Clinical significance of serum matrix metalloproteinase 9 and tissue inhibitor of metalloproteinase 1 in the first phase of burn trauma evolution

Stanciu,Adina Elena; Zamfir‑Chiru‑Anton,Adina; Stanciu,Marcel Marian; Gherghe,Mirela; Hainarosie,Razvan; Furtunescu,Florentina Ligia; Gheorghe,Dan Cristian

doi:10.3892/etm.2021.10547

Clinical significance of serum matrix metalloproteinase 9 and tissue inhibitor of metalloproteinase 1 in the first phase of burn trauma evolution

Authors:
- Adina Elena Stanciu
- Adina Zamfir‑Chiru‑Anton
- Marcel Marian Stanciu
- Mirela Gherghe
- Razvan Hainarosie
- Florentina Ligia Furtunescu
- Dan Cristian Gheorghe
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Affiliations: Department of Carcinogenesis and Molecular Biology, ‘Prof. Dr. Alexandru Trestioreanu’ Institute of Oncology, 022328 Bucharest, Romania, ENT Department, ‘Grigore Alexandrescu’ Children's Emergency Hospital, 011743 Bucharest, Romania, Electrical Engineering Faculty, ‘Politehnica’ University, 060042, Bucharest, Romania, Department of Nuclear Medicine, ‘Prof. Dr. Alexandru Trestioreanu’ Institute of Oncology, 022328 Bucharest, Romania, ENT Department, ‘Prof. Dr. Dorin Hociota’ Institute of Phonoaudiology and Functional ENT and Cervicofacial Surgery, 050751 Bucharest, Romania, Department of Complementary Sciences, ‘Carol Davila’ University of Medicine and Pharmacy, 050463 Bucharest, Romania, ENT Department, ‘Carol Davila’ University of Medicine and Pharmacy, 050463 Bucharest, Romania
Published online on: August 3, 2021 https://doi.org/10.3892/etm.2021.10547
Article Number: 1113

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Abstract

No prospective study has specifically examined the serum levels of matrix metalloproteinase‑9 (MMP‑9) and tissue inhibitor of metalloproteinase‑1 (TIMP‑1) in the early shock phase of burn‑injured patients. Thus, we aimed to detect early changes, activity dynamics, and the predictive value of MMP‑9, TIMP‑1, and the MMP‑9/TIMP‑1 ratio to better understand the early repair mechanisms for the development of future therapies for patients with thermal burns. Twenty‑five patients with a total body surface area (TBSA) affected by burn <25%, and 30 healthy subjects were enrolled in the study. Serum levels of MMP‑9 and TIMP‑1 were determined by the ELISA method. Our results showed that MMP‑9 concentrations increased immediately after injury and remained on a plateau. In contrast, TIMP‑1 showed an upward trend throughout the 7‑day study period, and the time course of the MMP‑9/TIMP‑1 ratio followed the inverse dynamics of TIMP‑1. Analysis of the areas under the receiver operating characteristic (ROC) curves (AUC) showed that patients with burn wounds tended to have a MMP‑9 value higher than 421.5 ng/ml (AUC=0.979), TIMP‑1 value higher than 231.6 ng/ml (AUC=0.908), and MMP‑9/TIMP‑1 ratio higher than 2.31 (AUC=0.959) (P<0.001). Our findings suggest that although the variations in the two biomarkers were different regarding the time of the initial insult, their ratio is a specific and sensitive indicator of burn evolutivity in patients with a TBSA affected by a burn <25%.

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