Gastrodin inhibits high glucose‑induced human retinal endothelial cell apoptosis by regulating the SIRT1/TLR4/NF‑κBp65 signaling pathway

  • Authors:
    • Tong‑He Zhang
    • Chun‑Mei Huang
    • Xue Gao
    • Jia‑Wei Wang
    • Lin‑Lin Hao
    • Qiang Ji
  • View Affiliations

  • Published online on: April 3, 2018     https://doi.org/10.3892/mmr.2018.8841
  • Pages: 7774-7780
Metrics: HTML 0 views | PDF 0 views     Cited By (CrossRef): 0 citations

Abstract

Diabetic retinopathy (DR), one of the most common complications of late‑phase diabetes, is associated with the ectopic apoptosis of microvascular cells. Gastrodin, a phenolic glucoside derived from Gastrodia elata Blume, has been reported to have antioxidant and anti‑inflammation activities. The aim of the present study was to investigate the effects of gastrodin on high glucose (HG)‑induced human retinal endothelial cell (HREC) injury and its underlying mechanism. The results demonstrated that HG induced cell apoptosis in HRECs, which was accompanied by increased levels of reactive oxygen species production. Gastrodin treatment significantly alleviated HG‑induced apoptosis and oxidative stress. Furthermore, HG stimulation decreased the levels of SIRT1, which was accompanied by an increase in Toll‑like receptor 4 (TLR4) expression and the levels of phosphorylated nuclear factor (NF)‑κBp65. However, the administration of gastrodin significantly inhibited the activation of the sirtuin 1 (SIRT1)/TLR4/NF‑κBp65 signaling pathway in HRECs exposed to HG. Collectively, the present study demonstrated that gastrodin may be effective against HG‑induced apoptosis and its action may be exerted through the regulation of the SIRT1/TLR4/NF‑κBp65 signaling pathway.

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June 2018
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APA
Zhang, T., Huang, C., Gao, X., Wang, J., Hao, L., & Ji, Q. (2018). Gastrodin inhibits high glucose‑induced human retinal endothelial cell apoptosis by regulating the SIRT1/TLR4/NF‑κBp65 signaling pathway. Molecular Medicine Reports, 17, 7774-7780. https://doi.org/10.3892/mmr.2018.8841
MLA
Zhang, T., Huang, C., Gao, X., Wang, J., Hao, L., Ji, Q."Gastrodin inhibits high glucose‑induced human retinal endothelial cell apoptosis by regulating the SIRT1/TLR4/NF‑κBp65 signaling pathway". Molecular Medicine Reports 17.6 (2018): 7774-7780.
Chicago
Zhang, T., Huang, C., Gao, X., Wang, J., Hao, L., Ji, Q."Gastrodin inhibits high glucose‑induced human retinal endothelial cell apoptosis by regulating the SIRT1/TLR4/NF‑κBp65 signaling pathway". Molecular Medicine Reports 17, no. 6 (2018): 7774-7780. https://doi.org/10.3892/mmr.2018.8841