Cinnamaldehyde/chemotherapeutic agents interaction and drug-metabolizing genes in colorectal cancer

  • Authors:
    • Chen Yu
    • Shen-Lin Liu
    • Ming-Hao Qi
    • Xi Zou
  • View Affiliations

  • Published online on: November 26, 2013     https://doi.org/10.3892/mmr.2013.1830
  • Pages: 669-676
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Abstract

Cinnamaldehyde is an active monomer isolated from the stem bark of Cinnamomum cassia, a traditional oriental medicinal herb, which is known to possess marked antitumor effects in vitro and in vivo. The aim of the present study was to examine the potential advantages of using cinnamaldehyde in combination with chemotherapeutic agents commonly used in colorectal carcinoma (CRC) therapy, as well as to investigate the effect of cinnamaldehyde on chemotherapeutic-associated gene expression. The synergistic interaction of cinnamaldehyde and chemotherapeutic agents on human CRC HT-29 and LoVo cells was evaluated using the combination index (CI) method. The double staining with Annexin V conjugated to fluorescein-isothiocyanate and phosphatidylserine was employed for apoptosis detection. The expression of drug-metabolizing genes, including excision repair cross‑complementing 1 (ERCC1), orotate phosphoribosyltransferase (OPRT), thymidylate synthase (TS), breast cancer susceptibility gene 1 (BRCA1) and topoisomerase 1 (TOPO1), all in HT-29 and LoVo cells, with or without the addition of cinnamaldehyde, was examined by quantitative polymerase chain reaction (PCR). Cinnamaldehyde had a synergistic effect on the chemotherapeutic agents cytotoxicity in HT-29 and LoVo cells. In addition, cinnamaldehyde suppressed BRCA1, TOPO1, ERCC1 and TS mRNA expression, except for OPRT expression, which was markedly upregulated. Our findings indicate that cinnamaldehyde appears to be a promising candidate as an adjuvant in combination therapy with 5-fluorouracil (5-FU) and oxaliplatin (OXA), two chemotherapeutic agents used in CRC treatment. The possible mechanisms of its action may involve the regulation of drug‑metabolizing genes.

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2014-February
Volume 9 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
Yu C, Liu S, Qi M and Zou X: Cinnamaldehyde/chemotherapeutic agents interaction and drug-metabolizing genes in colorectal cancer. Mol Med Rep 9: 669-676, 2014.
APA
Yu, C., Liu, S., Qi, M., & Zou, X. (2014). Cinnamaldehyde/chemotherapeutic agents interaction and drug-metabolizing genes in colorectal cancer. Molecular Medicine Reports, 9, 669-676. https://doi.org/10.3892/mmr.2013.1830
MLA
Yu, C., Liu, S., Qi, M., Zou, X."Cinnamaldehyde/chemotherapeutic agents interaction and drug-metabolizing genes in colorectal cancer". Molecular Medicine Reports 9.2 (2014): 669-676.
Chicago
Yu, C., Liu, S., Qi, M., Zou, X."Cinnamaldehyde/chemotherapeutic agents interaction and drug-metabolizing genes in colorectal cancer". Molecular Medicine Reports 9, no. 2 (2014): 669-676. https://doi.org/10.3892/mmr.2013.1830