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Profile analysis of differentially expressed long non‑coding RNAs in metabolic memory induced by high glucose in human umbilical vein endothelial cells

  • Authors:
    • Jingya Cheng
    • Anqi Huang
    • Ji Cheng
    • Xiaoyan Pei
    • Lei Yu
    • Guoxi Jin
    • Erqin Xu
  • View Affiliations / Copyright

    Affiliations: Department of Endocrinology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, P.R. China, Department of Physical Diagnostics, Bengbu Medical College, Bengbu, Anhui 233030, P.R. China
    Copyright: © Cheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 288
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    Published online on: May 2, 2023
       https://doi.org/10.3892/etm.2023.11987
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Abstract

Numerous long non‑coding RNAs (lncRNAs) are dysregulated in the hyperglycemia‑induced phenomenon of metabolic memory (MM). In the present study, the significance of these lncRNAs in MM was explored by screening for MM‑involved differentially expressed lncRNAs (MMDELs) in human umbilical vein endothelial cells (HUVECs) induced by high glucose. A total of nine HUVEC samples were divided into three groups to mimic conditions of low and high glucose environments, as well as induce the state of metabolic memory. The expression of lncRNAs was profiled using RNA sequencing. Bioinformatic analysis was performed using the Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes databases to explore the parental genes from which the lncRNAs are transcribed and target genes of the MMDELs and generate enrichment datasets. Reverse transcription‑quantitative PCR was performed to validate the expression levels of the selected lncRNAs. The present study identified 308 upregulated and 157 downregulated MMDELs, which were enriched in numerous physiologic processes. Key functional enrichment terms included ‘cell cycle’, ‘oocyte meiosis’ and ‘p53 signaling pathway’. In conclusion, certain MMDELs may regulate the expression level of highly associated mRNAs through various mechanisms and pathways, thereby interfering with several processes, such as the regulation of the cell cycle, and affecting vascular endothelial cell function. Furthermore, the disorders of these lncRNAs can be retained in MM, further investigation into the functions of these lncRNAs may result in novel insights and treatments, which could help control MM in patients with diabetes.
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Copy and paste a formatted citation
Spandidos Publications style
Cheng J, Huang A, Cheng J, Pei X, Yu L, Jin G and Xu E: Profile analysis of differentially expressed long non‑coding RNAs in metabolic memory induced by high glucose in human umbilical vein endothelial cells. Exp Ther Med 25: 288, 2023.
APA
Cheng, J., Huang, A., Cheng, J., Pei, X., Yu, L., Jin, G., & Xu, E. (2023). Profile analysis of differentially expressed long non‑coding RNAs in metabolic memory induced by high glucose in human umbilical vein endothelial cells. Experimental and Therapeutic Medicine, 25, 288. https://doi.org/10.3892/etm.2023.11987
MLA
Cheng, J., Huang, A., Cheng, J., Pei, X., Yu, L., Jin, G., Xu, E."Profile analysis of differentially expressed long non‑coding RNAs in metabolic memory induced by high glucose in human umbilical vein endothelial cells". Experimental and Therapeutic Medicine 25.6 (2023): 288.
Chicago
Cheng, J., Huang, A., Cheng, J., Pei, X., Yu, L., Jin, G., Xu, E."Profile analysis of differentially expressed long non‑coding RNAs in metabolic memory induced by high glucose in human umbilical vein endothelial cells". Experimental and Therapeutic Medicine 25, no. 6 (2023): 288. https://doi.org/10.3892/etm.2023.11987
Copy and paste a formatted citation
x
Spandidos Publications style
Cheng J, Huang A, Cheng J, Pei X, Yu L, Jin G and Xu E: Profile analysis of differentially expressed long non‑coding RNAs in metabolic memory induced by high glucose in human umbilical vein endothelial cells. Exp Ther Med 25: 288, 2023.
APA
Cheng, J., Huang, A., Cheng, J., Pei, X., Yu, L., Jin, G., & Xu, E. (2023). Profile analysis of differentially expressed long non‑coding RNAs in metabolic memory induced by high glucose in human umbilical vein endothelial cells. Experimental and Therapeutic Medicine, 25, 288. https://doi.org/10.3892/etm.2023.11987
MLA
Cheng, J., Huang, A., Cheng, J., Pei, X., Yu, L., Jin, G., Xu, E."Profile analysis of differentially expressed long non‑coding RNAs in metabolic memory induced by high glucose in human umbilical vein endothelial cells". Experimental and Therapeutic Medicine 25.6 (2023): 288.
Chicago
Cheng, J., Huang, A., Cheng, J., Pei, X., Yu, L., Jin, G., Xu, E."Profile analysis of differentially expressed long non‑coding RNAs in metabolic memory induced by high glucose in human umbilical vein endothelial cells". Experimental and Therapeutic Medicine 25, no. 6 (2023): 288. https://doi.org/10.3892/etm.2023.11987
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