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Long non‑coding RNA profiles in plasma exosomes of patients with gastric high‑grade intraepithelial neoplasia

  • Authors:
    • Feng Hu
    • Min Rao
    • Manli Zhang
    • Qingqing Meng
    • Minjie Wan
    • Xiuna Zhang
    • Lili Ding
    • Yanfang Jiang
  • View Affiliations / Copyright

    Affiliations: Department of Hepatology and Gastroenterology, The Second Part of First Hospital of Jilin University, Changchun, Jilin 130000, P.R. China, Central Laboratory, The First Hospital of Jilin University, Changchun, Jilin 130000, P.R. China, Intensive Care Unit, The First Hospital of Jilin University, Changchun, Jilin 130000, P.R. China, Key Laboratory of Organ Regeneration and Transplantation of The Ministry of Education, Genetic Diagnosis Center, The First Hospital of Jilin University, Changchun, Jilin 130000, P.R. China
    Copyright: © Hu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 1
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    Published online on: October 26, 2021
       https://doi.org/10.3892/etm.2021.10923
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Abstract

Long non‑coding (lnc) RNAs in circulating exosomes are a new class of promising cancer biomarkers; however, their expression in exosomes derived from gastric high‑grade intraepithelial neoplasia (GHGIN) has not been reported. In the present study, differentially expressed (DE) lncRNAs were analyzed in the peripheral blood collected from 5 patients with GHGIN and 5 healthy donors using high‑throughput sequencing. Reverse transcription‑quantitative PCR analysis was performed on 6 randomly selected DE lncRNAs to validate the reliability of the sequencing results. The potential roles of the DE lncRNAs in GHGIN were investigated using Gene Ontology (GO) and Kyoto Encyclopedia of Gene and Genome (KEGG) pathway enrichment analyses. A total of 25,145 lncRNAs were identified in all the samples and 83 DE lncRNAs were further screened, including 76 upregulated and 7 downregulated DE lncRNAs. GO and KEGG analyses predicted that the DE lncRNAs played notable roles in ‘protein/macromolecule glycosylation’, ‘regulation of protein ubiquitination’, ‘renin‑angiotensin system’ and ‘MAPK signaling pathways’. A lncRNA‑micro (mi)RNA‑mRNA interaction network was constructed and used to perform association analyses. It was found that 83 lncRNAs were abnormally expressed in GHGIN, with some potential functions associated with gastric cancer. Furthermore, the lncRNA‑miRNA‑mRNA interaction network indicated that 7 DE lncRNAs may play a notable role in the occurrence and development of GHGIN. The results of the present study showed the expression profiles of lncRNAs in human GHGIN, elucidated some of the molecular changes associated with GHGIN and improved the understanding of the molecular mechanisms underlying GHGIN and gastric cancer.
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Spandidos Publications style
Hu F, Rao M, Zhang M, Meng Q, Wan M, Zhang X, Ding L and Jiang Y: Long non‑coding RNA profiles in plasma exosomes of patients with gastric high‑grade intraepithelial neoplasia. Exp Ther Med 23: 1, 2022.
APA
Hu, F., Rao, M., Zhang, M., Meng, Q., Wan, M., Zhang, X. ... Jiang, Y. (2022). Long non‑coding RNA profiles in plasma exosomes of patients with gastric high‑grade intraepithelial neoplasia. Experimental and Therapeutic Medicine, 23, 1. https://doi.org/10.3892/etm.2021.10923
MLA
Hu, F., Rao, M., Zhang, M., Meng, Q., Wan, M., Zhang, X., Ding, L., Jiang, Y."Long non‑coding RNA profiles in plasma exosomes of patients with gastric high‑grade intraepithelial neoplasia". Experimental and Therapeutic Medicine 23.1 (2022): 1.
Chicago
Hu, F., Rao, M., Zhang, M., Meng, Q., Wan, M., Zhang, X., Ding, L., Jiang, Y."Long non‑coding RNA profiles in plasma exosomes of patients with gastric high‑grade intraepithelial neoplasia". Experimental and Therapeutic Medicine 23, no. 1 (2022): 1. https://doi.org/10.3892/etm.2021.10923
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Spandidos Publications style
Hu F, Rao M, Zhang M, Meng Q, Wan M, Zhang X, Ding L and Jiang Y: Long non‑coding RNA profiles in plasma exosomes of patients with gastric high‑grade intraepithelial neoplasia. Exp Ther Med 23: 1, 2022.
APA
Hu, F., Rao, M., Zhang, M., Meng, Q., Wan, M., Zhang, X. ... Jiang, Y. (2022). Long non‑coding RNA profiles in plasma exosomes of patients with gastric high‑grade intraepithelial neoplasia. Experimental and Therapeutic Medicine, 23, 1. https://doi.org/10.3892/etm.2021.10923
MLA
Hu, F., Rao, M., Zhang, M., Meng, Q., Wan, M., Zhang, X., Ding, L., Jiang, Y."Long non‑coding RNA profiles in plasma exosomes of patients with gastric high‑grade intraepithelial neoplasia". Experimental and Therapeutic Medicine 23.1 (2022): 1.
Chicago
Hu, F., Rao, M., Zhang, M., Meng, Q., Wan, M., Zhang, X., Ding, L., Jiang, Y."Long non‑coding RNA profiles in plasma exosomes of patients with gastric high‑grade intraepithelial neoplasia". Experimental and Therapeutic Medicine 23, no. 1 (2022): 1. https://doi.org/10.3892/etm.2021.10923
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