Open Access

Resveratrol sensitizes A549 cells to irradiation damage via suppression of store‑operated calcium entry with Orai1 and STIM1 downregulation

  • Authors:
    • Wu Lele
    • Lv Lei
    • Qian Liting
  • View Affiliations

  • Published online on: April 2, 2021     https://doi.org/10.3892/etm.2021.10019
  • Article Number: 587
  • Copyright: © Lele et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Resveratrol is a natural polyphenol with multiple positive biofunctions and was found to have potential as a radiosensitizer with an intricate molecular mechanism. Store‑operated calcium entry (SOCE) is a novel intracellular calcium regulatory pattern that is mainly mediated by iron channels, such as by the stromal interaction molecule (STIM) and calcium release‑activated calcium channel protein (Orai) families. SOCE was recently reported to be suppressed via the downregulation of STIM or Orai families for the promotion of tumor cell death induced by resveratrol. In the present study, resveratrol combined with irradiation treatment were found to induce more evident cell damage compared with irradiation treatment alone, as shown with Cell Counting Kit‑8 assay and mitochondrial membrane potential detection with rhodamine 123. Additionally, resveratrol combined with irradiation treatment decreased the expression of STIM1 and Orai1, while it had no effects on STIM2, Orai2 and Orai3. Moreover, resveratrol combined with irradiation treatment lead to alleviated thapsigargin‑induced SOCE. In addition, overexpression of STIM1 and Orai1 reversed resveratrol‑induced SOCE inhibition and reduced death in A549 cells under irradiation. In summary, the present results revealed that resveratrol can significantly enhance the effect of irradiation damage on lung adenocarcinoma A549 cells, and this effect may be mediated by suppression of SOCE with reduced expression of both STIM1 and Orai1.
View Figures
View References

Related Articles

Journal Cover

June-2021
Volume 21 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Lele W, Lei L and Liting Q: Resveratrol sensitizes A549 cells to irradiation damage via suppression of store‑operated calcium entry with Orai1 and STIM1 downregulation. Exp Ther Med 21: 587, 2021
APA
Lele, W., Lei, L., & Liting, Q. (2021). Resveratrol sensitizes A549 cells to irradiation damage via suppression of store‑operated calcium entry with Orai1 and STIM1 downregulation. Experimental and Therapeutic Medicine, 21, 587. https://doi.org/10.3892/etm.2021.10019
MLA
Lele, W., Lei, L., Liting, Q."Resveratrol sensitizes A549 cells to irradiation damage via suppression of store‑operated calcium entry with Orai1 and STIM1 downregulation". Experimental and Therapeutic Medicine 21.6 (2021): 587.
Chicago
Lele, W., Lei, L., Liting, Q."Resveratrol sensitizes A549 cells to irradiation damage via suppression of store‑operated calcium entry with Orai1 and STIM1 downregulation". Experimental and Therapeutic Medicine 21, no. 6 (2021): 587. https://doi.org/10.3892/etm.2021.10019