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Transcriptome analysis in an AEG‑1‑deficient neuronal HT22 cell line

  • Authors:
    • Kunmei Liu
    • Panpan Wan
    • Yue Huang
    • Bin Wang
    • Xuequan Wang
    • Rui Zhang
    • Le Guo
  • View Affiliations / Copyright

    Affiliations: Ningxia Key Laboratory of Cerebrocranial Disease, Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region 750004, P.R. China, Department of Microbiology and Biochemical Pharmacy, School of Pharmacy, Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region 750004, P.R. China, Ningxia Key Laboratory of Clinical and Pathogenic Microbiology, General Hospital of Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region 750004, P.R. China, Laboratory of Cellular and Molecular Radiation Oncology, The Affiliated Taizhou Hospital, Wenzhou Medical University, Taizhou, Zhejiang 317000, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 670
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    Published online on: September 13, 2022
       https://doi.org/10.3892/etm.2022.11607
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Abstract

Astrocyte elevated gene‑1 (AEG‑1) is a key regulatory factor of progression in multiple types of tumor and neurodegenerative disease development. AEG‑1 is associated with glutamate excitotoxicity due to its reported function of repressing excitatory amino acid transporter 2 expression in astrocytes. Although the function of AEG‑1 has been demonstrated in neurological disorders, such as Alzheimer's disease and amyotrophic lateral sclerosis, the underlying mechanism of neuronal AEG‑1 function remains unclear. The aim of the present study was to clarify the function and related mechanism of AEG‑1 in neurons. A stable AEG‑1‑deficient HT22 neuronal cell line was constructed using CRISPR/Cas9 gene‑editing technology. Reverse transcription‑quantitative PCR and western blotting were carried out to analyze the knockdown efficiency of AEG‑1‑deficient HT22 cell line. RNA Sanger sequencing analysis was performed in AEG‑1‑deficient HT22 cells and wild‑type HT22 cells without knockout (KO). Results from RNA sequencing revealed that AEG‑1 modulated neuronal morphology and development by regulating the expression of numerous genes, such as ubiquitin C, C‑X‑C motif chemokine ligand 1, MMP9, Notch1, neuropilin 1 and ephrin type‑A receptor 4. In addition, AEG‑1 deficiency impacted several signaling pathways by mediating cell survival differentiation, apoptosis, and migration; this included the TNF‑α pathway, the NF‑κB pathway, the MAPK signaling pathway, the Notch signaling pathway and Axon guidance. Downregulation in cellular ion homeostasis, including ion channel function and neurotransmitter release, were observed after knocking out AEG‑1 expression. Collectively, the present study provides insights into AEG‑1‑dependent gene regulation and signaling pathway transduction in neurons. The results of the present study may be applied for improving the understanding of AEG‑1‑associated central nervous system diseases.
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Liu K, Wan P, Huang Y, Wang B, Wang X, Zhang R and Guo L: Transcriptome analysis in an AEG‑1‑deficient neuronal HT22 cell line. Exp Ther Med 24: 670, 2022.
APA
Liu, K., Wan, P., Huang, Y., Wang, B., Wang, X., Zhang, R., & Guo, L. (2022). Transcriptome analysis in an AEG‑1‑deficient neuronal HT22 cell line. Experimental and Therapeutic Medicine, 24, 670. https://doi.org/10.3892/etm.2022.11607
MLA
Liu, K., Wan, P., Huang, Y., Wang, B., Wang, X., Zhang, R., Guo, L."Transcriptome analysis in an AEG‑1‑deficient neuronal HT22 cell line". Experimental and Therapeutic Medicine 24.5 (2022): 670.
Chicago
Liu, K., Wan, P., Huang, Y., Wang, B., Wang, X., Zhang, R., Guo, L."Transcriptome analysis in an AEG‑1‑deficient neuronal HT22 cell line". Experimental and Therapeutic Medicine 24, no. 5 (2022): 670. https://doi.org/10.3892/etm.2022.11607
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Spandidos Publications style
Liu K, Wan P, Huang Y, Wang B, Wang X, Zhang R and Guo L: Transcriptome analysis in an AEG‑1‑deficient neuronal HT22 cell line. Exp Ther Med 24: 670, 2022.
APA
Liu, K., Wan, P., Huang, Y., Wang, B., Wang, X., Zhang, R., & Guo, L. (2022). Transcriptome analysis in an AEG‑1‑deficient neuronal HT22 cell line. Experimental and Therapeutic Medicine, 24, 670. https://doi.org/10.3892/etm.2022.11607
MLA
Liu, K., Wan, P., Huang, Y., Wang, B., Wang, X., Zhang, R., Guo, L."Transcriptome analysis in an AEG‑1‑deficient neuronal HT22 cell line". Experimental and Therapeutic Medicine 24.5 (2022): 670.
Chicago
Liu, K., Wan, P., Huang, Y., Wang, B., Wang, X., Zhang, R., Guo, L."Transcriptome analysis in an AEG‑1‑deficient neuronal HT22 cell line". Experimental and Therapeutic Medicine 24, no. 5 (2022): 670. https://doi.org/10.3892/etm.2022.11607
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