Open Access

Rapid detection of human heart‑type fatty acid‑binding protein in human plasma and blood using a colloidal gold‑based lateral flow immunoassay

  • Authors:
    • Yang-Guang Ren
    • Mei-Chen Liu
    • Meng-Zhen Ji
    • Chen Chen
    • Hang-Zhan Hu
    • Zi-Xuan Wang
    • Pin-Qian Yu
    • Jia-Ming Shang
    • Qian-Wen Zhou
    • Ning-Ya Tao
    • Ya-Fei Guo
    • Yan-Jiao Lu
    • Zhi-Zeng Wang
  • View Affiliations

  • Published online on: August 31, 2021     https://doi.org/10.3892/etm.2021.10673
  • Article Number: 1238
  • Copyright: © Ren et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The incidence of acute myocardial infarction (AMI) is currently increasing. Early detection is important for the treatment and prognosis of patients with AMI. Heart‑type fatty acid‑binding protein (H‑FABP) may be used as an early marker of AMI due to its high sensitivity, specificity and prognostic value. Therefore, in the present study, H‑FABP was used as a biomarker in a double‑antibody sandwich method and colloidal gold‑based lateral flow immunoassay to develop a rapid detection kit for H‑FABP with a processing time of only 5 min. The sensitivity of the kit in plasma and whole blood was 1 ng/ml and this method had good specificity, exhibiting no cross‑reaction with cardiac troponin I, myoglobin or creatine kinase‑Mb. The kits had good shelf life and stability, as they were able to be stored at 40˚C for 30 days. A total of 12 clinical samples were collected for detection and the coincidence rate with the ELISA method was up to 91.67%. Therefore, the present study provided a simple, rapid and economical early‑detection in‑home testing kit.
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November-2021
Volume 22 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Ren Y, Liu M, Ji M, Chen C, Hu H, Wang Z, Yu P, Shang J, Zhou Q, Tao N, Tao N, et al: Rapid detection of human heart‑type fatty acid‑binding protein in human plasma and blood using a colloidal gold‑based lateral flow immunoassay. Exp Ther Med 22: 1238, 2021.
APA
Ren, Y., Liu, M., Ji, M., Chen, C., Hu, H., Wang, Z. ... Wang, Z. (2021). Rapid detection of human heart‑type fatty acid‑binding protein in human plasma and blood using a colloidal gold‑based lateral flow immunoassay. Experimental and Therapeutic Medicine, 22, 1238. https://doi.org/10.3892/etm.2021.10673
MLA
Ren, Y., Liu, M., Ji, M., Chen, C., Hu, H., Wang, Z., Yu, P., Shang, J., Zhou, Q., Tao, N., Guo, Y., Lu, Y., Wang, Z."Rapid detection of human heart‑type fatty acid‑binding protein in human plasma and blood using a colloidal gold‑based lateral flow immunoassay". Experimental and Therapeutic Medicine 22.5 (2021): 1238.
Chicago
Ren, Y., Liu, M., Ji, M., Chen, C., Hu, H., Wang, Z., Yu, P., Shang, J., Zhou, Q., Tao, N., Guo, Y., Lu, Y., Wang, Z."Rapid detection of human heart‑type fatty acid‑binding protein in human plasma and blood using a colloidal gold‑based lateral flow immunoassay". Experimental and Therapeutic Medicine 22, no. 5 (2021): 1238. https://doi.org/10.3892/etm.2021.10673