Overexpression of integrin αv facilitates proliferation and invasion of oral squamous cell carcinoma cells via mek/erk signaling pathway that is activated by interaction of integrin αvβ8 with type Ⅰ collagen

  • Authors:
    • Yasutaka Hayashido
    • Hisataka Kitano
    • Taishi Sakaue
    • Takahiko  Fujii
    • Mirei Suematsu
    • Shigeru Sakurai
    • Tetsuji Okamoto
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  • Published online on: September 4, 2014     https://doi.org/10.3892/ijo.2014.2642
  • Pages: 1875-1882
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Abstract

To examine the role of integrin αv subunit in the progression of squamous cell carcinoma (SCC), oral SCC cells were stably transfected with integrin αv cDNA. Integrin αv transfectants exhibited the enhancement of proliferation on type Ⅰ collagen, and seemed to have a high ability to invade type Ⅰ collagen gel. Overexpression of integrin αv led to rapid phosphorylation of focal adhesion kinase (FAK), mitogen‑activated protein kinase kinase 1/2 (MEK1/2) and extracellular signal‑regulated kinase 1/2 (ERK1/2) in SCC cells on type Ⅰ collagen. The downregulation of integrin β8 in integrin αv transfectants by its specific antisense oligonucleotide led to a decrease in type Ⅰ collagen‑stimulated activation of FAK and the MEK/ERK signaling pathway, and also suppressed the proliferation on type Ⅰ collagen and the invasiveness into type Ⅰ collagen gel. Moreover, the expression of integrin β8 was induced following transfection with integrin αv cDNA. These results indicated that the overexpression of integrin αv induces integrin αvβ8 heterodimer formation and the binding of integrin αvβ8 to type Ⅰ collagen might enhance the proliferation and invasion of SCC cells via the activation of the MEK/ERK signaling pathway.
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November 2014
Volume 45 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Hayashido, Y., Kitano, H., Sakaue, T., Fujii, T., Suematsu, M., Sakurai, S., & Okamoto, T. (2014). Overexpression of integrin αv facilitates proliferation and invasion of oral squamous cell carcinoma cells via mek/erk signaling pathway that is activated by interaction of integrin αvβ8 with type Ⅰ collagen. International Journal of Oncology, 45, 1875-1882. https://doi.org/10.3892/ijo.2014.2642
MLA
Hayashido, Y., Kitano, H., Sakaue, T., Fujii, T., Suematsu, M., Sakurai, S., Okamoto, T."Overexpression of integrin αv facilitates proliferation and invasion of oral squamous cell carcinoma cells via mek/erk signaling pathway that is activated by interaction of integrin αvβ8 with type Ⅰ collagen". International Journal of Oncology 45.5 (2014): 1875-1882.
Chicago
Hayashido, Y., Kitano, H., Sakaue, T., Fujii, T., Suematsu, M., Sakurai, S., Okamoto, T."Overexpression of integrin αv facilitates proliferation and invasion of oral squamous cell carcinoma cells via mek/erk signaling pathway that is activated by interaction of integrin αvβ8 with type Ⅰ collagen". International Journal of Oncology 45, no. 5 (2014): 1875-1882. https://doi.org/10.3892/ijo.2014.2642