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Article Open Access

Apigenin suppresses the apoptosis of H9C2 rat cardiomyocytes subjected to myocardial ischemia‑reperfusion injury via upregulation of the PI3K/Akt pathway

  • Authors:
    • Zhengwen Zhou
    • Yue Zhang
    • Luning Lin
    • Jianmei Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Electrocardiogram Diagnosis, Zhejiang Hospital, Hangzhou, Zhejiang 310013, P.R. China, Department of Cardiovasology, Zhejiang Hospital, Hangzhou, Zhejiang 310013, P.R. China, College of Pharmaceutical Science, Zhejiang University of Traditional Chinese Medicine, Hangzhou, Zhejiang 310013, P.R. China, Department of Cardiac Rehabilitation, Zhejiang Hospital, Hangzhou, Zhejiang 310013, P.R. China
    Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1560-1570
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    Published online on: May 31, 2018
       https://doi.org/10.3892/mmr.2018.9115
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Abstract

Apigenin, a flavonoid with multiple physiological and pharmacological activities, is associated with the prevention of cardiovascular diseases. The present study aimed to examine the roles and mechanisms of apigenin in the apoptosis of H9C2 rat cardiomyocytes, which were subjected to myocardial ischemia‑reperfusion (MI/R) injury. Cell viability, reactive oxygen species (ROS), mitochondrial membrane potential (MMP) and cellular apoptosis were evaluated using cell counting kit‑8 assays and flow cytometry. The content/activity of oxidative stress markers was determined using commercial kits. Western blot analysis and reverse transcription‑quantitative polymerase chain reaction assays were used to measure protein and mRNA expression, respectively. The results demonstrated that apigenin had limited cytotoxicity on the viability of H9C2 rat cardiomyocytes. Apigenin reduced the oxidative stress, ROS production and cellular apoptotic capacity of MI/R‑induced H9C2 cells. Apigenin additionally increased the MMP level of MI/R‑induced H9C2 cells. Furthermore, apigenin modulated apoptosis‑associated protein expression and phosphatidylinositol 3'-kinase (PI3K)/RAC‑α serine/threonine‑protein kinase (Akt) signaling in MI/R‑induced H9C2 cells. Treatment with LY294002 reversed the anti‑apoptotic effect of apigenin. In conclusion, apigenin suppressed the apoptosis of H9C2 cells that were subjected to MI/R injury by activating the PI3K/Akt pathway. It was suggested that apigenin may be effective as an MI/R therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Zhou Z, Zhang Y, Lin L and Zhou J: Apigenin suppresses the apoptosis of H9C2 rat cardiomyocytes subjected to myocardial ischemia‑reperfusion injury via upregulation of the PI3K/Akt pathway. Mol Med Rep 18: 1560-1570, 2018.
APA
Zhou, Z., Zhang, Y., Lin, L., & Zhou, J. (2018). Apigenin suppresses the apoptosis of H9C2 rat cardiomyocytes subjected to myocardial ischemia‑reperfusion injury via upregulation of the PI3K/Akt pathway. Molecular Medicine Reports, 18, 1560-1570. https://doi.org/10.3892/mmr.2018.9115
MLA
Zhou, Z., Zhang, Y., Lin, L., Zhou, J."Apigenin suppresses the apoptosis of H9C2 rat cardiomyocytes subjected to myocardial ischemia‑reperfusion injury via upregulation of the PI3K/Akt pathway". Molecular Medicine Reports 18.2 (2018): 1560-1570.
Chicago
Zhou, Z., Zhang, Y., Lin, L., Zhou, J."Apigenin suppresses the apoptosis of H9C2 rat cardiomyocytes subjected to myocardial ischemia‑reperfusion injury via upregulation of the PI3K/Akt pathway". Molecular Medicine Reports 18, no. 2 (2018): 1560-1570. https://doi.org/10.3892/mmr.2018.9115
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou Z, Zhang Y, Lin L and Zhou J: Apigenin suppresses the apoptosis of H9C2 rat cardiomyocytes subjected to myocardial ischemia‑reperfusion injury via upregulation of the PI3K/Akt pathway. Mol Med Rep 18: 1560-1570, 2018.
APA
Zhou, Z., Zhang, Y., Lin, L., & Zhou, J. (2018). Apigenin suppresses the apoptosis of H9C2 rat cardiomyocytes subjected to myocardial ischemia‑reperfusion injury via upregulation of the PI3K/Akt pathway. Molecular Medicine Reports, 18, 1560-1570. https://doi.org/10.3892/mmr.2018.9115
MLA
Zhou, Z., Zhang, Y., Lin, L., Zhou, J."Apigenin suppresses the apoptosis of H9C2 rat cardiomyocytes subjected to myocardial ischemia‑reperfusion injury via upregulation of the PI3K/Akt pathway". Molecular Medicine Reports 18.2 (2018): 1560-1570.
Chicago
Zhou, Z., Zhang, Y., Lin, L., Zhou, J."Apigenin suppresses the apoptosis of H9C2 rat cardiomyocytes subjected to myocardial ischemia‑reperfusion injury via upregulation of the PI3K/Akt pathway". Molecular Medicine Reports 18, no. 2 (2018): 1560-1570. https://doi.org/10.3892/mmr.2018.9115
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