Ubisol Coenzyme Q10 promotes mitochondrial biogenesis in HT22 cells challenged by glutamate

Zimmerman,Mary A.; Hall,Mia; Qi,Qi; Mehta,Suresh L.; Chen,Guisheng; Li,P. Andy

doi:10.3892/etm.2021.10730

Ubisol Coenzyme Q10 promotes mitochondrial biogenesis in HT22 cells challenged by glutamate

Authors:
- Mary A. Zimmerman
- Mia Hall
- Qi Qi
- Suresh L. Mehta
- Guisheng Chen
- P. Andy Li
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Affiliations: Department of Pharmaceutical Sciences, Biomanufacturing Research Institute Biotechnology Enterprise (BRITE), North Carolina Central University, Durham, NC 27707, USA, Department of Neurology, General Hospital of Ningxia Medical University, Ningxia Key Laboratory of Cerebrocranial Diseases, Incubation Base of National Key Laboratory, Yinchuan, Ningxia 750004, P.R. China
Published online on: September 14, 2021 https://doi.org/10.3892/etm.2021.10730
Article Number: 1295
Copyright: © Zimmerman et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Glutamate‑induced excitotoxicity is a well-recognized cause of neuronal cell death. Nutritional supplementation with Coenzyme Q10 (CoQ10) has been previously demonstrated to serve neuro‑protective effects against glutamate‑induced excitotoxicity. The aim of the present study was to determine whether the protective effect of CoQ10 against glutamate toxicity could be attributed to stimulating mitochondrial biogenesis. Mouse hippocampal neuronal HT22 cells were incubated with glutamate with or without ubisol Q10. The results revealed that glutamate significantly decreased levels of mitochondrial biogenesis related proteins, including peroxisome proliferator‑activated receptor gamma coactivator (PGC)‑1α and nuclear respiratory factor (NRF)2. Additionally, glutamate reduced mitochondrial biogenesis, as determined using a mitochondrial biogenesis kit. Pretreatment with CoQ10 prevented decreases in phosphorylated (p)‑Akt, p‑cAMP response element‑binding protein, PGC‑1α, NRF2 and mitochondrial transcription factor A, increasing mitochondrial biogenesis. Taken together, the results described a novel mechanism of CoQ10‑induced neuroprotection and indicated a central role for mitochondrial biogenesis in protecting against glutamate‑induced excitotoxicity.

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