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lncRNA XIST/miR‑129‑2‑3p axis targets CCP110 to regulate the proliferation, invasion and migration of endometrial cancer cells

  • Authors:
    • Shu Chen
    • Yaozhong Liang
    • Yuan Shen
    • Xiaoyu Wang
  • View Affiliations / Copyright

    Affiliations: Department of Gynecology and Obstetrics, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510630, P.R. China, Department of Orthopedics, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510630, P.R. China, Department of Gynecology and Obstetrics, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510630, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 159
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    Published online on: February 22, 2023
       https://doi.org/10.3892/etm.2023.11858
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Abstract

Centromere coiled‑coil protein 110 (CCP110) plays a role in the development of several types of cancer; however, its regulatory mechanism and role in endometrial cancer is unclear. The present study revealed that CCP110 is regulated by a signaling pathway involving microRNA (miR/miRNA)‑129‑2‑3p and the long non‑coding RNA (lncRNA) X‑inactive‑specific transcript (XIST), and plays a role in controlling the proliferation, migration and invasion of endometrial cancer cells. CCP110 was upregulated in human endometrial cancer tissues, as revealed by immunohistochemistry, and high expression of the protein was related to reduced overall survival of the patients. Genetic knockdown of CCP110 by small interfering RNA promoted apoptosis and suppressed the proliferation, migration, invasion and colony formation of endometrial cancer cells significantly in the endometrial cancer Ishikawa and HEC‑1B cell lines, as assessed by flow cytometry, and Cell Counting Kit‑8, Transwell and colony formation assays. A bioinformatics analysis and luciferase reporter assay revealed that CCP110 is a target of miR‑129‑2‑3p. Overexpression of miR‑129‑2‑3p mimic fragments inhibited the proliferation, migration and invasion of endometrial cancer cells significantly, while co‑overexpression of CCP110 counteracted these inhibitory effects. The expression level of the lncRNA XIST was upregulated significantly in endometrial cancer tissues, as assessed by reverse transcription‑quantitative PCR assay, while that of miR‑129‑2‑3p was downregulated significantly. A bioinformatics analysis and luciferase reporter assay showed that XIST could inhibit miR‑129‑2‑3p via a miRNA sponge effect. Furthermore, co‑overexpression of XIST antagonized the inhibitory effect of the miR‑129‑2‑3p mimic on the luciferase reporter gene signal and protein expression of CCP110. Co‑overexpression of XIST also abolished the inhibitory effect of the miR‑129‑2‑3p mimic on the proliferation, migration and invasion of endometrial cancer cells. Overall, these data identified a novel regulatory mechanism of CCP110 involving XIST and miR‑129‑2‑3p, which affected the development of endometrial carcinoma. CCP110, XIST and miR‑129‑2‑3p could represent novel targets for the clinical treatment of endometrial cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Chen S, Liang Y, Shen Y and Wang X: lncRNA XIST/miR‑129‑2‑3p axis targets CCP110 to regulate the proliferation, invasion and migration of endometrial cancer cells. Exp Ther Med 25: 159, 2023.
APA
Chen, S., Liang, Y., Shen, Y., & Wang, X. (2023). lncRNA XIST/miR‑129‑2‑3p axis targets CCP110 to regulate the proliferation, invasion and migration of endometrial cancer cells. Experimental and Therapeutic Medicine, 25, 159. https://doi.org/10.3892/etm.2023.11858
MLA
Chen, S., Liang, Y., Shen, Y., Wang, X."lncRNA XIST/miR‑129‑2‑3p axis targets CCP110 to regulate the proliferation, invasion and migration of endometrial cancer cells". Experimental and Therapeutic Medicine 25.4 (2023): 159.
Chicago
Chen, S., Liang, Y., Shen, Y., Wang, X."lncRNA XIST/miR‑129‑2‑3p axis targets CCP110 to regulate the proliferation, invasion and migration of endometrial cancer cells". Experimental and Therapeutic Medicine 25, no. 4 (2023): 159. https://doi.org/10.3892/etm.2023.11858
Copy and paste a formatted citation
x
Spandidos Publications style
Chen S, Liang Y, Shen Y and Wang X: lncRNA XIST/miR‑129‑2‑3p axis targets CCP110 to regulate the proliferation, invasion and migration of endometrial cancer cells. Exp Ther Med 25: 159, 2023.
APA
Chen, S., Liang, Y., Shen, Y., & Wang, X. (2023). lncRNA XIST/miR‑129‑2‑3p axis targets CCP110 to regulate the proliferation, invasion and migration of endometrial cancer cells. Experimental and Therapeutic Medicine, 25, 159. https://doi.org/10.3892/etm.2023.11858
MLA
Chen, S., Liang, Y., Shen, Y., Wang, X."lncRNA XIST/miR‑129‑2‑3p axis targets CCP110 to regulate the proliferation, invasion and migration of endometrial cancer cells". Experimental and Therapeutic Medicine 25.4 (2023): 159.
Chicago
Chen, S., Liang, Y., Shen, Y., Wang, X."lncRNA XIST/miR‑129‑2‑3p axis targets CCP110 to regulate the proliferation, invasion and migration of endometrial cancer cells". Experimental and Therapeutic Medicine 25, no. 4 (2023): 159. https://doi.org/10.3892/etm.2023.11858
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