LGR6 promotes glioblastoma malignancy and chemoresistance by activating the Akt signaling pathway

  • Authors:
    • Yuan Yuan Cheng
    • Xue Yang
    • Xin Gao
    • Si Xin Song
    • Ming Feng Yang
    • Fang Min Xie
  • View Affiliations

  • Published online on: September 27, 2021     https://doi.org/10.3892/etm.2021.10798
  • Article Number: 1364
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Abstract

Chemoresistance is the primary cause of the poor outcome of glioblastoma multiforme (GBM) therapy. Leucine‑rich repeat‑containing G‑protein coupled receptor 6 (LGR6) is involved in the growth and proliferation of several types of cancer, including gastric cancer and ovarian cancer. Therefore, the aim of the present study was to investigate the role of LGR6 in GBM malignancy and chemoresistance. Cell counting kit‑8 and Matrigel®‑Transwell assays were conducted to assess GBM cell viability and invasion. The effect of LGR6 on cell cycle progression and activation of Akt signaling was analyzed by performing propidium iodide staining and western blotting, respectively. The results demonstrated that LGR6, a microRNA‑1236‑3p target candidate, promoted GBM cell viability and invasion, and mediated temozolomide sensitivity in SHG‑44 and U251 GBM cells. In addition, LGR6 triggered the activation of the Akt signaling pathway during GBM progression. Collectively, the results of the present study suggested that LGR6 promoted GBM malignancy and chemoresistance, at least in part, by activating the Akt signaling pathway. The results may aid with the identification of a novel therapeutic target and strategy for GBM.
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December-2021
Volume 22 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Cheng YY, Yang X, Gao X, Song SX, Yang MF and Xie FM: LGR6 promotes glioblastoma malignancy and chemoresistance by activating the Akt signaling pathway. Exp Ther Med 22: 1364, 2021
APA
Cheng, Y.Y., Yang, X., Gao, X., Song, S.X., Yang, M.F., & Xie, F.M. (2021). LGR6 promotes glioblastoma malignancy and chemoresistance by activating the Akt signaling pathway. Experimental and Therapeutic Medicine, 22, 1364. https://doi.org/10.3892/etm.2021.10798
MLA
Cheng, Y. Y., Yang, X., Gao, X., Song, S. X., Yang, M. F., Xie, F. M."LGR6 promotes glioblastoma malignancy and chemoresistance by activating the Akt signaling pathway". Experimental and Therapeutic Medicine 22.6 (2021): 1364.
Chicago
Cheng, Y. Y., Yang, X., Gao, X., Song, S. X., Yang, M. F., Xie, F. M."LGR6 promotes glioblastoma malignancy and chemoresistance by activating the Akt signaling pathway". Experimental and Therapeutic Medicine 22, no. 6 (2021): 1364. https://doi.org/10.3892/etm.2021.10798