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Article

Identification of miR‑130b as an oncogene in renal cell carcinoma

  • Authors:
    • Yifan Li
    • Duqun Chen
    • Yuchi Li
    • Lu Jin
    • Jiaju Liu
    • Zhengming Su
    • Zhengyu Qi
    • Min Shi
    • Zhimao Jiang
    • Yaoting Gui
    • Shangqi Yang
    • Xiangming Mao
    • Yongqing Lai
  • View Affiliations / Copyright

    Affiliations: Department of Urology, Peking University Shenzhen Hospital, Shenzhen PKU‑HKUST Medical Center, Shenzhen, Guangdong 518036, P.R. China, Department of Urology, Anhui Medical University, Hefei, Anhui 230032, P.R. China, Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen PKU‑HKUST Medical Center, Shenzhen, Guangdong 518036, P.R. China
  • Pages: 1902-1908
    |
    Published online on: December 30, 2015
       https://doi.org/10.3892/mmr.2015.4744
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Abstract

Renal cell carcinoma (RCC) is the most common type of renal tumor, which has a poor prognosis. Improvements in understanding the underlying molecular biology of RCC has led to systemic treatments, which have markedly improved patient outcomes. Therefore, it is necessary and worthwhile to identify novel biomarkers for RCC. MicroRNAs (miRNAs) have been found to be important in a wide range of biological and pathological processes, including cell differentiation, migration, growth, proliferation, apoptosis and metabolism. Aberrant expression of miRNA‑130b has previously been reported in tumors, however, its role in RCC remains to be elucidated. In the present study, the upregulation of miR‑130b was observed in RCC tissues and cell lines using reverse transcription‑quantitative polymerase chain reaction analysis, which was consistent with previous microRNA profiling in RCC. Furthermore, the effects of miR‑130b on cell migration, proliferation and apoptosis were examined using a wound scratch assay, an MTT assay and flow cytometric analysis, respectively. The results demonstrated that the downregulation of miR‑130b by a synthesized inhibitor inhibited cell migration, suppressed cell proliferation and induced RCC cell apoptosis. The present study was the first, to the best of our knowledge, to suggest that miR‑130b may be a promising biomarker for diagnosis and a therapeutic target for the treatment of RCC. Further investigations are required to examine the roles and target genes of miR‑130b in RCC.
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Copy and paste a formatted citation
Spandidos Publications style
Li Y, Chen D, Li Y, Jin L, Liu J, Su Z, Qi Z, Shi M, Jiang Z, Gui Y, Gui Y, et al: Identification of miR‑130b as an oncogene in renal cell carcinoma. Mol Med Rep 13: 1902-1908, 2016.
APA
Li, Y., Chen, D., Li, Y., Jin, L., Liu, J., Su, Z. ... Lai, Y. (2016). Identification of miR‑130b as an oncogene in renal cell carcinoma. Molecular Medicine Reports, 13, 1902-1908. https://doi.org/10.3892/mmr.2015.4744
MLA
Li, Y., Chen, D., Li, Y., Jin, L., Liu, J., Su, Z., Qi, Z., Shi, M., Jiang, Z., Gui, Y., Yang, S., Mao, X., Lai, Y."Identification of miR‑130b as an oncogene in renal cell carcinoma". Molecular Medicine Reports 13.2 (2016): 1902-1908.
Chicago
Li, Y., Chen, D., Li, Y., Jin, L., Liu, J., Su, Z., Qi, Z., Shi, M., Jiang, Z., Gui, Y., Yang, S., Mao, X., Lai, Y."Identification of miR‑130b as an oncogene in renal cell carcinoma". Molecular Medicine Reports 13, no. 2 (2016): 1902-1908. https://doi.org/10.3892/mmr.2015.4744
Copy and paste a formatted citation
x
Spandidos Publications style
Li Y, Chen D, Li Y, Jin L, Liu J, Su Z, Qi Z, Shi M, Jiang Z, Gui Y, Gui Y, et al: Identification of miR‑130b as an oncogene in renal cell carcinoma. Mol Med Rep 13: 1902-1908, 2016.
APA
Li, Y., Chen, D., Li, Y., Jin, L., Liu, J., Su, Z. ... Lai, Y. (2016). Identification of miR‑130b as an oncogene in renal cell carcinoma. Molecular Medicine Reports, 13, 1902-1908. https://doi.org/10.3892/mmr.2015.4744
MLA
Li, Y., Chen, D., Li, Y., Jin, L., Liu, J., Su, Z., Qi, Z., Shi, M., Jiang, Z., Gui, Y., Yang, S., Mao, X., Lai, Y."Identification of miR‑130b as an oncogene in renal cell carcinoma". Molecular Medicine Reports 13.2 (2016): 1902-1908.
Chicago
Li, Y., Chen, D., Li, Y., Jin, L., Liu, J., Su, Z., Qi, Z., Shi, M., Jiang, Z., Gui, Y., Yang, S., Mao, X., Lai, Y."Identification of miR‑130b as an oncogene in renal cell carcinoma". Molecular Medicine Reports 13, no. 2 (2016): 1902-1908. https://doi.org/10.3892/mmr.2015.4744
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