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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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Article

The inhibitory effect of anthocyanins on Akt on invasion and epithelial-mesenchymal transition is not associated with the anti-EGFR effect of the anthocyanins

  • Authors:
    • Jing Nan Lu
    • Won Sup Lee
    • Min Jeong Kim
    • Jeong Won Yun
    • Ji Hyun Jung
    • Sang Mi Yi
    • Jae-Hoon Jeong
    • Hye Jung Kim
    • Yung Hyun Choi
    • Gon‑Sup Kim
    • Chung Ho Ryu
    • Sung Chul Shin
  • View Affiliations / Copyright

    Affiliations: Department of Internal Medicine, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju, Republic of Korea , Research Center for Radiotherapy, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea, Department of Pharmacology, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju, Republic of Korea , Department of Biochemistry, Dongeui University College of Oriental Medicine and Anti-Aging Research Center and Blue-Bio Industry RIC, Dongeui University, Busan, Republic of Korea, School of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of Korea, Division of Applied Life Science (BK 21 Program), Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, Republic of Korea, Department of Chemistry and Research Institute of Life Science, Gyeongsang National University, Jinju, Republic of Korea
  • Pages: 1756-1766
    |
    Published online on: February 27, 2014
       https://doi.org/10.3892/ijo.2014.2315
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Abstract

Evidence suggests that anthocyanins inhibit EGFR and Akt activity. However, it is still unknown whether the inhibitory effect of anthocyanins on Akt is associated with the anti-EGFR effect. The effect of anthocyanins on epithelial-mesenchymal transition (EMT) has not been extensively studied. Therefore, we investigated the effects of anthocyanins from fruits of Vitis coignetiae Pulliat (AIMs) on EGF-induced EMT and the underlying molecular mechanisms. AIMs suppressed the invasion of A549 cells in a dose-dependent manner. AIMs inhibited the phosphorylation of Akt and EGFR, but the inhibitory effect on Akt was not derived from EGFR. EGF re-induced Akt phosphorylation at Thr308 in the AIM-treated cells, but not Akt phosphorylation at Ser473. AIMs also inhibited EMT of cancer cells. AIMs inhibited glycogen synthase kinase-3β phosphorylation and β-catenin expression that are invovled in EMT. We confirmed these findings with transforming growth factor (TGF)-β. In conclusion, these data suggest that the inhibitory effect of AIMs on Akt activity is independent of EGFR, and that AIMs suppressed invasion and migration at least in part by suppressing EMT by inhibiting Akt activity as well as EGFR. This study provides evidence that AIMs may have anticancer effects on human cancer cells.
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Copy and paste a formatted citation
Spandidos Publications style
Lu JN, Lee WS, Kim MJ, Yun JW, Jung JH, Yi SM, Jeong J, Kim HJ, Choi YH, Kim GS, Kim GS, et al: The inhibitory effect of anthocyanins on Akt on invasion and epithelial-mesenchymal transition is not associated with the anti-EGFR effect of the anthocyanins. Int J Oncol 44: 1756-1766, 2014.
APA
Lu, J.N., Lee, W.S., Kim, M.J., Yun, J.W., Jung, J.H., Yi, S.M. ... Shin, S.C. (2014). The inhibitory effect of anthocyanins on Akt on invasion and epithelial-mesenchymal transition is not associated with the anti-EGFR effect of the anthocyanins. International Journal of Oncology, 44, 1756-1766. https://doi.org/10.3892/ijo.2014.2315
MLA
Lu, J. N., Lee, W. S., Kim, M. J., Yun, J. W., Jung, J. H., Yi, S. M., Jeong, J., Kim, H. J., Choi, Y. H., Kim, G., Ryu, C. H., Shin, S. C."The inhibitory effect of anthocyanins on Akt on invasion and epithelial-mesenchymal transition is not associated with the anti-EGFR effect of the anthocyanins". International Journal of Oncology 44.5 (2014): 1756-1766.
Chicago
Lu, J. N., Lee, W. S., Kim, M. J., Yun, J. W., Jung, J. H., Yi, S. M., Jeong, J., Kim, H. J., Choi, Y. H., Kim, G., Ryu, C. H., Shin, S. C."The inhibitory effect of anthocyanins on Akt on invasion and epithelial-mesenchymal transition is not associated with the anti-EGFR effect of the anthocyanins". International Journal of Oncology 44, no. 5 (2014): 1756-1766. https://doi.org/10.3892/ijo.2014.2315
Copy and paste a formatted citation
x
Spandidos Publications style
Lu JN, Lee WS, Kim MJ, Yun JW, Jung JH, Yi SM, Jeong J, Kim HJ, Choi YH, Kim GS, Kim GS, et al: The inhibitory effect of anthocyanins on Akt on invasion and epithelial-mesenchymal transition is not associated with the anti-EGFR effect of the anthocyanins. Int J Oncol 44: 1756-1766, 2014.
APA
Lu, J.N., Lee, W.S., Kim, M.J., Yun, J.W., Jung, J.H., Yi, S.M. ... Shin, S.C. (2014). The inhibitory effect of anthocyanins on Akt on invasion and epithelial-mesenchymal transition is not associated with the anti-EGFR effect of the anthocyanins. International Journal of Oncology, 44, 1756-1766. https://doi.org/10.3892/ijo.2014.2315
MLA
Lu, J. N., Lee, W. S., Kim, M. J., Yun, J. W., Jung, J. H., Yi, S. M., Jeong, J., Kim, H. J., Choi, Y. H., Kim, G., Ryu, C. H., Shin, S. C."The inhibitory effect of anthocyanins on Akt on invasion and epithelial-mesenchymal transition is not associated with the anti-EGFR effect of the anthocyanins". International Journal of Oncology 44.5 (2014): 1756-1766.
Chicago
Lu, J. N., Lee, W. S., Kim, M. J., Yun, J. W., Jung, J. H., Yi, S. M., Jeong, J., Kim, H. J., Choi, Y. H., Kim, G., Ryu, C. H., Shin, S. C."The inhibitory effect of anthocyanins on Akt on invasion and epithelial-mesenchymal transition is not associated with the anti-EGFR effect of the anthocyanins". International Journal of Oncology 44, no. 5 (2014): 1756-1766. https://doi.org/10.3892/ijo.2014.2315
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