 167-174, 2017
APA
Xiao, Z., Yin, T., Li, Y., Zhang, J., & Gu, J. (2017). miR-130b regulates the proliferation, invasion and apoptosis of glioma cells via targeting of CYLD. Oncology Reports, 38, 167-174. https://doi.org/10.3892/or.2017.5651
MLA
Xiao, Z., Yin, T., Li, Y., Zhang, J., Gu, J."miR-130b regulates the proliferation, invasion and apoptosis of glioma cells via targeting of CYLD". Oncology Reports 38.1 (2017): 167-174.
Chicago
Xiao, Z., Yin, T., Li, Y., Zhang, J., Gu, J."miR-130b regulates the proliferation, invasion and apoptosis of glioma cells via targeting of CYLD". Oncology Reports 38, no. 1 (2017): 167-174. https://doi.org/10.3892/or.2017.5651