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Article Open Access

Rutin promotes the formation and osteogenic differentiation of human periodontal ligament stem cell sheets in vitro

  • Authors:
    • Bin Zhao
    • Yunpeng Zhang
    • Yixuan Xiong
    • Xin Xu
  • View Affiliations / Copyright

    Affiliations: School of Stomatology, Shandong University, Jinan, Shandong 250012, P.R. China
    Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2289-2297
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    Published online on: October 25, 2019
       https://doi.org/10.3892/ijmm.2019.4384
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Abstract

Cell sheet technology is a novel tissue engineering technology that has been rapidly developed in recent years. As a novel technology, cell sheet technology is expected to become one of the preferred methods for cell transplantation. The present study investigated the biological effects of rutin on the formation of periodontal ligament stem cell (PDLSC) sheets and their resultant osteogenic properties. The results of Cell Counting Kit‑8 (CCK‑8) assay demonstrated that a concentration of 1x10‑6 mol/l rutin promoted the proliferation of PDLSCs more effectively compared with other designed concentrations. Rutin‑modified cell sheets could be induced by complete medium supplemented with 20 µg/ml vitamin C (VC) and 1x10‑6 mol/l rutin. Rutin‑modified cell sheets appeared thicker and more compact compared with the VC‑induced PDLSC sheets, demonstrating more layers of cells (3 or 4 layers), which secreted a richer extracellular matrix (ECM). Furthermore, the improved cell sheets exhibited varying degrees of increases in the mRNA and protein expression of collagen type I (COL1), alkaline phosphatase (ALP), runt‑related transcription factor 2 (RUNX2) and osteopontin (OPN). Combined treatment with VC and rutin promoted the formation of PDLSC sheets and enhanced the osteogenic differentiation potential of the cell sheets. Therefore, rutin‑modified cell sheets of PDLSCs are expected to play an important role in the treatment of periodontal tissue regeneration by stem cells.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao B, Zhang Y, Xiong Y and Xu X: Rutin promotes the formation and osteogenic differentiation of human periodontal ligament stem cell sheets in vitro. Int J Mol Med 44: 2289-2297, 2019.
APA
Zhao, B., Zhang, Y., Xiong, Y., & Xu, X. (2019). Rutin promotes the formation and osteogenic differentiation of human periodontal ligament stem cell sheets in vitro. International Journal of Molecular Medicine, 44, 2289-2297. https://doi.org/10.3892/ijmm.2019.4384
MLA
Zhao, B., Zhang, Y., Xiong, Y., Xu, X."Rutin promotes the formation and osteogenic differentiation of human periodontal ligament stem cell sheets in vitro". International Journal of Molecular Medicine 44.6 (2019): 2289-2297.
Chicago
Zhao, B., Zhang, Y., Xiong, Y., Xu, X."Rutin promotes the formation and osteogenic differentiation of human periodontal ligament stem cell sheets in vitro". International Journal of Molecular Medicine 44, no. 6 (2019): 2289-2297. https://doi.org/10.3892/ijmm.2019.4384
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao B, Zhang Y, Xiong Y and Xu X: Rutin promotes the formation and osteogenic differentiation of human periodontal ligament stem cell sheets in vitro. Int J Mol Med 44: 2289-2297, 2019.
APA
Zhao, B., Zhang, Y., Xiong, Y., & Xu, X. (2019). Rutin promotes the formation and osteogenic differentiation of human periodontal ligament stem cell sheets in vitro. International Journal of Molecular Medicine, 44, 2289-2297. https://doi.org/10.3892/ijmm.2019.4384
MLA
Zhao, B., Zhang, Y., Xiong, Y., Xu, X."Rutin promotes the formation and osteogenic differentiation of human periodontal ligament stem cell sheets in vitro". International Journal of Molecular Medicine 44.6 (2019): 2289-2297.
Chicago
Zhao, B., Zhang, Y., Xiong, Y., Xu, X."Rutin promotes the formation and osteogenic differentiation of human periodontal ligament stem cell sheets in vitro". International Journal of Molecular Medicine 44, no. 6 (2019): 2289-2297. https://doi.org/10.3892/ijmm.2019.4384
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