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Changes of farnesoid X receptor and Takeda G‑protein coupled receptor 5 following biliary tract external drainage in hemorrhagic shock

  • Authors:
    • Lu Wang
    • Huai-Wu He
    • Xiang Zhou
    • Yun Long
  • View Affiliations / Copyright

    Affiliations: Department of Critical Care Medicine, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100730, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 163
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    Published online on: December 21, 2021
       https://doi.org/10.3892/etm.2021.11086
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Abstract

Since biliary tract external drainage (BTED) is increasingly used to treat patients with shock, it is necessary to clarify pathophysiological changes following BTED in hemorrhagic shock (HS). The present study aimed to investigate the effect of BTED on farnesoid X receptor (FXR) and Takeda G‑protein coupled receptor 5 (TGR‑5) expression in HS. A total of 24 Sprague‑Dawley rats were randomly allocated to sham, BTED, HS and HS + BTED groups. Rat models of HS were induced by drawing blood from the femoral artery until a mean arterial pressure of 40±5 mmHg was achieved and maintained for 60 min. Rat models of BTED were induced by inserting a catheter into the bile duct. The distal end of the bile duct was ligated, and the catheter was passed through the rat flank to allow external collection of bile. Reverse transcription‑quantitative PCR, western blotting and immunohistochemistry were performed to detect changes in expression levels of FXR and TGR‑5 in the jejunum, ileum and liver. Expression levels of FXR and TGR‑5 increased significantly in jejunum and liver following HS (P<0.05). BTED significantly decreased expression levels of FXR in the liver (P<0.05) and TGR‑5 in the jejunum, ileum and liver (P<0.05). In conclusion, expression levels of FXR and TGR‑5 increased in HS but BTED decreased expression levels of FXR and TGR‑5 in HS.
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Copy and paste a formatted citation
Spandidos Publications style
Wang L, He H, Zhou X and Long Y: Changes of farnesoid X receptor and Takeda G‑protein coupled receptor 5 following biliary tract external drainage in hemorrhagic shock. Exp Ther Med 23: 163, 2022.
APA
Wang, L., He, H., Zhou, X., & Long, Y. (2022). Changes of farnesoid X receptor and Takeda G‑protein coupled receptor 5 following biliary tract external drainage in hemorrhagic shock. Experimental and Therapeutic Medicine, 23, 163. https://doi.org/10.3892/etm.2021.11086
MLA
Wang, L., He, H., Zhou, X., Long, Y."Changes of farnesoid X receptor and Takeda G‑protein coupled receptor 5 following biliary tract external drainage in hemorrhagic shock". Experimental and Therapeutic Medicine 23.2 (2022): 163.
Chicago
Wang, L., He, H., Zhou, X., Long, Y."Changes of farnesoid X receptor and Takeda G‑protein coupled receptor 5 following biliary tract external drainage in hemorrhagic shock". Experimental and Therapeutic Medicine 23, no. 2 (2022): 163. https://doi.org/10.3892/etm.2021.11086
Copy and paste a formatted citation
x
Spandidos Publications style
Wang L, He H, Zhou X and Long Y: Changes of farnesoid X receptor and Takeda G‑protein coupled receptor 5 following biliary tract external drainage in hemorrhagic shock. Exp Ther Med 23: 163, 2022.
APA
Wang, L., He, H., Zhou, X., & Long, Y. (2022). Changes of farnesoid X receptor and Takeda G‑protein coupled receptor 5 following biliary tract external drainage in hemorrhagic shock. Experimental and Therapeutic Medicine, 23, 163. https://doi.org/10.3892/etm.2021.11086
MLA
Wang, L., He, H., Zhou, X., Long, Y."Changes of farnesoid X receptor and Takeda G‑protein coupled receptor 5 following biliary tract external drainage in hemorrhagic shock". Experimental and Therapeutic Medicine 23.2 (2022): 163.
Chicago
Wang, L., He, H., Zhou, X., Long, Y."Changes of farnesoid X receptor and Takeda G‑protein coupled receptor 5 following biliary tract external drainage in hemorrhagic shock". Experimental and Therapeutic Medicine 23, no. 2 (2022): 163. https://doi.org/10.3892/etm.2021.11086
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