Effects of genistein supplementation on genome‑wide DNA methylation and gene expression in patients with localized prostate cancer

  • Authors:
    • Birdal Bilir
    • Nitya V. Sharma
    • Jeongseok Lee
    • Bato Hammarstrom
    • Aud Svindland
    • Omer Kucuk
    • Carlos S. Moreno
  • View Affiliations

  • Published online on: May 24, 2017     https://doi.org/10.3892/ijo.2017.4017
  • Pages:223-234
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Abstract

Epidemiological studies have shown that dietary compounds have significant effects on prostate carcinogenesis. Among dietary agents, genistein, the major isoflavone in soybean, is of particular interest because high consumption of soy products has been associated with a low incidence of prostate cancer, suggesting a preventive role of genistein in prostate cancer. In spite of numerous studies to understand the effects of genistein on prostate cancer, the mechanisms of action have not been fully elucidated. We investigated the differences in methylation and gene expression levels of prostate specimens from a clinical trial of genistein supplementation prior to prostatectomy using Illumina HumanMethylation450 and Illumina HumanHT-12 v4 Expression BeadChip Microarrays. The present study was a randomized, placebo-controlled, double-blind clinical trial on Norwegian patients who received 30 mg genistein or placebo capsules daily for 3-6 weeks before prostatectomy. Gene expression changes were validated by quantitative PCR (qPCR). Whole genome methylation and expression profiling identified differentially methylated sites and expressed genes between placebo and genistein groups. Differentially regulated genes were involved in developmental processes, stem cell markers, proliferation and transcriptional regulation. Enrichment analysis suggested overall reduction in MYC activity and increased PTEN activity in genistein-treated patients. These findings highlight the effects of genistein on global changes in gene expression in prostate cancer and its effects on molecular pathways involved in prostate tumorigenesis.

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July 2017
Volume 51 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

2016 Impact Factor: 3.079
Ranked #33/217 Oncology
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APA
Bilir, B., Sharma, N.V., Lee, J., Hammarstrom, B., Svindland, A., Kucuk, O., & Moreno, C.S. (2017). Effects of genistein supplementation on genome‑wide DNA methylation and gene expression in patients with localized prostate cancer. International Journal of Oncology, 51, 223-234. https://doi.org/10.3892/ijo.2017.4017
MLA
Bilir, B., Sharma, N. V., Lee, J., Hammarstrom, B., Svindland, A., Kucuk, O., Moreno, C. S."Effects of genistein supplementation on genome‑wide DNA methylation and gene expression in patients with localized prostate cancer". International Journal of Oncology 51.1 (2017): 223-234.
Chicago
Bilir, B., Sharma, N. V., Lee, J., Hammarstrom, B., Svindland, A., Kucuk, O., Moreno, C. S."Effects of genistein supplementation on genome‑wide DNA methylation and gene expression in patients with localized prostate cancer". International Journal of Oncology 51, no. 1 (2017): 223-234. https://doi.org/10.3892/ijo.2017.4017