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Differential changes in Neuregulin-1 signaling in major brain regions in a lipopolysaccharide-induced neuroinflammation mouse model

  • Authors:
    • Zhai Yang
    • Qiong Jiang
    • Shuang‑Xi Chen
    • Cheng‑Liang Hu
    • Hui‑Fan Shen
    • Pei‑Zhi Huang
    • Jun‑Ping Xu
    • Jin‑Ping Mei
    • Ben‑Ping Zhang
    • Wei‑Jiang Zhao
  • View Affiliations / Copyright

    Affiliations: Center for Neuroscience, Shantou University Medical College, Shantou, Guangdong 515041, P.R. China, Graduate School, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, P.R. China, Department of Neurology, The 2nd Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 790-796
    |
    Published online on: May 23, 2016
       https://doi.org/10.3892/mmr.2016.5325
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Abstract

Neuregulin 1 (Nrg1) is involved in multiple biological processes in the nervous system. The present study investigated changes in Nrg1 signaling in the major brain regions of mice subjected to lipopolysaccharide (LPS)-induced neuroinflammation. At 24 h post‑intraperitoneal injection of LPS, mouse brain tissues, including tissues from the cortex, striatum, hippocampus and hypothalamus, were collected. Reverse transcription‑polymerase chain reaction was used to determine the expression of Nrg1 and its receptors, Neu and ErbB4, at the mRNA level. Western blotting was performed to determine the levels of these proteins and the protein levels of phosphorylated extracellular signal-regulated kinases (Erk)1/2 and Akt1. Immunohistochemical staining was utilized to detect the levels of pNeu and pErbB4 in these regions. LPS successfully induced sites of neuroinflammation in these regions, in which changes in Nrg1, Neu and ErbB4 at the mRNA and protein levels were identified compared with controls. LPS induced a reduction in pNeu and pErbB4 in the striatum and hypothalamus, although marginally increased pErbB4 levels were found in the hippocampus. LPS increased the overall phosphorylation of Src but this effect was reduced in the hypothalamus. Moreover, increased phosphorylation of Akt1 was found in the striatum and hippocampus. These data suggest diverse roles for Nrg1 signaling in these regions during the process of neuroinflammation.
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Copy and paste a formatted citation
Spandidos Publications style
Yang Z, Jiang Q, Chen SX, Hu CL, Shen HF, Huang PZ, Xu JP, Mei JP, Zhang BP, Zhao WJ, Zhao WJ, et al: Differential changes in Neuregulin-1 signaling in major brain regions in a lipopolysaccharide-induced neuroinflammation mouse model. Mol Med Rep 14: 790-796, 2016.
APA
Yang, Z., Jiang, Q., Chen, S., Hu, C., Shen, H., Huang, P. ... Zhao, W. (2016). Differential changes in Neuregulin-1 signaling in major brain regions in a lipopolysaccharide-induced neuroinflammation mouse model. Molecular Medicine Reports, 14, 790-796. https://doi.org/10.3892/mmr.2016.5325
MLA
Yang, Z., Jiang, Q., Chen, S., Hu, C., Shen, H., Huang, P., Xu, J., Mei, J., Zhang, B., Zhao, W."Differential changes in Neuregulin-1 signaling in major brain regions in a lipopolysaccharide-induced neuroinflammation mouse model". Molecular Medicine Reports 14.1 (2016): 790-796.
Chicago
Yang, Z., Jiang, Q., Chen, S., Hu, C., Shen, H., Huang, P., Xu, J., Mei, J., Zhang, B., Zhao, W."Differential changes in Neuregulin-1 signaling in major brain regions in a lipopolysaccharide-induced neuroinflammation mouse model". Molecular Medicine Reports 14, no. 1 (2016): 790-796. https://doi.org/10.3892/mmr.2016.5325
Copy and paste a formatted citation
x
Spandidos Publications style
Yang Z, Jiang Q, Chen SX, Hu CL, Shen HF, Huang PZ, Xu JP, Mei JP, Zhang BP, Zhao WJ, Zhao WJ, et al: Differential changes in Neuregulin-1 signaling in major brain regions in a lipopolysaccharide-induced neuroinflammation mouse model. Mol Med Rep 14: 790-796, 2016.
APA
Yang, Z., Jiang, Q., Chen, S., Hu, C., Shen, H., Huang, P. ... Zhao, W. (2016). Differential changes in Neuregulin-1 signaling in major brain regions in a lipopolysaccharide-induced neuroinflammation mouse model. Molecular Medicine Reports, 14, 790-796. https://doi.org/10.3892/mmr.2016.5325
MLA
Yang, Z., Jiang, Q., Chen, S., Hu, C., Shen, H., Huang, P., Xu, J., Mei, J., Zhang, B., Zhao, W."Differential changes in Neuregulin-1 signaling in major brain regions in a lipopolysaccharide-induced neuroinflammation mouse model". Molecular Medicine Reports 14.1 (2016): 790-796.
Chicago
Yang, Z., Jiang, Q., Chen, S., Hu, C., Shen, H., Huang, P., Xu, J., Mei, J., Zhang, B., Zhao, W."Differential changes in Neuregulin-1 signaling in major brain regions in a lipopolysaccharide-induced neuroinflammation mouse model". Molecular Medicine Reports 14, no. 1 (2016): 790-796. https://doi.org/10.3892/mmr.2016.5325
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