Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
November-2021 Volume 22 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
November-2021 Volume 22 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

hsa‑miR‑15b‑5p regulates the proliferation and apoptosis of human vascular smooth muscle cells by targeting the ACSS2/PTGS2 axis

  • Authors:
    • Shujie Gan
    • Jieqi Mao
    • Yuqin Pan
    • Jingdong Tang
    • Zhengjun Qiu
  • View Affiliations / Copyright

    Affiliations: Department of Vascular Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, P.R. China, Department of Nursing, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, P.R. China, Department of Vascular Surgery, Shanghai Pudong Hospital Affiliated to Fudan University, Shanghai 201399, P.R. China
    Copyright: © Gan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 1208
    |
    Published online on: August 24, 2021
       https://doi.org/10.3892/etm.2021.10642
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

A previous bioinformatic analysis from our group predicted that the interaction of microRNA (miRNA/miR)‑15b with the acyl‑CoA synthetase short chain family member 2 (ACSS2) gene was important for the development of abdominal aortic aneurysm (AAA). Apoptosis of aortic vascular smooth muscle cells (VSMCs) is a pathological feature of AAA. The present study aimed to explain the roles of miR‑15b/ACSS2 in AAA by exploring their effects on the proliferation and apoptosis of aortic VSMCs. Human aortic VSMCs (T/G HA‑VSMC cell line) were divided into six groups and were transfected with miR‑15b‑5p mimics, mimic negative control (NC), miR‑15b‑5p inhibitors, inhibitor NC, miR‑15b‑5p mimics+pcDNA3.1 and miR‑15b‑5p mimics+ACSS2‑overexpessing vector. CCK‑8 assay was used to determine cell proliferation. Annexin V‑FITC/PI staining and flow cytometry assays were used to measure cell apoptosis. Dual‑luciferase reporter assays were used to confirm the targeted relationship between miR‑15b‑5p and ACSS2. Reverse transcription‑quantitative PCR and/or western blotting were used to examine the expression levels of miR‑15b‑5p, ACSS2 and prostaglandin‑endoperoxide synthase 2 (PTGS2). Following transfection of T/G HA‑VSMCs with mimics and inhibitors to respectively upregulate and downregulate miR‑15b‑5p, the results demonstrated that overexpression of miR‑15b‑5p inhibited cell proliferation and promoted cell apoptosis; silencing of miR‑15b‑5p obtained the opposite results. ACSS2 may be a direct target of miR‑15b‑5p, since the luciferase activity of a ACSS2 wild‑type vector, but not that of a ACSS2 mutant reporter, was significantly inhibited by miR‑15b‑5p mimics compared with controls. Additionally, the expression levels of ACSS2 and its downstream gene PTGS2 were significantly reduced or increased following transfection with miR‑15b‑5p mimics or inhibitors, respectively. Furthermore, overexpression of ACSS2 reversed the antiproliferative and proapoptotic effects of miR‑15b‑5p mimics by blocking the production of PTGS2 protein. In conclusion, miR‑15b‑5p may promote the apoptosis and inhibit the proliferation of aortic VSMCs via targeting the ACSS2/PTGS2 axis. The present study provided preliminary evidence indicating that the miR‑15b‑5p/ACSS2/PTGS2 axis may be a potential target for the treatment of AAA.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Kent KC: Clinical practice. Abdominal aortic aneurysms. N Engl J Med. 371:2101–2108. 2014.PubMed/NCBI View Article : Google Scholar

2 

Dereziński TL, Fórmankiewicz B, Migdalski A, Brazis P, Jakubowski G, Woda Ł and Jawień A: The prevalence of abdominal aortic aneurysms in the rural/urban population in central Poland-Gniewkowo aortic study. Kardiol Pol. 75:705–710. 2017.PubMed/NCBI View Article : Google Scholar

3 

Stuntz M: Modeling the burden of abdominal aortic aneurysm in the USA in 2013. Cardiology. 135:127–131. 2016.PubMed/NCBI View Article : Google Scholar

4 

Kühnl A, Erk A, Trenner M, Salvermoser M, Schmid V and Eckstein HH: Incidence, treatment and mortality in patients with abdominal aortic aneurysms. Dtsch Arztebl Int. 114:391–398. 2017.PubMed/NCBI View Article : Google Scholar

5 

Lederle FA, Kyriakides TC, Stroupe KT, Freischlag JA, Padberg FT Jr, Matsumura JS, Huo Z and Johnson GR: OVER Veterans Affairs Cooperative Study Group. Open versus endovascular repair of abdominal aortic aneurysm. N Engl J Med. 380:2126–2135. 2019.PubMed/NCBI View Article : Google Scholar

6 

Thompson RW, Liao S and Curci JA: Vascular smooth muscle cell apoptosis in abdominal aortic aneurysms. Coron Artery Dis. 8:623–631. 1997.PubMed/NCBI View Article : Google Scholar

7 

Liang B, Che J, Zhao H, Zhang Z and Shi G: MiR-195 promotes abdominal aortic aneurysm media remodeling by targeting Smad3. Cardiovasc Ther. 35(e12286)2017.PubMed/NCBI View Article : Google Scholar

8 

Zhao F, Chen T and Jiang N: CDR1as/miR-7/CKAP4 axis contributes to the pathogenesis of abdominal aortic aneurysm by regulating the proliferation and apoptosis of primary vascular smooth muscle cells. Exp Ther Med. 19:3760–3766. 2020.PubMed/NCBI View Article : Google Scholar

9 

Cao X, Cai Z, Liu J, Zhao Y, Wang X, Li X and Xia H: miRNA-504 inhibits p53-dependent vascular smooth muscle cell apoptosis and may prevent aneurysm formation. Mol Med Rep. 16:2570–2578. 2017.PubMed/NCBI View Article : Google Scholar

10 

Zhang Y, Liu Z, Zhou M and Liu C: MicroRNA-129-5p inhibits vascular smooth muscle cell proliferation by targeting Wnt5a. Exp Ther Med. 12:2651–2656. 2016.PubMed/NCBI View Article : Google Scholar

11 

Zhao L, Ouyang Y, Bai Y, Gong J and Liao H: miR-155-5p inhibits the viability of vascular smooth muscle cell via targeting FOS and ZIC3 to promote aneurysm formation. Eur J Pharmacol. 853:145–152. 2019.PubMed/NCBI View Article : Google Scholar

12 

Yue J, Zhu T, Yang J, Si Y, Xu X, Fang Y and Fu W: CircCBFB-mediated miR-28-5p facilitates abdominal aortic aneurysm via LYPD3 and GRIA4. Life Sci. 253(117533)2020.PubMed/NCBI View Article : Google Scholar

13 

Maegdefessel L, Azuma J, Toh R, Deng A, Merk DR, Raiesdana A, Leeper NJ, Raaz U, Schoelmerich AM, McConnell MV, et al: MicroRNA-21 blocks abdominal aortic aneurysm development and nicotine-augmented expansion. Sci Transl Med. 4(122ra22)2012.PubMed/NCBI View Article : Google Scholar

14 

Peng J, He X, Zhang L and Liu P: MicroRNA-26a protects vascular smooth muscle cells against H2O2-induced injury through activation of the PTEN/AKT/mTOR pathway. Int J Mol Med. 42:1367–1378. 2018.PubMed/NCBI View Article : Google Scholar

15 

Shen G, Sun Q, Yao Y, Li S, Liu G, Yuan C, Li H, Xu Y and Wang H: Role of ADAM9 and miR-126 in the development of abdominal aortic aneurysm. Atherosclerosis. 297:47–54. 2020.PubMed/NCBI View Article : Google Scholar

16 

Gan S, Pan Y and Mao J: miR-30a-GNG2 and miR-15b-ACSS2 interaction pairs may be potentially crucial for development of abdominal aortic aneurysm by influencing inflammation. DNA Cell Biol. 38:1540–1556. 2019.PubMed/NCBI View Article : Google Scholar

17 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

18 

Agarwal V, Bell GW, Nam JW and Bartel DP: Predicting effective microRNA target sites in mammalian mRNAs. Elife. 4(e05005)2015.PubMed/NCBI View Article : Google Scholar

19 

Zhang Z, Zou G, Chen X, Lu W, Liu J, Zhai S and Qiao G: Knockdown of lncRNA PVT1 inhibits vascular smooth muscle cell apoptosis and extracellular matrix disruption in a murine abdominal aortic aneurysm model. Mol Cells. 42:218–227. 2019.PubMed/NCBI View Article : Google Scholar

20 

Xue M, Li G, Li D, Wang Z, Mi L, Da J and Jin X: Up-regulated MCPIP1 in abdominal aortic aneurysm is associated with vascular smooth muscle cell apoptosis and MMPs production. Biosci Rep. 39(BSR20191252)2019.PubMed/NCBI View Article : Google Scholar

21 

Dong Y, Zhang N, Zhao S, Chen X, Li F and Tao X: miR-221-3p and miR-15b-5p promote cell proliferation and invasion by targeting Axin2 in liver cancer. Oncol Lett. 18:6491–6500. 2019.PubMed/NCBI View Article : Google Scholar

22 

Zhao C, Li Y, Chen G, Wang F, Shen Z and Zhou R: Overexpression of miR-15b-5p promotes gastric cancer metastasis by regulating PAQR3. Oncol Rep. 38:352–358. 2017.PubMed/NCBI View Article : Google Scholar

23 

Wu B, Liu G, Jin Y, Yang T, Zhang D, Ding L, Zhou F, Pan Y and Wei Y: miR-15b-5p promotes growth and metastasis in breast cancer by targeting HPSE2. Front Oncol. 10(108)2020.PubMed/NCBI View Article : Google Scholar

24 

Sun Y, Gao Y, Song T, Yu C, Nie Z and Wang X: MicroRNA-15b participates in the development of peripheral arterial disease by modulating the growth of vascular smooth muscle cells. Exp Ther Med. 18:77–84. 2019.PubMed/NCBI View Article : Google Scholar

25 

Chen R, Sheng L, Zhang HJ, Ji M and Qian WQ: miR-15b-5p facilitates the tumorigenicity by targeting RECK and predicts tumour recurrence in prostate cancer. J Cell Mol Med. 22:1855–1863. 2018.PubMed/NCBI View Article : Google Scholar

26 

Hur H, Kim YB, Ham IH and Lee D: Loss of ACSS2 expression predicts poor prognosis in patients with gastric cancer. J Surg Oncol. 112:585–591. 2015.PubMed/NCBI View Article : Google Scholar

27 

Bae JM, Kim JH, Oh HJ, Park HE, Lee TH, Cho NY and Kang GH: Downregulation of acetyl-CoA synthetase 2 is a metabolic hallmark of tumor progression and aggressiveness in colorectal carcinoma. Mod Pathol. 30:267–277. 2017.PubMed/NCBI View Article : Google Scholar

28 

Sun L, Kong Y, Cao M, Zhou H, Li H, Cui Y, Fang F, Zhang W, Li J, Zhu X, et al: Decreased expression of acetyl-CoA synthase 2 promotes metastasis and predicts poor prognosis in hepatocellular carcinoma. Cancer Sci. 108:1338–1346. 2017.PubMed/NCBI View Article : Google Scholar

29 

Yao L, Guo X and Gui Y: Acetyl-CoA synthetase 2 promotes cell migration and invasion of renal cell carcinoma by upregulating lysosomal-associated membrane protein 1 expression. Cell Physiol Biochem. 45:984–992. 2018.PubMed/NCBI View Article : Google Scholar

30 

Zhang S, He J, Jia Z, Yan Z and Yang J: Acetyl-CoA synthetase 2 enhances tumorigenesis and is indicative of a poor prognosis for patients with renal cell carcinoma. Urol Oncol. 36:243.e9–243.e20. 2018.PubMed/NCBI View Article : Google Scholar

31 

Mi L, Zhou Y, Wu D, Tao Q, Wang X, Zhu H, Gao X, Wang J, Ling R, Deng J, et al: ACSS2/AMPK/PCNA pathway-driven proliferation and chemoresistance of esophageal squamous carcinoma cells under nutrient stress. Mol Med Rep. 20:5286–5296. 2019.PubMed/NCBI View Article : Google Scholar

32 

Huang Z, Zhang M, Plec AA, Estill SJ, Cai L, Repa JJ, McKnight SL and Tu BP: ACSS2 promotes systemic fat storage and utilization through selective regulation of genes involved in lipid metabolism. Proc Natl Acad Sci USA. 115:E9499–E9506. 2018.PubMed/NCBI View Article : Google Scholar

33 

Lee MY, Yeon A, Shahid M, Cho E, Sairam V, Figlin R, Kim KH and Kim J: Reprogrammed lipid metabolism in bladder cancer with cisplatin resistance. Oncotarget. 9:13231–13243. 2018.PubMed/NCBI View Article : Google Scholar

34 

Xu H, Luo J, Ma G, Zhang X, Yao D, Li M and Loor JJ: Acyl-CoA synthetase short-chain family member 2 (ACSS2) is regulated by SREBP-1 and plays a role in fatty acid synthesis in caprine mammary epithelial cells. J Cell Physiol. 233:1005–1016. 2018.PubMed/NCBI View Article : Google Scholar

35 

Chapple KS, Parry DJ, McKenzie S, MacLennan KA, Jones P and Scott DJ: Cyclooxygenase-2 expression and its association with increased angiogenesis in human abdominal aortic aneurysms. Ann Vasc Surg. 21:61–66. 2007.PubMed/NCBI View Article : Google Scholar

36 

Ghoshal S and Loftin CD: Cyclooxygenase-2 inhibition attenuates abdominal aortic aneurysm progression in hyperlipidemic mice. PLoS One. 7(e44369)2012.PubMed/NCBI View Article : Google Scholar

37 

King VL, Trivedi DB, Gitlin JM and Loftin CD: Selective cyclooxygenase-2 inhibition with celecoxib decreases angiotensin II-induced abdominal aortic aneurysm formation in mice. Arterioscler Thromb Vasc Biol. 26:1137–1143. 2006.PubMed/NCBI View Article : Google Scholar

38 

Mukherjee K, Gitlin JM and Loftin CD: Effectiveness of cyclooxygenase-2 inhibition in limiting abdominal aortic aneurysm progression in mice correlates with a differentiated smooth muscle cell phenotype. J Cardiovasc Pharmacol. 60:520–529. 2012.PubMed/NCBI View Article : Google Scholar

39 

Gitlin JM, Trivedi DB, Langenbach R and Loftin CD: Genetic deficiency of cyclooxygenase-2 attenuates abdominal aortic aneurysm formation in mice. Cardiovasc Res. 73:227–236. 2007.PubMed/NCBI View Article : Google Scholar

40 

Walton LJ, Franklin IJ, Bayston T, Brown LC, Greenhalgh RM, Taylor GW and Powell JT: Inhibition of prostaglandin E2 synthesis in abdominal aortic aneurysms: Implications for smooth muscle cell viability, inflammatory processes, and the expansion of abdominal aortic aneurysms. Circulation. 100:48–54. 1999.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Gan S, Mao J, Pan Y, Tang J and Qiu Z: hsa‑miR‑15b‑5p regulates the proliferation and apoptosis of human vascular smooth muscle cells by targeting the ACSS2/PTGS2 axis. Exp Ther Med 22: 1208, 2021.
APA
Gan, S., Mao, J., Pan, Y., Tang, J., & Qiu, Z. (2021). hsa‑miR‑15b‑5p regulates the proliferation and apoptosis of human vascular smooth muscle cells by targeting the ACSS2/PTGS2 axis. Experimental and Therapeutic Medicine, 22, 1208. https://doi.org/10.3892/etm.2021.10642
MLA
Gan, S., Mao, J., Pan, Y., Tang, J., Qiu, Z."hsa‑miR‑15b‑5p regulates the proliferation and apoptosis of human vascular smooth muscle cells by targeting the ACSS2/PTGS2 axis". Experimental and Therapeutic Medicine 22.5 (2021): 1208.
Chicago
Gan, S., Mao, J., Pan, Y., Tang, J., Qiu, Z."hsa‑miR‑15b‑5p regulates the proliferation and apoptosis of human vascular smooth muscle cells by targeting the ACSS2/PTGS2 axis". Experimental and Therapeutic Medicine 22, no. 5 (2021): 1208. https://doi.org/10.3892/etm.2021.10642
Copy and paste a formatted citation
x
Spandidos Publications style
Gan S, Mao J, Pan Y, Tang J and Qiu Z: hsa‑miR‑15b‑5p regulates the proliferation and apoptosis of human vascular smooth muscle cells by targeting the ACSS2/PTGS2 axis. Exp Ther Med 22: 1208, 2021.
APA
Gan, S., Mao, J., Pan, Y., Tang, J., & Qiu, Z. (2021). hsa‑miR‑15b‑5p regulates the proliferation and apoptosis of human vascular smooth muscle cells by targeting the ACSS2/PTGS2 axis. Experimental and Therapeutic Medicine, 22, 1208. https://doi.org/10.3892/etm.2021.10642
MLA
Gan, S., Mao, J., Pan, Y., Tang, J., Qiu, Z."hsa‑miR‑15b‑5p regulates the proliferation and apoptosis of human vascular smooth muscle cells by targeting the ACSS2/PTGS2 axis". Experimental and Therapeutic Medicine 22.5 (2021): 1208.
Chicago
Gan, S., Mao, J., Pan, Y., Tang, J., Qiu, Z."hsa‑miR‑15b‑5p regulates the proliferation and apoptosis of human vascular smooth muscle cells by targeting the ACSS2/PTGS2 axis". Experimental and Therapeutic Medicine 22, no. 5 (2021): 1208. https://doi.org/10.3892/etm.2021.10642
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team