Quercetin suppresses inflammatory cytokine production in rheumatoid arthritis fibroblast‑like synoviocytes

Sun,Hai-Tao; Li,Jian-Peng; Qian,Wei-Qing; Yin,Meng-Fan; Yin,Hong; Huang,Gui-Cheng

doi:10.3892/etm.2021.10695

Quercetin suppresses inflammatory cytokine production in rheumatoid arthritis fibroblast‑like synoviocytes

Authors:
- Hai-Tao Sun
- Jian-Peng Li
- Wei-Qing Qian
- Meng-Fan Yin
- Hong Yin
- Gui-Cheng Huang
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Affiliations: Laboratory of New Techniques of Restoration and Reconstruction of Orthopedics and Traumatology, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, P.R. China, Department of Orthopedics, Tianjin Medical University, Tianjin 300070, P.R. China, Department of Orthopedics, The Third Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210001, P.R. China, Department of Orthopedics, Tianjin Fifth Central Hospital, Tianjin 300450, P.R. China, Laboratory of New Techniques of Restoration and Reconstruction of Orthopedics and Traumatology, The First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, P.R. China
Published online on: September 6, 2021 https://doi.org/10.3892/etm.2021.10695
Article Number: 1260
Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Rheumatoid arthritis (RA) is a chronic, progressive and systemic autoimmune disease mainly characterized by symmetric multijoint synovitis. Quercetin has anti‑inflammatory, anti‑oxidation and immune regulation activities, and therefore shows high medicinal value. The present study aimed to observe the effect of quercetin on fibroblast‑like synoviocytes (FLSs) in RA. Rheumatoid arthritis fibroblast‑like synoviocytes (RAFLSs) were pretreated with 50 nmol/l quercetin for 2 h and were then stimulated using TNF‑α for 24 h for subsequent experiments. RAFLSs were transfected with short interfering (si)‑X‑inactive specific transcript (XIST), microRNA (miR)‑485 mimic, miR‑485 inhibitor or si‑PSMB8 or combination. ELISA, PCR and western blotting was used to evaluate the effect of quercetin on RAFLSs treated with TNF‑α. It was revealed that quercetin inhibited the production of inflammatory cytokines and the expression of XIST in RAFLSs induced by TNF‑α. Bioinformatics analysis indicated that XIST acted as a sponge for miR‑485 and that proteasome subunit β type‑8 (PSMB8) was a direct target of miR‑485. Moreover, PSMB8 functioned as a suppressor in inflammatory cytokine production of RAFLSs induced by TNF‑α. Overall, quercetin was observed to inhibit the production of inflammatory cytokines and the expression of XIST in RAFLSs induced by TNF‑α. Moreover, XIST‑silencing could suppress the inflammatory reaction by sponging miR‑485 in cells treated with TNF‑α. Altogether, quercetin could suppress the development of RA in vitro.

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