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Allogeneic adipose-derived stem cell transplantation on knee osteoarthritis rats and its effect on MMP-13 and DDR2

  • Authors:
    • Jianrui Li
    • Xiaobo Zhu
    • Qin Shao
    • Feini Xu
    • Guixin Sun
  • View Affiliations / Copyright

    Affiliations: Department of Traumatology, Shanghai East Hospital Tongji University, Shanghai 200123, P.R. China, Department of Preventive Medicine, Army Medical University, Chongqing 400038, P.R. China, Department of Dermatology, Chongqing Medical University, Chongqing 400016, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 99-104
    |
    Published online on: May 8, 2019
       https://doi.org/10.3892/etm.2019.7554
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Abstract

This study investigated the efficacy of transplantation of allogeneic adipose-derived stem cells (ADMSCs) in rats with knee osteoarthritis (KOA) and the effect on the expression of matrix metalloproteinase 13 (MMP-13) and discoid domain receptor 2 (DDR2). In total, sixty rats were randomly selected. Eleven rats were selected as the blank group. Forty-four rat KOA models were established, and the remaining 5 rats were used for stem cell extraction. The rats were randomly divided into two groups, and the transplantation group was treated with ADMSCs transplantation. The KOA group was intragastrically administered with saline. The expressions of MMP-13 mRNA and DDR2 in rats were detected by RT-qPCR and immunohistochemistry. Correlation analysis was performed in MMP-13 mRNA and DDR2 expression levels in the KOA rats. After treatment, the indexes of Lequesne MG knee joints, MMP-13 mRNA and DDR2 in the transplanted rats were significantly lower than those in the KOA group (P<0.05). In the KOA rats, MMP-13 mRNA and DDR2 was positively correlated (r=0.830, P<0.001). Therefore, the transplantation of ADMSCs has a significant effect on the KOA rats, which can effectively improve the knee joint function of KOA rats and reduce the expression of MMP-13 mRNA and DDR2 in rats, and it is worthy of clinical promotion.
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Copy and paste a formatted citation
Spandidos Publications style
Li J, Zhu X, Shao Q, Xu F and Sun G: Allogeneic adipose-derived stem cell transplantation on knee osteoarthritis rats and its effect on MMP-13 and DDR2. Exp Ther Med 18: 99-104, 2019.
APA
Li, J., Zhu, X., Shao, Q., Xu, F., & Sun, G. (2019). Allogeneic adipose-derived stem cell transplantation on knee osteoarthritis rats and its effect on MMP-13 and DDR2. Experimental and Therapeutic Medicine, 18, 99-104. https://doi.org/10.3892/etm.2019.7554
MLA
Li, J., Zhu, X., Shao, Q., Xu, F., Sun, G."Allogeneic adipose-derived stem cell transplantation on knee osteoarthritis rats and its effect on MMP-13 and DDR2". Experimental and Therapeutic Medicine 18.1 (2019): 99-104.
Chicago
Li, J., Zhu, X., Shao, Q., Xu, F., Sun, G."Allogeneic adipose-derived stem cell transplantation on knee osteoarthritis rats and its effect on MMP-13 and DDR2". Experimental and Therapeutic Medicine 18, no. 1 (2019): 99-104. https://doi.org/10.3892/etm.2019.7554
Copy and paste a formatted citation
x
Spandidos Publications style
Li J, Zhu X, Shao Q, Xu F and Sun G: Allogeneic adipose-derived stem cell transplantation on knee osteoarthritis rats and its effect on MMP-13 and DDR2. Exp Ther Med 18: 99-104, 2019.
APA
Li, J., Zhu, X., Shao, Q., Xu, F., & Sun, G. (2019). Allogeneic adipose-derived stem cell transplantation on knee osteoarthritis rats and its effect on MMP-13 and DDR2. Experimental and Therapeutic Medicine, 18, 99-104. https://doi.org/10.3892/etm.2019.7554
MLA
Li, J., Zhu, X., Shao, Q., Xu, F., Sun, G."Allogeneic adipose-derived stem cell transplantation on knee osteoarthritis rats and its effect on MMP-13 and DDR2". Experimental and Therapeutic Medicine 18.1 (2019): 99-104.
Chicago
Li, J., Zhu, X., Shao, Q., Xu, F., Sun, G."Allogeneic adipose-derived stem cell transplantation on knee osteoarthritis rats and its effect on MMP-13 and DDR2". Experimental and Therapeutic Medicine 18, no. 1 (2019): 99-104. https://doi.org/10.3892/etm.2019.7554
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