Cigarette smoke and bleomycin-induced pulmonary oxidative stress in rats

  • Authors:
    • Turgut Teke
    • Emin Maden
    • Aysel Kiyici
    • Celalettin Korkmaz
    • Mehmet Gok
    • Faruk Ozer
    • Oktay Imecik
    • Kursat Uzun
  • View Affiliations

  • Published online on: April 17, 2012     https://doi.org/10.3892/etm.2012.550
  • Pages: 121-124
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Abstract

Bleomycin causes pulmonary fibrosis by increasing free oxygen radicals. Cigarette smoke is a strong oxidant which adversely affects pulmonary tissue. We evaluated the effects of cigarette smoke administered with intratracheal bleomycin on pulmonary tissue. We studied 3 groups of rats (n=10): one group received intratracheal saline and served as a control; one received intratracheal bleomycin (IT) (0.5 U/100 g body weight, single dose on the first day), and one group received intratracheal bleomycin (single dose on first day) and tobacco smoke (two times per day) (IT-S). After 4 weeks, the levels of malondialdehyde (MDA) and nitric oxide (NO) and the activities of superoxide dismutase (SOD) and xanthine oxidase (XO) were assayed in the homogenate of the lung tissue samples. The severity of interstitial fibrosis was assessed using the grading system described by Ashcroft. There was more intensive fibrosis in the IT and IT-S than in the control samples (P<0.001). The levels of MDA, NO and activity of XO were significantly increased (P<0.001, <0.002 and <0.002, respectively), and SOD activity (P<0.001) was significantly decreased in the IT group when compared to these values in the control group. The concentration of NO was significantly decreased (P<0.002), and SOD activity was significantly increased (P<0.05) in the lung tissue samples of the IT-S group. Theoretically, the combination of cigarette smoke and bleomycin may have a synergistic effect on oxidative lung injury. In conclusion, we showed that inhalation of cigarette smoke provides protection against oxidative stress in the lung tissue of rats with bleomycin-induced pulmonary fibrosis.

Introduction

Interstitial pulmonary fibrosis is thought to arise as a result of a response to persistent lung injury and inflammation. An oxidant-antioxidant imbalance in the lower respiratory tract has been proposed as a cause of pulmonary fibrosis (1). Cigarette smoking is causally related to the development of desquamative interstitial pneumonia, pulmonary Langerhans cell histiocytosis and idiopathic pulmonary fibrosis (IPF). However, cigarette smoke exposure (CSE) appears to protect against the development of hypersensitivity pneumonitis, a lymphocytic alveolitis provoked by the exposure to organic particles, and sarcoidosis, an inflammatory disorder of unknown etiology (2). The mechanisms by which CSE affects in one or another manner the inflammatory and fibrotic responses in the lung remain to be elucidated. Smoking is a strong oxidant which has a role in the development of pulmonary fibrosis (3).

Bleomycin (BLM) causes pulmonary fibrosis by increasing the free oxygen radical content which causes severe pulmonary damage. An imbalance between oxidants and antioxidants has been proposed in the pathogenesis of BLM-induced lung fibrosis. BLM-induced free oxygen radical production has been shown to stimulate fibroblasts to secrete collagen, and to proliferate and differentiate into myofibroblasts, resulting in a histological appearance that is similar to IPF (4).

The purpose of this study was to evaluate the effect of smoking on oxidative injury in lung tissue of rats with BLM-induced pulmonary fibrosis.

Materials and methods

Adult male Sprague-Dawley rats (body weight, 315–375 g) were maintained at 22°C and 20–50% humidity, with a 12-h light period and supplied with a standard laboratory chow diet and water ad libitum. The animals were assigned to three groups of rats (n=10). One group, the control, received intratracheal normal saline; another group (IT) received intratracheally one dose of BLM (Nippon Kayaku Co., Ltd., Tokyo, Japan) 0.5 U/100 g body weight in 0.3 ml 0.15 M sterile NaCl. The rats in the third group received intratracheal BLM and were later placed in a plastic cage (40 cm x 26 cm x 16 cm) two times a day and exposed to tobacco smoke for 4 weeks. BLM solution was prepared immediately before administration and was administered intraperitoneally directly into the trachea under light anesthesia with sodium pentobarbital (50 mg/kg body weight).

After 4 weeks, the animals were sacrificed at the end of the experiment. The right lungs were removed and were shock frozen immediately after resection and were stored at −80°C until use. Each lung was excised, rinsed in ice-cold physiological saline and homogenized in Tris-HCl buffer, using a tissue homogenizer. The resultant whole tissue homogenate was used for biochemical measurements.

The levels of malondialdehyde (MDA), nitric oxide (NO) and the activity superoxide dismutase (SOD), xanthine oxidase (XO) in the tissue samples were analyzed using the following methods.

The MDA level was estimated by the double-heating method of Wasowicz et al (5). The concentration of MDA was calculated by the absorbance coefficient of the MDA-TBA complex (absorbance coefficient e = 1.56x105 l/mol/cm) and expressed as nmol/g for the lung. Total SOD activity (Cu/Zn and Mn) was determined using the method of Sun et al (6). Activity was expressed as U/mg for the lung.

NO was measured using a colorimetric kit method (cat. no. CM780001, Cayman Chemical Co., USA). The concentration of NO was expressed as μM/g protein. XO activity was determined by the method of Prajda and Weber (7). This activity was expressed as U/g protein.

After sacrifice, each left lung was fixed in a buffered 10% formalin solution for 24 h and embedded in paraffin. Longitudinal sections of the lungs were stained with hematoxylin and eosin (H&E) and were examined for pulmonary fibrosis. Each successive field was individually assessed for the severity of interstitial fibrosis using the semi-quantitative grading system described by Ashcroft et al (8). The entire lung section was reviewed at a magnification of x100. For each of the 30–35 microscopic fields needed to review the section, a score ranging from 0 (normal) to 8 (total fibrosis) was assigned.

Statistical analysis

Results are expressed as the means ± SD. A non-parametric analysis of variance (Kruskal-Wallis method) with post-hoc Bonferroni’s correction was used to determine any significant variance among the five groups. The Mann-Whitney U test was performed for comparison between groups. All analyses were carried out using the SPSS statistical software package, and a probability value of <0.05 was considered to be statistically significant.

Ethical approval

Ethical approval was provided from the Ethics Committee of Meram Medical Faculty, affiliated at the time of the study to Selcuk University, Konya, Turkey.

Results

There was more intensive fibrosis in the IT and IT-S groups than that in the control samples (P<0.001). There was no significant difference between the IT and IT-S groups with respect to fibrosis score (P>0.05).

The results of oxidative stress marker levels of the control, IT and IT-S groups are shown in Table I and Fig. 1. When compared with the control group, oxidative stress was increased significantly in the BLM-administered IT group (P<0.05). When the effect of smoking on oxidative stress in the BLM-treated groups was evaluated, a significant increase in SOD activity and a significant decrease in NO level (determinants of decreased oxidative stress) in the IT-S group compared to the IT group. There was no statistically significant decrease in MDA level and XO activity in the IT-S group when compared to the IT group. In Table II a comparison between groups carried out with the Mann-Whitney U test is shown.

Table I.

Levels of oxidative stress markers in lung tissue.

Table I.

Levels of oxidative stress markers in lung tissue.

GroupLung
SODMDANOXO
C0.3±0.074.5±0.70.18±0.051.39±0.5
IT0.17±0.049.9±2.80.57±0.22.7±0.7
IT-S0.22±0.048.9±2.90.21±0.12.2±0.7

[i] C, control group; IT, intratracheal bleomycin; IT-S, intratracheal bleomycin + tobacco smoke.

Table II.

P-values for comparison between groups.

Table II.

P-values for comparison between groups.

SODMDANOXO
C vs. IT<0.001<0.001<0.002<0.002
IT vs. IT-S<0.05NS<0.002NS

[i] C, control group; IT, intratracheal bleomycin; IT-S, intratracheal bleomycin + tobacco smoke; NS, no significant.

Discussion

The results of the present study indicated increased oxidative stress in the lung tissue of rats that developed after BLM-induced pulmonary fibrosis, and CSE reduced the oxidative injury induced by BLM.

There is limited information on the role of tobacco smoking in the development and outcome of BLM-induced lung fibrosis. The mechanisms by which CSE affects in one way or another the incidence or severity of various interstitial lung diseases are not entirely clear (3). Several studies have aimed to reveal whether a smoking history increases the risk of BLM-induced lung fibrosis. An increased risk in smokers is strongly suggested by Lower et al (9). These authors showed radiographic alterations consistent with BLM-induced lung fibrosis in 55% of smoking patients receiving BLM compared with 0% of non-smokers. An earlier report by Takada et al (10) found that an intratracheal administration of BLM induced fibrotic changes in the lungs of hamsters exposed to cigarette smoke (CS). It was also shown that exposure to CS increased the number of myofibroblasts in alveolar septa of guinea pigs and potentialized pulmonary injury induced by BLM (3).

Various studies have shown a high percentage of ever smokers among individuals with IPF and that CSE is an independent risk factor for the development of IPF (11,12). However, cigarette smoking has only recently been shown to be associated with improved survival in this disease (13). Osanai et al (14) found that tobacco smoke reduced the fibrotic response to BLM. In addition, a number of clinical and experimental studies suggest that CSE reduces the frequency of radiation-induced pneumonitis (15,16).

Theoretically, the combination of CS inhalation and BLM should have a synergistic effect on the development of BLM-induced lung injury. We hypothesized that CS contributes to the development and outcome of BLM-induced lung fibrosis by increasing oxidative stress. Our study tested this hypothesis in rats, and we observed that in contrast to this hypothesis, CSE had no additional effect on the development of fibrosis.

Reduced oxidative stress after CS exposure in rats with BLM-induced lung injury can be explained by hyperoxia and reduced oxidative injury caused by hyperoxia. Firstly, one of the few indicators of improved prognosis in IPF is active cigarette smoking at the time of diagnosis. This phenomenon may be related to the ∼11 mg of carbon monoxide (CO) (or 1–6% CO gas) inhaled with each cigarette (17). Secondly, smokers are now known to have increased airway expression of heme oxygenase-1 (HO-1). HO-1 catalyzes heme degradation to generate CO, biliverdin and free iron. Extensive data suggest that CO can often substitute for HO-1 (18). The toxic properties of CO are well known in the field of pulmonary medicine. The toxic actions of CO relate to its high affinity for hemoglobin (240-fold greater than that of O2). CO replaces O2 rapidly from hemoglobin, causing tissue hypoxia (19). Only recently has it become known that, at very low concentrations, CO participates in many physiological reactions. CO exposure of 100–250 parts per million (ppm) stimulate physiological effects without apparent toxicity. The majority of endogenous CO production originates from active heme metabolism. CSE represents a major source of chronic low level exposure to CO (20).

Inhaled CO initially targets alveolar macrophages and respiratory epithelial cells. CO has been shown to decrease proliferation of fibroblasts (21). CO may limit the generation of ROS, lower the presence of free metal ions and downregulate pro-inflammatory cytokines. Exogenous administration of low concentrations of CO provided protection against oxidative stress in a model of inflammation (22). A low concentration of CO was also found to provide protection from lipopolysaccha-ride-induced injury via directly inhibiting lipid peroxidation and decreasing ROS. Liu et al (23) investigated the effect of CO inhalation on oxidative stress and demonstrated that the MDA level and myeloperoxidase activity increased significantly in rats with lung injury related to lipopolysaccharide; and CO inhalation significantly decreased the MDA and myeloperoxidase accumulation and significantly increased the SOD activity in lungs which indicates that CO repairs oxidative lung injury.

Hyperoxia generates ROS, e.g., superoxide anion and hydrogen peroxide, which can injure the lung (24). Rats exposed to hyperoxia in the presence of a low concentration of CO exhibit less lung injury than control rats exposed to oxygen alone (25). A similar study by Clayton et al (26) demonstrated a statistically significant reduction in pulmonary edema upon exposure to CO and hyperoxia. Since BLM is assumed to induce its toxicity partially by the induction of free radicals, the administration of high inspired oxygen could be hazardous (27). In hamsters treated with BLM and 70% oxygen for 72 h, the mortality was 90% compared with 15% in those animals that received BLM only (28). Zhou et al (21) documented that CO may protect against BLM-induced lung injury in mice. Mice treated with CO and BLM were found to have less severe lung injury than mice treated with BLM alone. In humans, clear data showing an increased risk of BLM-induced lung injury with concomitant oxygen supplementation are lacking; however, because of the data obtained from animal studies, hyperoxia is discouraged during BLM treatment (29).

In conclusion, the inhalation of cigarette smoke, which is similar to the exogenous administration of low concentrations of CO, can provide protection against oxidative stress in the lung tissue of rats with bleomycin-induced pulmonary fibrosis.

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Spandidos Publications style
Teke T, Maden E, Kiyici A, Korkmaz C, Gok M, Ozer F, Imecik O and Uzun K: Cigarette smoke and bleomycin-induced pulmonary oxidative stress in rats. Exp Ther Med 4: 121-124, 2012.
APA
Teke, T., Maden, E., Kiyici, A., Korkmaz, C., Gok, M., Ozer, F. ... Uzun, K. (2012). Cigarette smoke and bleomycin-induced pulmonary oxidative stress in rats. Experimental and Therapeutic Medicine, 4, 121-124. https://doi.org/10.3892/etm.2012.550
MLA
Teke, T., Maden, E., Kiyici, A., Korkmaz, C., Gok, M., Ozer, F., Imecik, O., Uzun, K."Cigarette smoke and bleomycin-induced pulmonary oxidative stress in rats". Experimental and Therapeutic Medicine 4.1 (2012): 121-124.
Chicago
Teke, T., Maden, E., Kiyici, A., Korkmaz, C., Gok, M., Ozer, F., Imecik, O., Uzun, K."Cigarette smoke and bleomycin-induced pulmonary oxidative stress in rats". Experimental and Therapeutic Medicine 4, no. 1 (2012): 121-124. https://doi.org/10.3892/etm.2012.550