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pH regulates the lumen diameter of tissue‑engineered capillaries

  • Authors:
    • Xiaolin Wang
    • Jing Li
    • Yongqian Bian
    • Congying Zhao
    • Jinqing Li
    • Xueyong Li
  • View Affiliations / Copyright

    Affiliations: Department of Plastic and Burn Surgery, The Second Affiliated Hospital, Air Force Medical University, Xi'an, Shaanxi 710038, P.R. China, Department of Plastic and Burn Surgery, The Second Affiliated Hospital, Air Force Medical University, Xi'an, Shaanxi 710038, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 284
    |
    Published online on: February 15, 2022
       https://doi.org/10.3892/etm.2022.11212
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Abstract

Angiogenesis is vital in tissue engineering and the size of the capillary lumen diameter directly affects vascular function. Therefore, the involvement of the pH in the regulation of the capillary lumen diameter was investigated in the present study. The cytosolic pH of different pH medium groups was measured using flow cytometry. Bromodeoxyuridine staining and wound‑healing assays were performed to detect cell proliferation and migration, respectively. The expression of angiogenesis‑related genes was detected using reverse transcription‑quantitative PCR. In addition, cell tube formation under different pH conditions was assessed using a tube formation assay and a 3D Matrigel® model. The results indicated that a change in the pH value of the culture medium affected the cytosolic pH of the endothelial cells, which then led to a change in vascular diameter. When the medium's pH ranged from 7.4 to 7.6, the diameter of the lumen formed in the Matrigel was suitable for capillary formation in tissue engineering. The present results revealed an important role for the pH in the process of capillary formation and provided insight for pH regulation during endothelial cell tube formation and angiogenesis in tissue engineering.
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Copy and paste a formatted citation
Spandidos Publications style
Wang X, Li J, Bian Y, Zhao C, Li J and Li X: pH regulates the lumen diameter of tissue‑engineered capillaries. Exp Ther Med 23: 284, 2022.
APA
Wang, X., Li, J., Bian, Y., Zhao, C., Li, J., & Li, X. (2022). pH regulates the lumen diameter of tissue‑engineered capillaries. Experimental and Therapeutic Medicine, 23, 284. https://doi.org/10.3892/etm.2022.11212
MLA
Wang, X., Li, J., Bian, Y., Zhao, C., Li, J., Li, X."pH regulates the lumen diameter of tissue‑engineered capillaries". Experimental and Therapeutic Medicine 23.4 (2022): 284.
Chicago
Wang, X., Li, J., Bian, Y., Zhao, C., Li, J., Li, X."pH regulates the lumen diameter of tissue‑engineered capillaries". Experimental and Therapeutic Medicine 23, no. 4 (2022): 284. https://doi.org/10.3892/etm.2022.11212
Copy and paste a formatted citation
x
Spandidos Publications style
Wang X, Li J, Bian Y, Zhao C, Li J and Li X: pH regulates the lumen diameter of tissue‑engineered capillaries. Exp Ther Med 23: 284, 2022.
APA
Wang, X., Li, J., Bian, Y., Zhao, C., Li, J., & Li, X. (2022). pH regulates the lumen diameter of tissue‑engineered capillaries. Experimental and Therapeutic Medicine, 23, 284. https://doi.org/10.3892/etm.2022.11212
MLA
Wang, X., Li, J., Bian, Y., Zhao, C., Li, J., Li, X."pH regulates the lumen diameter of tissue‑engineered capillaries". Experimental and Therapeutic Medicine 23.4 (2022): 284.
Chicago
Wang, X., Li, J., Bian, Y., Zhao, C., Li, J., Li, X."pH regulates the lumen diameter of tissue‑engineered capillaries". Experimental and Therapeutic Medicine 23, no. 4 (2022): 284. https://doi.org/10.3892/etm.2022.11212
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