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Article

Sevoflurane attenuates ventilator‑induced lung injury by regulating c‑PLA2 expression

  • Authors:
    • Yong Yang
    • Wen‑Fa Wang
    • Yan‑Hua Li
    • Li‑Sha Li
    • Xin Guo
    • Rui Liu
  • View Affiliations / Copyright

    Affiliations: Experimental Center of Medical Function, Kunming Medical University, Kunming, Yunnan 650031, P.R. China, Department of Anesthesiology, The People's Hospital of Chuxiong Yi Autonomous Prefecture, Chuxiong, Yunnan 675000, P.R. China, Department of Anesthesiology, The First People's Hospital of Yunnan Province, Kunming, Yunnan 650032, P.R. China
  • Pages: 2923-2928
    |
    Published online on: July 3, 2018
       https://doi.org/10.3892/mmr.2018.9243
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Abstract

The aim of the present study was to investigate the potential role of club cell secretory protein (CCSP), an endogenous modulator, in reducing pulmonary inflammation induced by sevoflurane following one‑lung ventilation (OLV). Healthy Japanese white rabbits were randomly assigned to six groups: Sham‑operated group (group S); respiratory management of OLV group (group O); OLV + sevoflurane treated group (group OF), club cells exfoliated + sham‑operated group (group NA), club cells exfoliated + OLV group (group NAO); and club cells exfoliated + OLV + sevoflurane treated group (group NAOF). At the end of the experimental observation, all animals in the different groups were sacrificed and lung injury was evaluated according to the lung wet/dry weight ratio and histological scoring system. Lung homogenates were harvested to detect the mRNA and protein expression of cytosolic phospholipase A2 (c‑PLA2) and CCSP. The content of arachidonic acid was measured using an ELISA. Following OLV treatment, c‑PLA2 expression was increased, CCSP expression was decreased and lung injury scores were significantly increased. Sevoflurane inhalation in the OLV‑treated group induced an upregulation of CCSP and a downregulation of c‑PLA2 expression. In the group NAO, in which the club cells were simultaneously exfoliated, OLV caused more severe lung damage and induced higher expression of c‑PLA2 compared with that in group O. However, sevoflurane inhalation reduced the extent of lung injury and the expression of c‑PLA2, even when the endogenous modulator of lung inflammation, CCSP, was exfoliated (group NAOF). These results indicated that OLV promoted lung inflammation through the CCSP and c‑PLA2 pathway. However, the results from the club cells exfoliated group indicated that the CCSP may not be involved in the protective effect exerted by sevoflurane inhalation.
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Copy and paste a formatted citation
Spandidos Publications style
Yang Y, Wang WF, Li YH, Li LS, Guo X and Liu R: Sevoflurane attenuates ventilator‑induced lung injury by regulating c‑PLA2 expression. Mol Med Rep 18: 2923-2928, 2018.
APA
Yang, Y., Wang, W., Li, Y., Li, L., Guo, X., & Liu, R. (2018). Sevoflurane attenuates ventilator‑induced lung injury by regulating c‑PLA2 expression. Molecular Medicine Reports, 18, 2923-2928. https://doi.org/10.3892/mmr.2018.9243
MLA
Yang, Y., Wang, W., Li, Y., Li, L., Guo, X., Liu, R."Sevoflurane attenuates ventilator‑induced lung injury by regulating c‑PLA2 expression". Molecular Medicine Reports 18.3 (2018): 2923-2928.
Chicago
Yang, Y., Wang, W., Li, Y., Li, L., Guo, X., Liu, R."Sevoflurane attenuates ventilator‑induced lung injury by regulating c‑PLA2 expression". Molecular Medicine Reports 18, no. 3 (2018): 2923-2928. https://doi.org/10.3892/mmr.2018.9243
Copy and paste a formatted citation
x
Spandidos Publications style
Yang Y, Wang WF, Li YH, Li LS, Guo X and Liu R: Sevoflurane attenuates ventilator‑induced lung injury by regulating c‑PLA2 expression. Mol Med Rep 18: 2923-2928, 2018.
APA
Yang, Y., Wang, W., Li, Y., Li, L., Guo, X., & Liu, R. (2018). Sevoflurane attenuates ventilator‑induced lung injury by regulating c‑PLA2 expression. Molecular Medicine Reports, 18, 2923-2928. https://doi.org/10.3892/mmr.2018.9243
MLA
Yang, Y., Wang, W., Li, Y., Li, L., Guo, X., Liu, R."Sevoflurane attenuates ventilator‑induced lung injury by regulating c‑PLA2 expression". Molecular Medicine Reports 18.3 (2018): 2923-2928.
Chicago
Yang, Y., Wang, W., Li, Y., Li, L., Guo, X., Liu, R."Sevoflurane attenuates ventilator‑induced lung injury by regulating c‑PLA2 expression". Molecular Medicine Reports 18, no. 3 (2018): 2923-2928. https://doi.org/10.3892/mmr.2018.9243
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