Allicin attenuates H2O2‑induced cytotoxicity in retinal pigmented epithelial cells by regulating the levels of reactive oxygen species

  • Authors:
    • Gerile Tu
    • Yu‑Feng Zhang
    • Wei Wei
    • Langen Li
    • Yanmei Zhang
    • Jia Yang
    • Yiqiao Xing
  • View Affiliations

  • Published online on: January 18, 2016     https://doi.org/10.3892/mmr.2016.4797
  • Pages: 2320-2326
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Abstract

Retinal pigmented epithelial cell (RPE) oxidative stress is known to have a vital role in the etiology of age‑related macular degeneration (AMD). The present study aimed to investigate whether allicin, a natural product with antioxidant activity, was able to protect RPEs (ARPE‑19) from hydrogen peroxide (H2O2)‑induced damage, and to determine the underlying mechanisms. The 3-(4,5-dimethylthiazol-2-yl)-2,5‑diphenyl tetrazolium bromide assay was used to determine cellular viability, and reactive oxygen species (ROS) were detected using a ROS Assay kit. The results demonstrated that allicin was able to protect ARPE‑19 cells from H2O2‑induced damage in a dose‑dependent manner. In addition, allicin attenuated oxidative stress by reducing the levels of intracellular ROS and malondialdehyde (MDA), and enhancing the glutathione/glutathione disulfide (GSSG) ratio. With regards to the underlying mechanism, allicin was able to markedly modulate the expression levels of ROS‑associated enzymes, including superoxide dismutase, NADPH oxidase 4 and NAD(P)H dehydrogenase quinone 1, and elevate the activity of nuclear factor erythroid 2‑related factor 2 in the H2O2‑stimulated ARPE‑19 cells. These results suggested that allicin may exert protective effects against H2O2‑induced cytotoxicity in RPEs via ROS regulation.
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March-2016
Volume 13 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Tu G, Zhang YF, Wei W, Li L, Zhang Y, Yang J and Xing Y: Allicin attenuates H2O2‑induced cytotoxicity in retinal pigmented epithelial cells by regulating the levels of reactive oxygen species. Mol Med Rep 13: 2320-2326, 2016
APA
Tu, G., Zhang, Y., Wei, W., Li, L., Zhang, Y., Yang, J., & Xing, Y. (2016). Allicin attenuates H2O2‑induced cytotoxicity in retinal pigmented epithelial cells by regulating the levels of reactive oxygen species. Molecular Medicine Reports, 13, 2320-2326. https://doi.org/10.3892/mmr.2016.4797
MLA
Tu, G., Zhang, Y., Wei, W., Li, L., Zhang, Y., Yang, J., Xing, Y."Allicin attenuates H2O2‑induced cytotoxicity in retinal pigmented epithelial cells by regulating the levels of reactive oxygen species". Molecular Medicine Reports 13.3 (2016): 2320-2326.
Chicago
Tu, G., Zhang, Y., Wei, W., Li, L., Zhang, Y., Yang, J., Xing, Y."Allicin attenuates H2O2‑induced cytotoxicity in retinal pigmented epithelial cells by regulating the levels of reactive oxygen species". Molecular Medicine Reports 13, no. 3 (2016): 2320-2326. https://doi.org/10.3892/mmr.2016.4797