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Article

Functional analysis of Zyxin in cell migration and invasive potential of oral squamous cell carcinoma cells

Corrigendum in: /10.3892/ijo.2016.3702
  • Authors:
    • Michiyo Yamamura
    • Kazuma Noguchi
    • Yoshiro Nakano
    • Emi Segawa
    • Yusuke Zushi
    • Kazuki Takaoka
    • Hiromitsu Kishimoto
    • Tomoko Hashimoto-Tamaoki
    • Masahiro Urade
  • View Affiliations / Copyright

    Affiliations: Department of Oral and Maxillofacial Surgery, Hyogo College of Medicine, Nishinomiya, Hyogo 663-8501, Japan, Department of Genetics, Hyogo College of Medicine, Nishinomiya, Hyogo 663-8501, Japan
  • Pages: 873-880
    |
    Published online on: January 4, 2013
       https://doi.org/10.3892/ijo.2013.1761
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Abstract

Zyxin is an evolutionarily conserved protein that has been implicated in the regulation of actin assembly and is mainly located at focal adhesions. However, the biological roles of Zyxin in cancer cells are incompletely understood. We analyzed the functions of Zyxin in cell migration and the invasive potential of OSCC. Zyxin expression was examined using eight OSCC cell lines with two different cell morphologies (6 epithelial type and 2 fibroblastic type). To knockdown Zyxin expression, OSCC cells were transfected with Zyxin siRNA and control siRNA. The cell lines were studied by western blot analysis, immunocytochemical analysis and cell migration and invasion assay. Epithelial type OSCC cells showed a high level of E-cadherin expression and a low level of Zyxin expression. N-cadherin as well as Zyxin were strongly expressed in fibroblastic type OSCC cells. Expression levels of LPP and TRIP6, members of the human Zyxin family, did not differ between epithelial type and fibroblastic type. Knockdown of Zyxin expression by siRNA in fibroblastic type OSCC cells was associated with cell morphological changes from spindle (fibroblastic) to polygonal (epithelial) shape and significantly inhibited cell growth as well as cell migration and invasion. Expression levels of Rac1 and Cdc42 were weaker in Zyxin siRNA-treated fibroblastic type OSCC cells than in control siRNA-treated cells, but the expression of RhoA did not differ significantly. Treatment of fibroblastic type OSCC cells with Rac1 inhibitor decreased the expression of Zyxin mRNA and protein. Zyxin is suggested to promote growth, migration and invasiveness of fibroblastic type OSCC cells by upregulating Rac1 and Cdc42.
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Copy and paste a formatted citation
Spandidos Publications style
Yamamura M, Noguchi K, Nakano Y, Segawa E, Zushi Y, Takaoka K, Kishimoto H, Hashimoto-Tamaoki T and Urade M: Functional analysis of Zyxin in cell migration and invasive potential of oral squamous cell carcinoma cells Corrigendum in /10.3892/ijo.2016.3702. Int J Oncol 42: 873-880, 2013.
APA
Yamamura, M., Noguchi, K., Nakano, Y., Segawa, E., Zushi, Y., Takaoka, K. ... Urade, M. (2013). Functional analysis of Zyxin in cell migration and invasive potential of oral squamous cell carcinoma cells Corrigendum in /10.3892/ijo.2016.3702. International Journal of Oncology, 42, 873-880. https://doi.org/10.3892/ijo.2013.1761
MLA
Yamamura, M., Noguchi, K., Nakano, Y., Segawa, E., Zushi, Y., Takaoka, K., Kishimoto, H., Hashimoto-Tamaoki, T., Urade, M."Functional analysis of Zyxin in cell migration and invasive potential of oral squamous cell carcinoma cells Corrigendum in /10.3892/ijo.2016.3702". International Journal of Oncology 42.3 (2013): 873-880.
Chicago
Yamamura, M., Noguchi, K., Nakano, Y., Segawa, E., Zushi, Y., Takaoka, K., Kishimoto, H., Hashimoto-Tamaoki, T., Urade, M."Functional analysis of Zyxin in cell migration and invasive potential of oral squamous cell carcinoma cells Corrigendum in /10.3892/ijo.2016.3702". International Journal of Oncology 42, no. 3 (2013): 873-880. https://doi.org/10.3892/ijo.2013.1761
Copy and paste a formatted citation
x
Spandidos Publications style
Yamamura M, Noguchi K, Nakano Y, Segawa E, Zushi Y, Takaoka K, Kishimoto H, Hashimoto-Tamaoki T and Urade M: Functional analysis of Zyxin in cell migration and invasive potential of oral squamous cell carcinoma cells Corrigendum in /10.3892/ijo.2016.3702. Int J Oncol 42: 873-880, 2013.
APA
Yamamura, M., Noguchi, K., Nakano, Y., Segawa, E., Zushi, Y., Takaoka, K. ... Urade, M. (2013). Functional analysis of Zyxin in cell migration and invasive potential of oral squamous cell carcinoma cells Corrigendum in /10.3892/ijo.2016.3702. International Journal of Oncology, 42, 873-880. https://doi.org/10.3892/ijo.2013.1761
MLA
Yamamura, M., Noguchi, K., Nakano, Y., Segawa, E., Zushi, Y., Takaoka, K., Kishimoto, H., Hashimoto-Tamaoki, T., Urade, M."Functional analysis of Zyxin in cell migration and invasive potential of oral squamous cell carcinoma cells Corrigendum in /10.3892/ijo.2016.3702". International Journal of Oncology 42.3 (2013): 873-880.
Chicago
Yamamura, M., Noguchi, K., Nakano, Y., Segawa, E., Zushi, Y., Takaoka, K., Kishimoto, H., Hashimoto-Tamaoki, T., Urade, M."Functional analysis of Zyxin in cell migration and invasive potential of oral squamous cell carcinoma cells Corrigendum in /10.3892/ijo.2016.3702". International Journal of Oncology 42, no. 3 (2013): 873-880. https://doi.org/10.3892/ijo.2013.1761
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