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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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In vitro and in vivo inhibition of human Fanconi anemia-associated head and neck squamous cell carcinoma by a novel nutrient mixture

  • Authors:
    • M. W. Roomi
    • T. Kalinovsky
    • N. W. Roomi
    • A. Niedzwiecki
    • M. Rath
  • View Affiliations / Copyright

    Affiliations: Dr Rath Research Institute, Santa Clara, CA 95050, USA
    Copyright: © Roomi et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 1996-2004
    |
    Published online on: September 24, 2012
       https://doi.org/10.3892/ijo.2012.1639
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Abstract

Head and neck squamous cell carcinoma (HNSCC) and acute myeloid leukemia are the major causes of mortality and morbidity in Fanconi anemia (FA) patients. The objective of this study was to investigate the antineoplastic activity of a novel antineoplastic nutrient mixture (NM) (containing lysine, proline, ascorbic acid and green tea extract) in human FA-associated HNSCC (FA HNSCC) in vitro and in vivo. The human FA HNSCC cell line, OHSU-974 (Fanconi Anemia Research Fund), was cultured in RPMI medium supplemented with 20% FBS and antibiotics. At near confluence, cells were treated in triplicate with various concentrations of NM: 0, 50, 100, 250, 500 and 1,000 µg/ml. Cells were also treated with phorbol 12-myristate 13-acetate (PMA) to induce matrix metalloproteinase (MMP)-9 activity. Cell proliferation was detected by MTT assay, the secretion of MMPs by gelatinase zymo­graphy, cell invasion through Matrigel, cell migration by a scratch test and morphology by hematoxylin and eosin (H&E) staining. In vivo, athymic male nude mice (n=12) were inoculated with 3x106 OHSU‑974 cells subcutaneously and randomly divided into 2 groups: group A was fed a regular diet and group B a regular diet supplemented with 1% NM. Four weeks later, the mice were sacrificed and their tumors were excised, weighed and processed for histological analysis. NM inhibited the growth of OHSU-974 tumors by 47% and tumor burden by 50%. At lower concentrations, NM demonstrated no effect on proliferation, but at 1,000 µg/ml a 40% toxicity was observed. Zymography revealed the MMP-2 and PMA-induced MMP-9 secretion. NM suppressed the secretion of both MMPs in a dose-dependent manner, with a virtual inhibition at 500 µg/ml. NM inhibited OHSU-974 cell invasion through Matrigel in a dose-dependent manner with a complete block at 1,000 µg/ml. H&E staining showed no morphological changes below 500 µg/ml. These results suggest that NM has potential therapeutic use in the treatment of human FA HNSCC.
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1. 

BP AlterMH GreeneI VelasquezPS RosenbergCancer in Fanconi anemiaBlood10120722073200310.1182/blood-2002-11-359712584146

2. 

DI KutlerAD AuerbachJH SatagopanHigh incidence of head and neck squamous cell carcinoma in patients with Fanconi anemiaArch Otolaryngol Head Neck Surg192106112200310.1001/archotol.129.1.106

3. 

B SinghHead and neck squamous carcinoma in Fanconi anemia patientsFanconi Anemia: Guidelines for Diagnosis and ManagementME EilerD FohnmayerL ForhnmayerK LarsenJ Owen3rd editionFanconi Anemia Research Fund, IncEugene, OR2502632008

4. 

MW RoomiN RoomiV IvanovT KalinovskyA NiedzwieckiM RathInhibitory effect of a mixture containing ascorbic acid, lysine, proline and green tea extract on critical parameters in angiogenesisOncol Rep14807815200516142336

5. 

MW RoomiV IvanovT KalinovskyA NiedzwieckiM RathInhibition of pulmonary metastasis of melanoma B16FO cells in C57BL/6 mice by a nutrient mixture consisting of ascorbic acid, lysine, proline, arginine, and green tea extractExp Lung Res32517530200610.1080/0190214060109855217169857

6. 

A NiedzwieckiMW RoomiT KalinovskyM RathMicronutrient synergy - a new tool in effective control of metastasis and other key mechanisms of cancerCancer Metastasis Rev29529543201010.1007/s10555-010-9244-120717705

7. 

MJ DuffyThe role of proteolytic enzymes in cancer invasion and metastasisClin Exp Metastasis10145155199210.1007/BF001327461582084

8. 

H KawamataD UchidaH HamanoT Kimura-YanagawaKI NakashiroS HinoF OmoteharaH YoshidaM SatoActive MMP-2 in cancer cell nests of oral cancer patients: Correlation with lymph node metastasisInt J Oncol1369970419989735398

9. 

BP PatelSV ShahSN ShuklaPM ShabPS PatelClinical significance of MMP-2 and MMP-9 in patients with oral cancerHead Neck29564572200710.1002/hed.2056117252594

10. 

A KatayamaN BandohK KishibeM TakaharaT OginoS NonakaY HarabuchiExpression of matrix metalloproteinases in early-stage oral squamous cell carcinoma as predictive indicators of tumor metastases and prognosisClin Cancer Res10634640200410.1158/1078-0432.CCR-0864-0214760086

11. 

F ReidelK GötteJ SchwalbW BerglerK HörmannExpression of 92-kDa type IV collagenase correlates with angiogenic markers and poor survival in head and neck squamous cell carcinomaInt J Oncol1710991105200011078794

12. 

S KuraharaM ShinoharaT IkebeS NakamuraM BeppuA HirakiH TakeuchiK ShirasunaExpression of MMPs, MT-MMP, and TIMPs in squamous cell carcinoma of the oral cavity: correlation with tumor invasion and metastasisHead Neck21627638199910.1002/(SICI)1097-0347(199910)21:7%3C627::AID-HED7%3E3.0.CO;2-210487950

13. 

MW RoomiN RoomiT KalinovskyM RathA NiedzwieckiMarked inhibition of growth and invasive parameters of head and neck squamous carcinoma FaDu by a nutrient mixtureIntegr Cancer Ther8168176200910.1177/153473540833463219679626

14. 

M RathL PaulingPlasmin-induced proteolysis and the role of apoprotein(a), lysine and synthetic analogsOrthomolecular Med717231992

15. 

Z SunYH ChenP WangJ ZhangV GurewichP ZhangJN LiuThe blockage of high-affinity lysine binding sites of plasminogen by EACA significantly inhibits prourokinase-induced plasminogen activationBiochem Biophys Acta15961821922002

16. 

S ValcicBN TimmermannDS AlbertsGA WachterM KrutzschJ WymerJM GuillenInhibitory effect of six green tea catechins and caffeine on the growth of four selected human tumor cell linesAnticancer Drugs7461468199610.1097/00001813-199606000-000118826614

17. 

H MukhtarN AhmedTea polyphenols: prevention of cancer and optimizing healthAm J Clin Nutr711698S1702S200010837321

18. 

GY YangJ LiaoK KimEJ YurtowCS YangInhibition of growth and induction of apoptosis in human cancer cell lines by tea polyphenolsCarcinogenesis19611616199810.1093/carcin/19.4.6119600345

19. 

S TaniguchiH FujikiH KobayashiH GoK MiyadoH SadanoR ShimikawaEffect of (−)-epigallocatechin gallate, the main constituent of green tea, on lung metastasis with mouse B16 melanoma cell linesCancer Lett6551541992

20. 

Y HaraGreen tea: Health Benefits and ApplicationsMarcel DekkerNew York200110.1201/9780203907993

21. 

S KawakamiY KageyamaY FujiiK KiharaH OshimaInhibitory effects of N-acetylcysteine on invasion and MMP-9 production of T24 human bladder cancer cellsAnticancer Res21213219200111299737

22. 

M MoriniT CaiMG AluigiDM NoonanL MasielloS De FloroF D’AgostininA AlbiniG FassimaThe role of the thiol N-acetylcysteine in the prevention of tumor invasion and angiogenesisInt J Biol Markers14268271199910669958

23. 

SO YoonMM KimAS ChungInhibitory effects of selenite on invasion of HT1080 tumor cellsJ Biol Chem2762008520092200110.1074/jbc.M10114320011274215

24. 

C MaramagM MenonKC BalajiPG ReddyS LaxmananEffect of vitamin C on prostate cancer cells in vitro: effect on cell number, viability and DNA synthesisProstate32188195199710.1002/(SICI)1097-0045(19970801)32:3%3C188::AID-PROS5%3E3.0.CO;2-H9254898

25. 

KA NaiduRC KarlD CoppolaAntiproliferative and proapoptotic effect of ascorbyl stearate in human pancreatic cancer cells: association with decreased expression of insulin-like growth factor 1 receptorDig Dis Sci48230237200310.1023/A:1021779624971

26. 

WS KohSJ LeeH LeeC ParkMH ParkWS KimSS YoonK ParkSI HongMH ChungCH ParkDifferential effects and transport kinetics of ascorbate derivatives in leukemic cell linesAnticancer Res82487249319989703897

27. 

Q ChenMG EspeyMC KrishnaJB MitchellCP CorpeGR BuettnerE ShacterM LevinePharmacologic ascorbic acid concentrations selectively kill cancer cells: Action as a pro-drug to deliver hydrogen peroxide to tissuesProc Natl Acad Sci USA1021360413609200510.1073/pnas.050639010216157892

28. 

CM KurbacherU WagnerB KolsterPE AndreottiD KrebsHW BrucknerAscorbic acid (vitamin C) improves the antineoplastic activity of doxorubicin, cisplatin and paclitaxel in human breast carcinoma cells in vitroCancer Lett103183189199610.1016/0304-3835(96)04212-78635156

29. 

HM AnthonyCJ SchorahSevere hypovitaminosis C in lung-cancer patients: The utilization of vitamin C in surgical repair and lymphocyte-related host resistanceBr J Cancer46354367198210.1038/bjc.1982.2117126425

30. 

C Núñez MartínA Ortiz de Apodaca y RuizAscorbic acid in the plasma and blood cells of women with breast cancer. The effect of consumption of food with an elevated content of this vitaminNutr Hosp103683721995(In Spanish).

31. 

JP CookeVJ DzauNitric oxide synthase: Role in the genesis of vascular diseaseAnnu Rev Med48489509199710.1146/annurev.med.48.1.4899046979

32. 

MW RoomiV IvanovSP NetkeA NiedzwieckiM RathSerum markers of the liver, heart, and kidney and lipid profile and histopathology in ODS rats treated with nutrient synergyJ AM Coll Nutr22477abs. 862003

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Copy and paste a formatted citation
Spandidos Publications style
Roomi MW, Kalinovsky T, Roomi NW, Niedzwiecki A and Rath M: In vitro and in vivo inhibition of human Fanconi anemia-associated head and neck squamous cell carcinoma by a novel nutrient mixture. Int J Oncol 41: 1996-2004, 2012.
APA
Roomi, M.W., Kalinovsky, T., Roomi, N.W., Niedzwiecki, A., & Rath, M. (2012). In vitro and in vivo inhibition of human Fanconi anemia-associated head and neck squamous cell carcinoma by a novel nutrient mixture. International Journal of Oncology, 41, 1996-2004. https://doi.org/10.3892/ijo.2012.1639
MLA
Roomi, M. W., Kalinovsky, T., Roomi, N. W., Niedzwiecki, A., Rath, M."In vitro and in vivo inhibition of human Fanconi anemia-associated head and neck squamous cell carcinoma by a novel nutrient mixture". International Journal of Oncology 41.6 (2012): 1996-2004.
Chicago
Roomi, M. W., Kalinovsky, T., Roomi, N. W., Niedzwiecki, A., Rath, M."In vitro and in vivo inhibition of human Fanconi anemia-associated head and neck squamous cell carcinoma by a novel nutrient mixture". International Journal of Oncology 41, no. 6 (2012): 1996-2004. https://doi.org/10.3892/ijo.2012.1639
Copy and paste a formatted citation
x
Spandidos Publications style
Roomi MW, Kalinovsky T, Roomi NW, Niedzwiecki A and Rath M: In vitro and in vivo inhibition of human Fanconi anemia-associated head and neck squamous cell carcinoma by a novel nutrient mixture. Int J Oncol 41: 1996-2004, 2012.
APA
Roomi, M.W., Kalinovsky, T., Roomi, N.W., Niedzwiecki, A., & Rath, M. (2012). In vitro and in vivo inhibition of human Fanconi anemia-associated head and neck squamous cell carcinoma by a novel nutrient mixture. International Journal of Oncology, 41, 1996-2004. https://doi.org/10.3892/ijo.2012.1639
MLA
Roomi, M. W., Kalinovsky, T., Roomi, N. W., Niedzwiecki, A., Rath, M."In vitro and in vivo inhibition of human Fanconi anemia-associated head and neck squamous cell carcinoma by a novel nutrient mixture". International Journal of Oncology 41.6 (2012): 1996-2004.
Chicago
Roomi, M. W., Kalinovsky, T., Roomi, N. W., Niedzwiecki, A., Rath, M."In vitro and in vivo inhibition of human Fanconi anemia-associated head and neck squamous cell carcinoma by a novel nutrient mixture". International Journal of Oncology 41, no. 6 (2012): 1996-2004. https://doi.org/10.3892/ijo.2012.1639
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