Accelerated lipid peroxidation in a rat model of gentamicin nephrotoxicity

  • Authors:
    • Anamaria Magdalena Tomşa
    • Andreea Liana Răchişan
    • Stanca Lucia Pandrea
    • Andreea Benea
    • Ana Uifălean
    • Alina Elena Parvu
    • Lia Monica Junie
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  • Published online on: August 26, 2021     https://doi.org/10.3892/etm.2021.10652
  • Article Number: 1218
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Abstract

Kidney disease represents a burden for the health care system worldwide. As the prevalence continues to rise, discovering new biomarkers of early kidney damage has become crucial. Oxidative stress (OS) represents one of the main factors involved in the early stages of many syndromes leading to kidney damage. Therefore, it must be studied in detail. To date, many studies have focused on OS in advanced stages of acute kidney injury (AKI), with great success. The aim of the present study was to ascertain whether even mild renal function impairment can be linked to specific systemic markers of OS and systemic antioxidants in order to pinpoint certain biomarkers for early kidney damage. We used male rats (Rattus norvegicus) in which we induced kidney damage by injecting gentamicin for 7 days. Blood was collected 24 h after the last dose of gentamicin. Urea, creatinine, 3‑nitrotyrosine (3‑NT), nitric oxide (NO), malondialdehyde (MDA), thiols (TS), total oxidative stress (TOS), and interferon‑γ (IFN‑γ) were determined. In addition, for the antioxidant status we measured total antioxidant capacity (TAC) and interleukin‑10 (IL‑10). Our results demonstrated that the rats had mild renal impairment consistent with a pre‑AKI stage due to the nephrotoxic effect of gentamicin. However, TOS, MDA and NO were significantly higher in the gentamicin group compared to the control group. In addition, TAC was higher in the control group. Hence, OS markers reach higher levels and may potentially be used as markers of kidney damage even in cases of mild renal function impairment.
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November-2021
Volume 22 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Tomşa AM, Răchişan AL, Pandrea SL, Benea A, Uifălean A, Parvu AE and Junie LM: Accelerated lipid peroxidation in a rat model of gentamicin nephrotoxicity. Exp Ther Med 22: 1218, 2021
APA
Tomşa, A.M., Răchişan, A.L., Pandrea, S.L., Benea, A., Uifălean, A., Parvu, A.E., & Junie, L.M. (2021). Accelerated lipid peroxidation in a rat model of gentamicin nephrotoxicity. Experimental and Therapeutic Medicine, 22, 1218. https://doi.org/10.3892/etm.2021.10652
MLA
Tomşa, A. M., Răchişan, A. L., Pandrea, S. L., Benea, A., Uifălean, A., Parvu, A. E., Junie, L. M."Accelerated lipid peroxidation in a rat model of gentamicin nephrotoxicity". Experimental and Therapeutic Medicine 22.5 (2021): 1218.
Chicago
Tomşa, A. M., Răchişan, A. L., Pandrea, S. L., Benea, A., Uifălean, A., Parvu, A. E., Junie, L. M."Accelerated lipid peroxidation in a rat model of gentamicin nephrotoxicity". Experimental and Therapeutic Medicine 22, no. 5 (2021): 1218. https://doi.org/10.3892/etm.2021.10652