Protective effect of vitexin reduces sevoflurane-induced neuronal apoptosis through HIF-1α, VEGF and p38 MAPK signaling pathway in vitro and in newborn rats

Lyu,Zhipai; Cao,Jing; Wang,Ju; Lian,Hongmei

doi:10.3892/etm.2018.5758

Protective effect of vitexin reduces sevoflurane-induced neuronal apoptosis through HIF-1α, VEGF and p38 MAPK signaling pathway in vitro and in newborn rats

Authors:
- Zhipai Lyu
- Jing Cao
- Ju Wang
- Hongmei Lian
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Affiliations: Department of Anesthesia, The Third Affiliated Hospital of Zhengzhou University, Henan, Zhengzhou 450052, P.R. China, Department of Pathology, The Third Affiliated Hospital of Zhengzhou University, Henan, Zhengzhou 450052, P.R. China
Published online on: January 17, 2018 https://doi.org/10.3892/etm.2018.5758
Pages: 3117-3123

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Abstract

Previous studies have demonstrated that Vitexin possesses antihypertensive, anti‑inflammatory and potential anticancer effects. The present study aimed to investigate whether the protective effect of vitexin protects against sevoflurane-induced neuronal apoptosis and the underlying mechanisms of this protective effect. The results demonstrated that Vitexin pretreatment significantly reduced neuronal apoptosis, and inhibited caspase‑3 activity, apoptosis regulator BAX protein expression and malondialdehyde levels in sevoflurane‑induced newborn rats. In addition, Vitexin pretreatment increased superoxide dismutase and glutathione peroxidase activity. Furthermore, it was revealed that treatment with vitexin induced hypoxia inducible factor 1α subunit (HIF‑1α) and vascular endothelial growth factor (VEGF) protein expression, and suppressed phosphorylated‑p38 MAP kinase (p38) protein expression in sevoflurane‑induced newborn rat. Together, the results of the current study suggest that the protective effect of vitexin reduces sevoflurane‑induced neuronal apoptosis through HIF‑1α‑, VEGF‑ and p38‑associated signaling pathways in newborn rats.

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