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International Journal of Molecular Medicine
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2014-January Volume 33 Issue 1

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Article

Effects of mulberry ethanol extracts on hydrogen peroxide-induced oxidative stress in pancreatic β-cells

  • Authors:
    • Young Rae Kim
    • Jong Seok Lee
    • Ki Rim Lee
    • Young Eon Kim
    • Nam In Baek
    • Eock Kee Hong
  • View Affiliations / Copyright

    Affiliations: Department of Bioengineering and Technology, Kangwon National University, Chuncheon, Kangwon‑do 200‑701, Republic of Korea, Korea Food Research Institute, Songnam-si, Kyunggi-do 463-746, Republic of Korea, Department of Oriental Medicinal Materials and Processing, Kyung Hee University, Yongin-si, Gyeonggi-do 446-701, Republic of Korea
  • Pages: 128-134
    |
    Published online on: October 23, 2013
       https://doi.org/10.3892/ijmm.2013.1534
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Abstract

Reactive oxygen species (ROS) are key mediators of mammalian cellular damage and are associated with diseases such as aging, arteriosclerosis, inflammation, rheumatoid arthritis and diabetes. Type 1 diabetes develops upon the destruction of pancreatic β-cells, which is partly due to ROS activity. In this study, we investigated the cytoprotective and anti-oxidative effects of fractionated mulberry extracts in mouse insulin-producing pancreatic β-cells (MIN6N cells). Treatment with hydrogen peroxide (H2O2) induced significant cell death and increased intracellular ROS levels, lipid peroxidation and DNA fragmentation in the MIN6N cells. Fractionated mulberry extracts significantly reduced the H2O2-dependent production of intracellular ROS, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals and lipid peroxidation. In addition, mulberry extracts inhibited DNA fragmentation induced by H2O2. Thus, the antioxidant properties of mulberry extracts in pancreatic β-cells may be exploited for the prevention or treatment of type 1 diabetes.
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1 

Blois MS: Free Radicals in Biological Systems. Science. 132:306–307. 1960. View Article : Google Scholar : PubMed/NCBI

2 

Diplock AT, Charleux JL, Crozier-Willi G, et al: Functional food science and defence against reactive oxidative species. Br J Nutr. 80(Suppl 1): S77–S112. 1998. View Article : Google Scholar : PubMed/NCBI

3 

Dalton TP, Shertzer HG and Puga A: Regulation of gene expression by reactive oxygen. Annu Rev Pharmacol Toxicol. 39:67–101. 1999. View Article : Google Scholar : PubMed/NCBI

4 

Blumberg J: Use of biomarkers of oxidative stress in research studies. J Nutr. 134:3188S–3189S. 2004.PubMed/NCBI

5 

Lizard G, Fournel S, Genestier L, et al: Kinetics of plasma membrane and mitochondrial alterations in cells undergoing apoptosis. Cytometry. 21:275–283. 1995. View Article : Google Scholar : PubMed/NCBI

6 

Lee JH, Lee JS, Kim YR, et al: Hispidin isolated from Phellinus linteus protects against hydrogen peroxide-induced oxidative stress in pancreatic MIN6N β-cells. J Med Food. 14:1431–1438. 2011.

7 

Maritim AC, Sanders RA and Watkins JB III: Diabetes, oxidative stress, and antioxidants: a review. J Biochem Mol Toxicol. 17:24–38. 2003. View Article : Google Scholar

8 

Zhong J, Rao X, Xu JF, Yang P and Wang CY: The role of endoplasmic reticulum stress in autoimmune-mediated beta-cell destruction in type 1 diabetes. Exp Diabetes Res. 2012:2389802012. View Article : Google Scholar : PubMed/NCBI

9 

Bach JF: Insulin-dependent diabetes mellitus as an autoimmune disease. Endocr Rev. 15:516–542. 1994. View Article : Google Scholar : PubMed/NCBI

10 

Matsuoka T, Kajimoto Y, Watada H, et al: Glycation-dependent, reactive oxygen species-mediated suppression of the insulin gene promoter activity in HIT cells. J Clin Invest. 99:144–150. 1997. View Article : Google Scholar : PubMed/NCBI

11 

Allen DA, Yaqoob MM and Harwood SM: Mechanisms of high glucose-induced apoptosis and its relationship to diabetic complications. J Nutr Biochem. 16:705–713. 2005. View Article : Google Scholar : PubMed/NCBI

12 

Davis KL, Tangirala M, Meyers JL and Wei W: Real-world comparative outcomes of US type 2 diabetes patients initiating analog basal insulin therapy. Curr Med Res Opin. 9:1083–1091. 2013. View Article : Google Scholar : PubMed/NCBI

13 

Rolo AP and Palmeira CM: Diabetes and mitochondrial function: role of hyperglycemia and oxidative stress. Toxicol Appl Pharmacol. 212:167–178. 2006. View Article : Google Scholar : PubMed/NCBI

14 

Giugliano D, Ceriello A and Paolisso G: Oxidative stress and diabetic vascular complications. Diabetes Care. 19:257–267. 1996. View Article : Google Scholar : PubMed/NCBI

15 

Samyshkin Y, Guillermin AL, Best JH, Brunell SC and Lloyd A: Long-term cost-utility analysis of exenatide once weekly versus insulin glargine for the treatment of type 2 diabetes patients in the US. J Med Econ. 15(Suppl 2): S6–S13. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Koster I, Schubert I and Huppertz E: Follow up of the CoDiM-Study: Cost of diabetes mellitus 2000–2009. Dtsch Med Wochenschr. 137:1013–1016. 2012.(In German).

17 

Riblett B: Diabetes mellitus: the undiscussed side effect: sexual dysfunction. RN (For Managers). 46:40–41. 1983.PubMed/NCBI

18 

Pan G and Lou C: Isolation of an 1-aminocyclopropane-1-carboxylate oxidase gene from mulberry (Morus alba L.) and analysis of the function of this gene in plant development and stresses response. J Plant Physiol. 165:1204–1213. 2008. View Article : Google Scholar : PubMed/NCBI

19 

Kim GN, Kwon YI and Jang HD: Mulberry leaf extract reduces postprandial hyperglycemia with few side effects by inhibiting a-glucosidase in normal rats. J Med Food. 14:712–717. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Kwon HJ, Chung JY, Kim JY and Kwon O: Comparison of 1-deoxynojirimycin and aqueous mulberry leaf extract with emphasis on postprandial hypoglycemic effects: in vivo and in vitro studies. J Agric Food Chem. 59:3014–3019. 2011. View Article : Google Scholar : PubMed/NCBI

21 

Zheng ZP, Cheng KW, Zhu Q, Wang XC, Lin ZX and Wang M: Tyrosinase inhibitory constituents from the roots of Morus nigra: a structure-activity relationship study. J Agric Food Chem. 58:5368–5373. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Jin YS, Lee MJ, Han W, Heo SI, Sohn SI and Wang MH: Antioxidant effects and hepatoprotective activity of 2,5-dihydroxy-4,3′-di(beta-d-glucopyranosyloxy)-trans-stilbene from Morus bombycis Koidzumi roots on CCl4-induced liver damage. Free Radic Res. 40:986–992. 2006.PubMed/NCBI

23 

Jang JS, Lee JS, Lee JH, et al: Hispidin produced from Phellinus linteus protects pancreatic beta-cells from damage by hydrogen peroxide. Arch Pharm Res. 33:853–861. 2010.

24 

Ohkawa H, Ohishi N and Yagi K: Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 95:351–358. 1979. View Article : Google Scholar : PubMed/NCBI

25 

Kang KA, Kim JS, Zhang R, et al: KIOM-4 protects against oxidative stress-induced mitochondrial damage in pancreatic β-cells via its antioxidant effects. Evid Based Complement Alternat Med. 2011:9786822011.PubMed/NCBI

26 

Gschwind M and Huber G: Apoptotic cell death induced by beta-amyloid 1–42 peptide is cell type dependent. J Neurochem. 65:292–300. 1995.

27 

Ortega-Camarillo C, Guzman-Grenfell AM, Garcia-Macedo R, et al: Hyperglycemia induces apoptosis and p53 mobilization to mitochondria in RINm5F cells. Mol Cell Biochem. 281:163–171. 2006. View Article : Google Scholar

28 

Evans JL, Goldfine ID, Maddux BA and Grodsky GM: Are oxidative stress-activated signaling pathways mediators of insulin resistance and beta-cell dysfunction? Diabetes. 52:1–8. 2003. View Article : Google Scholar : PubMed/NCBI

29 

Zhang R, Kim JS, Kang KA, Piao MJ, Kim KC and Hyun JW: Protective mechanism of KIOM-4 in streptozotocin-induced pancreatic β-cells damage is involved in the inhibition of endoplasmic reticulum stress. Evid Based Complement Alternat Med. 2011:2319382011.PubMed/NCBI

30 

Patel KD, Scarano FJ, Kondo M, Hurta RA and Neto CC: Proanthocyanidin-rich extracts from cranberry fruit (Vaccinium macrocarpon Ait.) selectively inhibit the growth of human pathogenic fungi Candida spp and Cryptococcus neoformans. J Agric Food Chem. 59:12864–12873. 2011.PubMed/NCBI

31 

Kim TG, Kang SY, Jung KK, et al: Antiviral activities of extracts isolated from Terminalis chebula Retz., Sanguisorba officinalis L, Rubus coreanus Miq and Rheum palmatum L against hepatitis B virus. Phytother Res. 15:718–720. 2001.PubMed/NCBI

32 

Ku CS and Mun SP: Antioxidant activities of ethanol extracts from seeds in fresh Bokbunja (Rubus coreanus Miq.) and wine processing waste. Bioresour Technol. 99:4503–4509. 2008. View Article : Google Scholar : PubMed/NCBI

33 

Yang HM, Oh SM, Lim SS, Shin HK, Oh YS and Kim JK: Antiinflammatory activities of Rubus coreanus depend on the degree of fruit ripening. Phytother Res. 22:102–107. 2008.

34 

Im SE, Nam TG, Lee H, et al: Anthocyanins in the ripe fruits of Rubus coreanus Miquel and their protective effect on neuronal PC-12 cells. Food Chem. 139:604–610. 2013.PubMed/NCBI

35 

Liu JD: Study of the etiology of low-fever among female silk-weaving workers: extrinsic allergic alveolitis due to mulberry silk dust exposure. Zhonghua Yu Fang Yi Xue Za Zhi. 19:354–357. 1985.(In Chinese).

36 

Yang TP, Lee HJ, Ou TT, Chang YJ and Wang CJ: Mulberry leaf polyphenol extract induced apoptosis involving regulation of adenosine monophosphate-activated protein kinase/fatty acid synthase in a p53-negative hepatocellular carcinoma cell. J Agric Food Chem. Jun 26–2012.(Epub ahead of print).

37 

Kaewkaen P, Tong-Un T, Wattanathorn J, Muchimapura S, Kaewrueng W and Wongcharoenwanakit S: Mulberry fruit extract protects against memory impairment and hippocampal damage in animal model of vascular dementia. Evid Based Complement Alternat Med. 2012:2635202012.PubMed/NCBI

38 

Kaneto H, Fujii J, Myint T, et al: Reducing sugars trigger oxidative modification and apoptosis in pancreatic beta-cells by provoking oxidative stress through the glycation reaction. Biochem J. 320:855–863. 1996.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Kim YR, Lee JS, Lee KR, Kim YE, Baek NI and Hong EK: Effects of mulberry ethanol extracts on hydrogen peroxide-induced oxidative stress in pancreatic β-cells. Int J Mol Med 33: 128-134, 2014.
APA
Kim, Y.R., Lee, J.S., Lee, K.R., Kim, Y.E., Baek, N.I., & Hong, E.K. (2014). Effects of mulberry ethanol extracts on hydrogen peroxide-induced oxidative stress in pancreatic β-cells. International Journal of Molecular Medicine, 33, 128-134. https://doi.org/10.3892/ijmm.2013.1534
MLA
Kim, Y. R., Lee, J. S., Lee, K. R., Kim, Y. E., Baek, N. I., Hong, E. K."Effects of mulberry ethanol extracts on hydrogen peroxide-induced oxidative stress in pancreatic β-cells". International Journal of Molecular Medicine 33.1 (2014): 128-134.
Chicago
Kim, Y. R., Lee, J. S., Lee, K. R., Kim, Y. E., Baek, N. I., Hong, E. K."Effects of mulberry ethanol extracts on hydrogen peroxide-induced oxidative stress in pancreatic β-cells". International Journal of Molecular Medicine 33, no. 1 (2014): 128-134. https://doi.org/10.3892/ijmm.2013.1534
Copy and paste a formatted citation
x
Spandidos Publications style
Kim YR, Lee JS, Lee KR, Kim YE, Baek NI and Hong EK: Effects of mulberry ethanol extracts on hydrogen peroxide-induced oxidative stress in pancreatic β-cells. Int J Mol Med 33: 128-134, 2014.
APA
Kim, Y.R., Lee, J.S., Lee, K.R., Kim, Y.E., Baek, N.I., & Hong, E.K. (2014). Effects of mulberry ethanol extracts on hydrogen peroxide-induced oxidative stress in pancreatic β-cells. International Journal of Molecular Medicine, 33, 128-134. https://doi.org/10.3892/ijmm.2013.1534
MLA
Kim, Y. R., Lee, J. S., Lee, K. R., Kim, Y. E., Baek, N. I., Hong, E. K."Effects of mulberry ethanol extracts on hydrogen peroxide-induced oxidative stress in pancreatic β-cells". International Journal of Molecular Medicine 33.1 (2014): 128-134.
Chicago
Kim, Y. R., Lee, J. S., Lee, K. R., Kim, Y. E., Baek, N. I., Hong, E. K."Effects of mulberry ethanol extracts on hydrogen peroxide-induced oxidative stress in pancreatic β-cells". International Journal of Molecular Medicine 33, no. 1 (2014): 128-134. https://doi.org/10.3892/ijmm.2013.1534
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